Institute of Technical Vocational Education Division of Applied Science TAN 213 FOOD MICROBIOLOGY Lecture 1 Tutorial Activity: Introduction, Concept Development, and Historical Development of Food Microbiology Lecture: Ms. PAIELA Student Name: Saki Wal Std ID#: 2019316024 1|Page TUTORIAL QUESTIONS/SUMMARY QUESTIONS 1. Write a brief summary of the introduction of Food Microbiology Microbiology is a specialized area of biology dealing with organisms too small to be seen without sufficient magnification. Microbiologists study bacteria, fungi, parasities, and virus, including their interaction with humans, animals, plants and the environment. While virus are not a living organisms like bacteria, fungi, and parasites, they are studied by microbiologist; therefore, we will use the term microbe (any microscopic entity that exibits the properties of life) to collectively refer to any of these biologically active and microscopic entities. In addition, there is an infectious agent responsible for numerous neurodegenerative diseases found in animals and humans known as ‘’prions’’. 2. List all the application of microbiology and briefly explain them. Food Microbiology Research studies focus on a variety microorganism that contaminate/damage food and those that can be used for food processing/modification among others. As such, microbiology gives special attention to such microorganisms as molds, yeast, bacteria among others that either benefit or have negative effects on the quality of food material with public health concern in mind. Medical Microbiology This the branch of microbiology that is concerned with the diagnosis, prevention of diseases caused by different types of organisms (infection agents). This sub-discipline is therefore related to a number of other fields including virology, bacteriology, and immunology. Industrial Microbiology This branch of microbiology is concerned with the use of given microorganisms for industrial production. Research studies directed towards the use of this organisms to increase and maximized yields in industries like fuel, pharmaceutical, and chemicals among others. The use of microorganisms makes it possible to make produce for a big market. Agricultural Microbiology 2|Page Agricultural microbiology is concerned with microbes associated with plants and animals diseases and production. As such, it is not only concerned with the medical significance of these organisms, but also their economic importance for farmers and industry as a whole. In the process, agricultural microbiology is aimed to solve issues identified in agricultural practices while helping yields for farmers. Soil Microbiology This is the branch of microbiology that deals with the study of soil microorganisms and how they impact soil properties. Pharmaceutical Microbiology Concerned with the use of microorganisms for inhibiting contamination as well as the development of pharmaceuticals Veterinary Microbiology Focus on microbes that causes disease in domestic animals Microbial Biotechnology Area of microbiology and biotechnology aimed at using microbes for beneficial purposes: It is aimed at enhancing microbial application in day to day life. A good example of this is the research study currently being conducted (at the time of writing) to use given bacteria to replace the traditional sewage systems. 3. Define the following concept of food microbiology I. Food – Any nutritious substance that people or animals eat or drink or those plants absorb in order to maintain life and growth. II. Microbiology – is the study of all living organisms that are too small to be visible with the naked eye but can only be seen with the help of a magnifying lens or a microscope. This includes bacteria, archaea, viruses, fungi, prions, protozoa and algae, collectively known as ‘’microbes’’ and their activities: Nutrition, Multiplication, Pathogenicity, control, etc. III. Food microbiology – is the study of the microorganisms that inhibit, create or contaminate food, including the study of microorganisms causing food spoilage, pathogens that may cause disease especially if food is improperly cooked or stored, those used to produce fermented foods such as cheese, yogurt, bread, beer, and wine and those with other useful roles such as producing probiotics. 3|Page Microorganisms – they are very minute organisms that are too small to be seen with IV. the naked eye. They are also refer to as microbes. They are observed with the help of a magnifying device called the microscope. 4. Discuss ‘’ Spontaneous Generation Theory’’ Controversy similar to the one concerning the origin of life was witness in the case of microorganisms. The theory or concept of ‘’spontaneous generation’’ was put forward and believed by many scientists (proposed by Aristotle) centuries. They believed that life arose spontaneously from pre-existing life and so the term ‘’abiogenesis’’. Meanwhile, there were opponents of this idea. The controversy was on for over 100 years. 5. Define ‘’Golden Age of Microbiology’’ The proof of microorganisms as the real etiological agents of infectious diseases marks the zenith of the period between 1850 and 1915 known as the Golden Age of Microbiology. 6. Summarise the historical development of general microbiology in the table in the order in which the ‘’ Spontaneous Genertation Theory’’ was disproved. Name of the Titles pioneer Year of Proposed Successful Discovery theories Experiments 1675 Described microbiologist Anthony Van Leeuwenhoek Dutch draper Microscopist Microbiologist the He was the first ‘’little animals’’ man to observe (animalcules) he and observed microorganisms with describe The Father of his crude home- using his single- Microbiology made lens which 4|Page microscope magnified while examining 300X rain water and magnification. 50- watery infusions. Between He wrote series 1674-1723 of papers describing his observations of bacteria, algae, protozoa, and fungi (Animalcules). Louis Jablot Professor of During Mathematics at period and lectured on the 1680-1716 perspective, Ecole Natioale des the Jablot explored optics, and Beaux-Arts geometry, leading to his personal pursuit From 1718 of microscopy. His landmark work presented his own developments and modifications of the microscope, observations of protozoa, and his opposition to 5|Page the theory of spontaneous generation. His new microscope permitted precise focusing by eliminating stray light and enable the mounting a diverse array of specimens. The microbes become clearer and more visible. He would refer to microbes he observed in terms such as ‘’fishes’’ or ‘’caterpillars’’, or even provide mirthful names such as, ‘’slipper’’, or ‘’bagpipes’’. In 1670 6|Page Jablot conducted an experiment in which he divided a hay infusion that had been boiled into two containers: heated a container that was closed to the air heated that and a container was freely open to the air. Only the vessel developed microorganisms. This further helps to disapprove abiogenesis Fancesco Redi Italian scientist 1868 After many smart experiments, disproved theory the of abiogenesis. Joseph Lister Professor of Applied Pasteur’s Surgery in observations Glasgow to the prevention of 7|Page Father of Aseptic Surgery wound sepsis. 1867 He discovered the use antiseptic technique to kill bacteria in wound and air with carbolic acid Robert Koch Father of Most of his Medical work was Microbiology between 1876 German and 1884 scientist 1876 He discovered the anthrax bacillus ahead of Pasteur and two of them had become rival. Louis Pasteur A French Chemist Between 1860 Performed series and 1890 of brilliant The Father of researches and Morden experiments for Medicine the wine industry 7. Briefly summaries the history of the Discover Food Microbiology The first person to really appreciate and understand the causal relationship between microorganisms in infusions and the chemical changes that took place in those was Louis Pasteur. Through his experiments, Pasteur convinced the scientific world world 8|Page that all fermentative processes were caused by microorganisms and that specific types of fermentations (e.g. alcohol, lactic or butyric) were the results of specific types of microorganisms. 9|Page