CHAPTER 2: CELLS CELL STRUCTURES All organisms are made of cells. Cells are the fundamental units of life. CELLS are the basic building blocks of all living things. The human body is composed of trillions of cells. They provide structure for the body, take in nutrients from food, convert those nutrients into energy, and carry out specialized functions. Cells contain the body’s hereditary material and can make copies of themselves. Cells provide six main functions. They provide structure and support, facilitate growth through mitosis, allow passive and active transport, produce energy, create metabolic reactions and aid in reproduction. i. Provide Structure and Support Like a classroom is made of bricks, every organism is made of cells. While some cells such as the collenchyma and sclerenchyma are specifically meant for structural support, all cells generally provide the structural basis of all organisms. For instance, skin is made up of a number of skin cells. Vascular plants have evolved a special tissue called xylem, which is made of cells that provide structural support. ii. Facilitate Growth Through Mitosis In complex organisms, tissues grow by simple multiplication of cells. This takes place through the process of mitosis in which the parent cell breaks down to form two daughter cells identical to it. Mitosis is also the process through which simpler organisms reproduce and give rise to new organisms. iii. Allow Passive and Active Transport Cells import nutrients to use in the various chemical processes that go on inside them. These processes produce waste which a cell needs to get rid of. Small molecules such as oxygen, carbon dioxide and ethanol get across the cell membrane through the process of simple diffusion. This is regulated with a concentration gradient across the cell membrane. This is known as passive transport. However, larger molecules, such as proteins and polysaccharides, go in and out of a cell through the process of active transport in which the cell uses vesicles to excrete or absorb larger molecules. Some of the smallest organisms, such as yeast and bacteria, are single-celled organisms (Prokarytic), but most plants and animals are multicellular (Eukaryotic). The two major types of cells are: Prokaryotic cells and Eukaryotic cells. The most important distinction between these 2 cells is considered to be the most important distinction among groups of organisms. o o Prokaryotes are single-celled (unicellular) organisms (e.g. Bacteria and Archaebacteria) that lack a membrane-bound nucleus, mitochondria, or any other membrane-bound organelle. Eukaryotes are organisms (Animals, Plants, Fungi and Protists) whose cells (multicellular) have a nucleus enclosed within membranes, unlike prokaryotes, which have no membrane-bound organelles. Prokaryotes were the only form of life on Earth for millions of years until more complicated eukaryotic cells came into being through the process of evolution. iv. Produce Energy An organism's survival depends upon the thousands of chemical reactions that cells carry out relentlessly. For these reactions, cells require energy. Most plants get this energy through the process of photosynthesis, whereas animals get their energy through a mechanism called respiration. Cells have many parts, each with a different function. Some of these parts, called organelles, are specialized structures that perform certain tasks within the cell. Human cells contain the following major parts: o NUCLEUS It is a circular structure that contains DNA (deoxyribonucleic acid) in the form of chromosomes, the cell’s hereditary (genetic information) material. The nucleus is found in the cytoplasm. o o This helps the cell make the right sorts of proteins. The information is kept on the chromosomes, which are inherited from the organisms’ parents. The chromosomes are made of DNA. o Mitochondria are the ‘POWERHOUSE OF THE CELL’, found in almost all cells, except those of Prokaryotes (Prokaryotes are organisms made up of cells that lack a cell nucleus or any membrane-encased organelles, e.g. Bacteria – Tuberculosis (TB) and Cholera). Chromosomes are very long, but extremely thin that they cannot be seen even by using an electron microscope. However, when the cell is dividing, they become short and thick, and can be seen with a good light microscope. o Inside them, oxygen is used to release energy from glucose, in the process called aerobic respiration. o Cells that use a lot of energy have a lot of mitochondria. Examples: Muscle cells are tightly packed with mitochondria. Sperm cells, which need energy to swim to the egg Nerve cells (Neurones), which need energy to transmit impulses Serves as the cell’s command/control center, which controls cell division, cell development and cell activities. CYTOPLASM The black spots in the electron microscope image (Figure 2.8) are granules of a carbohydrate called Glycogen. This is similar to starch, as starch is NEVER found in animal cells – they store Glycogen instead. Glycogen is a reserve of fuel. When required, it can be broken down to Glucose, to be used as a fuel by the mitochondria in the liver cell, or transported in the blood to other cells that need it. Within cells, the cytoplasm is made up of a jelly-like fluid (called the cytosol) which contains particles (especially proteins) and other structures (organelles) that surround the nucleus. Many different metabolic reactions (the chemical reactions of life) take place in the cytoplasm. Cytoplasm is nearly all water (70% is water in many cells) The cell membrane (cell surface membrane) is a thin semi-permeable membrane (outer lining of the cell – made of protein and fat) that surrounds the cytoplasm. Cytoplasm is contained by the cell membrane All cells have a cell membrane (also called cell surface membrane) around the outside. o Very difficult to see cell membrane in a plant cell because it is right against the cell wall. Its function is to separate the cell from its environment and allows materials to enter and leave the cell. o Cell membrane is important because it controls what enters and leaves the cell. o It protects the integrity of the interior of the cell by allowing certain substances into the cell while keeping other substances out. CELL MEMBRANE (CELL SURFACE MEMBRANE) MITOCHONDRIA Mitochondria (singular: Mitochondrion) are complex organelles that convert energy from food into a form that the cell can use. Mitochondria are tiny structures that are almost impossible to see with a light microscope, thus electronic micrographs are used. They have their own genetic material, separate from the DNA in the nucleus, and can make copies of themselves. The main difference between cell wall and cell membrane is, cell membrane is present in all cells but cell wall is only present in plants, bacteria, fungi and algae. It is not present in the Protozoa and animal cell.. Cell wall (fully permeable) covers the cell membrane of plants plants, which is a partially permeable (semi-permeable) membrane membrane. RIBOSOMES Ribosomes are a cell structure that makes protein (Proteinsythesis - Proteinss are made by joining small molecules called amino acids together. This process is called protein synthesis and happens in small structures in the cytoplasm of all cells called ribosomes. o Ribosomes are found in all types of cells – bacteria, protoctists, fungi, plants and animals all have ribosomes in their cells. o Protein is needed for many cell functions such as repairing damage or directing chemical processes processes. o Ribosomes can be found floating within the cytoplasm or attached to the endoplasmic reticulum reticulum. Ribosomes are organelles that process the cell’s genetic instructions to create proteins. o Ribosomes can float freely in the cytoplasm or be connected to the endoplasmic reticulum reticulum. Endoplasmic reticulum (ER) is a type of organelle found in eukaryotic cells (An organism whose cells contain a nucleus surrounded by a membrane and whose DNA is bound together by proteins (histones) into chromosomes) that forms an interconnected network of flattened, membrane-enclosed enclosed sacs or tube-like like structures known as cisternae cisternae. The membranes of the ER are continuous with the outer nuclear membrane, flattened disks organelles that help process molecules created by the cell. The endoplasmic reticulum also transports these molecules to their specific destinations either inside or outside the cell. Rough Endoplasmic Reticulum is the laye layer that surrounds the nucleus and has little black dots (Ribosomes) attached on to it. Smooth Endoplasmic Reticulum is the layer that is found away from the nucleus, closer to the cell membrane and does not have black dots (Ribosomes) attached to them. Ribosomes are even tinier structures/organelles than mitochondria and can only be seen with an electron microscope (Figure 2.10). o Ribosomes look like tiny dots attached to a network of membranes that run throughout the cytoplasm cytoplasm. This network is called the rough ugh endoplasmic reticulum. Plant cells contain all the Animal cells mentioned and defined above, but also have 3 additional parts: 1. Cell wall 2. Chloroplast, and 3. Vacuole CELL WALL A cell wall is a tough, fully permeable, permeable outermost layer of cells in plants, bacteria, fungi, and many algae that gives shape to the cell and protects it from infection and bursting when the cell takes in too much water. water Cell wall is made out of cellulose, determines tissue texture, and often is crucial to cell function. o Cellulose belongs to a group of substances called Polysaccharides. o Cellulose forms fibres which criss-cross criss over one another to form a very strong covering to the cell. The above is a picture of cellulose fibres of a plant cell wall CHLOROPLAST Chloroplasts are tiny plant powerhouses that capture light energy to produce the starches and sugars that fuel plant growth. They are found inside plant cells (within the cytoplasm) in plant leaves and in green and red algae as well as in cyanobacteria. o Chloroplasts are only found in the parts of the plant that are capable of Photosynthesis. o The majority of chloroplasts are found in the lea leaves of the plant because these structures have the greatest surface area for absorption.. The outer part of a plant stem may also contain chloroplasts. Chloroplasts allow plants to produce the complex chemicals necessary for life from simple, inorganic substances tances such as carbon dioxide, water and minerals. Chloroplasts contain the molecule chlorophyll chlorophyll, which are responsible for absorbing sunlight for photosynthesis photosynthesis. Chloroplasts often contain starch grains,, which have been made by photosynthesis. Animal cells never contain starch grains.. Some animal cells, however do have granules (tiny grains) of another substance similar to starch grains, called glycogen (found in the cytoplasm). Table shows some feature comparison of Plant Cells and Animal Cells: CELL TYPE A vacuole is membrane-bound bound organelle in the cytoplasm of most cells, especially plant cells, containing water and dissolved substances such as salts, sugars, enzymes, and amino acids. o Plant cells have very large vacuoles,, which contain a solution of sugars and other substances,, called CELL SAP. o Animal cells have much smaller maller membrane membrane-bound spaces, called VESICLES, which contain food or water water. A full vacuole presses outwards on the rest of the cell, and helps to keep the cell in shape. (have STARCH GRAINS) GRAINS (never have starch grain, but sometimes GLYCOGEN GRANULES) (have larger vacuoles containing CELL SAP) SAP (have smaler bound spaces containing food and water, called VESICLES) REGULAR SHAPE IRREGULAR SHAPE CYTOPLASM RIBOSIMES MITOCHONDRIA ROUGH ENDOPLASMIC RETICULUM (ER) SMOOTH ENDOPLASMIC RETICULUM (ER) CELL MEMBRANE CELLULOSE CELL WALL CHLOROPLAST (with CHLOROPHYLL) CELL SHAPE ANIMALS NUCLEUS VACUOLE VACUOLE PLANTS MICROSCOPES CELLS AND ORGANISMS One of the complexities of life as a multicellular organism is that the trillions of bits and pieces that make up your body must somehow all work together to accomplish the basic functions that keep you alive. i. Biologists refer to the relationship between cells, tissues and organs as the levels of organization of the human body. In the human body, cells are the basic units of life. Groups of cells working together for a specific function form tissues. Organs are two or more tissues operating together. Even separate organs work together, forming body systems. CELLS FORM TISSUES Groups of cells organized together for a specific function form tissues. There are four basic types of tissue in the human body: i. Epithelial - Epithelial tissue covers the exterior of the body as well as the linings of the organs and cavities of the body, ii. Muscle - Muscle tissue contains cells that are sometimes called “excitable” because they are able to contract and enable movement iii. Nerve - Nerve tissue conducts electrical impulses and send signals through the body iv. Connective - Connective tissue holds the body together and includes both bones and blood. EXAMPLE: Stomach Lining is a tissue, a layer of cells lining your stomach. These cells make enzymes to help digest your food. The stomach also has other tissues. For example, the layer of muscle in the stomach wall, made of cells which can move. o This muscle tissue makes the walls of the stomach move in and out, churning the food and mixing it up with the enzymes. The stomach also has other tissues. For example, the layer of muscle in the stomach wall, made of cells which can move. o This muscle tissue makes the walls of the stomach move in and out, churning the food and mixing it up with the enzymes. In plant anatomy, tissues are categorized broadly into three tissue systems: the epidermis, the ground tissue, and the vascular tissue. Epidermis tissue - Cells forming the outer surface of the leaves and of the young plant body. E.g. epidermis tissue from an onion bulb, Palisade tissue – a layer of cells in a leaf in which the cells are specialized to carry out photosynthesis Vascular tissue - a term referring to the parts of plants that convey water and nutrients from one part of the organism to another (Xylem and Phloem). Xylem tissue consists of a variety of specialized, waterconducting cells known as tracheary elements. TISSUES FORM ORGANS An organ is two or more tissues that come together to form a single unit with a unique structure and function. The heart, for example, is an organ that contains all four types of tissue (Epithelial, Muscle, Nerve and Connective tissues) to accomplish its very important task. There are 78 organs in the human body, including five organs considered vital for life. There are 5 vital organs considered vital for life: brain, heart, lungs, kidney, and liver. The largest human organ is the skin, which can weigh around 20 pounds. Of course, the levels of organization of the human body don’t stop with the organs. Individual organs work together in nine major organ systems. And, at the very top of the ladder, all those systems, organs, tissues and cells come together to form an organism: you! There are 4 organs in the Plant: the leaf, stem, root, and reproductive structures. ORGANS FORM ORGAN SYSTEMS An organ system is a group of organs that work together as a biological system to perform one or more functions. Each organ does a particular job in the body, and is made up of distinct tissues. There are 11 major organ systems in the human body. The most important systems are: o Nervous System is the main command system that directs function for all other systems. The nervous system sends signals throughout the body to control function and movement. It is composed of the brain, spinal cord and peripheral nervous system. It directs quick responses to stimuli, such as automatic reflexes. The nervous system works in conjunction with the endocrine system to control metabolism and other body functions. o Endocrine System uses chemical messengers. It secretes hormones into blood and other body fluids. Water balance, body growth and responses to stress are some of the activities regulated by the endocrine system. Glands that secrete hormones include the pituitary, thyroid, adrenal, pancreas and hypothalamus. o Cardiovascular system (Circulatory system) is occasionally referred to as the circulatory system. It contains the heart, blood vessels and blood. The blood transports nutrients, hormones, gases and waste products by using the blood vessels. The heart pumps the blood throughout the body and maintains blood pressure. Arteries pump blood away from the heart, and veins return blood toward the heart. o Respiratory system contains the nasal cavities, throat areas and lungs. The pharynx is shared with the digestive stive tract. Air moves from the pharynx to the larynx, which protects the opening to the trachea. The trachea is the main passageway to the lungs. It acts as an air filter. Inside the lungs, oxygen is extracted from the air, and carbon dioxide is exhaled as a waste product. o Digestive system food is absorbed and processed by the body. After being swallowed through the mouth, food moves through the esophagus and into the stomach.. The stomach breaks down the food mechanically and chemically so it can be digest digested by the small intestine and used for nutrition. Any undigested material is then moved through the large intestine and excreted through the anus. The liver is also considered part of the digestive system. It releases bile to aid in digestion. A plant has two organ systems: 1) the shoot system, and 2) the root system: o Shoot system is above ground and includes the organs such as leaves, buds, stems, flowers (if the plant has any), and fruits (if the plantt has any) any). o Root system includes those parts of the plant below ground, such as the roots, tubers, and rhizomes. ORGANS SYSTEMS FORM ORGANISMS The way in which organisms are built up can be summarized in the diagram below: Figure above: From an atom to the entire Earth, biology examines all aspects of life. RELATING SPECIALISED CELL STRUCTURE TO THEIR FUNCTIONS (ADAPTATION) ROOT HAIR CELLS Plants absorb water from the soil by osmosis. They absorb mineral ions by active transport, against the concentration gradient. Root hair cells are adapted for taking up water and mineral ions by having a large surface area to increase/maximise /maximise the rate of absorption. Root hair cells are found on the root of a plant are a on the edge of the root and a little way up from the root tip. CILIATED CELLS Ciliated epithelial cells are covered with cilia, which are produced by the cytoplasm, and are tiny hairs that push dust, mucus, bacteria, or hair up the trachea to the back of the throat where they can be swallowed swallowed. o o They are long epidermal cells o They don’t live for very long and are replaced by new ones as the root grows. Water moves into the root hair via osmosis. The cytoplasm and cell sap inside the root hair is quite concentrated with mineral ions which enters the root hair by active transport. o The water is absorbed into the root by the root hairs and taken to the Xylem vessels to be transported to other parts f the plant. Ciliated cells are designed to stop damage to the lungs and other organs. Ciliated cells are found in epithelial surfaces (Epithelial tissues line the outer surfaces of organs and blood vessels throughout hout the body, as well as the inner surfaces of cavities in many internal organs) of the Respiratory system in such structures as the trachea, bronchial tubes, and nasal cavities. o It often occurs in the vicinity of mucus-secreting secreting goblet cells – mucus trapss the dust and bacteria and moved away by the mucus. Goblet cell is a column-shaped shaped cell found in the respiratory and intestinal tracts, which secretes the main component of mucus. Cilia move rhythmically; they beat constantly, moving waste materials such as dust, mucus, and bacteria upward through the airways, away from the lungs and toward the mouth. XYLEM VESSELS The xylem transports water and minerals from the roots up the plant stem and into the leaves. leaves In a mature flowering plant or tree, most of the cells that make up the xylem are specialised cells called vessels. Xylem vessel have no cytoplasm or nuclei. Their walls are made of cellulose and lignin. o Lignin is very strong substance, so the xylem vessels help keep plants upright. Wood is made almost entirely of lignified xylem vessels. PALISADE MESOPHYLL CELLS The palisade mesophyll cells are plant cells located on the leaves, the upper part of the leaf, right below the epidermis and cuticle (in the palisade mesophyll layer) and is where most of the photosynthesis takes place. Palisade cells contain a lot of chloroplasts, which contains chlorophyll, to help them perform photosynthesis. o The many chloroplasts circulate round the palisade cell, which maximizes the absorption of light. o Moving chloroplasts helps to avoid shadowing effect. Palisade cells are closely packed together to maximize light absorption. o Palisade cells have large vacuole which pushes the chloroplasts and cytoplasm to the edge of the cell. o Xylem is located in roots, stems and leaves of the plant and it transports water and minerals from plant roots all the way to the leaves. o With phloem it forms vascular bundles. Dead cells in Xylem contribute to wooden parts of the plant. Xylem vessel is like a long drain pine (see above Figure 8.3) made up of many hollow, dead cells joined end to end (see Figure 8.2 below). They don’t live for very long and disappear over time (as the plant grows and replaces new ones) to become a hollow structure/vessels. o As the content of the cells die, it creates the hollow vessel that allows transport of water and nutrients to the rest of the plant all the way to the leaves. Palisade cells are long and thin, arranged side by side near the top surface of the leaf This exposes the chloroplasts more to enable them to absorb more light energy NERVE CELLS (NEURONE) RED BLOOD CELLS The human Nervous system is made of special cells called neurones or nerve cells (Figure 13.2 below is a particular of neurone called a motor neurone). Blood is a complex liquid tissue. It transports oxygen, dissolved substances and heat around the body. It is also involved in the body’s immune response. Nerve cells (Neurones) carry (conduct) impulses (messages) from one part of the body to another. Neurones need to be able to rapidly transmit electrical impulses along their length. They also need to exchange signals with neighbouring neurones across the gaps, known as synapses, that separate them. Red blood cells transport oxygen for aerobic respiration (respiration that requires oxygen). They must be able to absorb oxygen in the lungs, pass through narrow blood vessels, and release oxygen to respiring cells. Nerve cells/Neurones contain the same basic parts as any animal cell – Nucleus, Cytoplasm, and a Cell Membrane. However, their structure is specifically adapted to be able to carry messages very quickly. To achieve these functions, nerve cells have several adaptations. o The axon, the long, thin part of the neuron along which the nerve impulse passes, is covered in a fatty myelin sheath, which acts as an electrical insulator. This increases the speed of transmission by forcing the impulse to jump between gaps in the sheath, known as Nodes of Ranvier, rather than passing along the full length of the axon. o Nerve cells also have a lot of mitochondria, which provide the energy to synthesise neurotransmitters such as acetylcholine that pass messages across synpases. o In addition, numerous fine/shorter fibre extensions to the cell body at one end of the cell, known as dendrites or dendrons, provide a high surface area allowing the nerve cell to form synapses with many others. Similar branching occurs at the other end of the cell, and synaptic knobs at the end of branches store neurotransmitter, ready to diffuse across the synapse as soon as an impluse arrives. Red blood cells have adaptations that make them suitable for this: Adaptations for efficient diffusion of oxygen Red blood cells have very thin cell membranes – this lets oxygen diffuse through quickly. o The cells themselves are thin, so there is only a short distance for the oxygen to diffuse to reach the centre of the cell. The biconcave shape provides a large surface area compared to the volume of the red blood cell, allowing diffusion to happen efficiently. Adaptations for efficient carriage of oxygen Red blood cells do not contain a nucleus so they can contain more haemoglobin. o Oxygen combines with haemoglobin to form oxyhaemoglobin: o Oxygen binds reversibly to oxygen. SPERM CELLS Humans typically reproduce through sexual reproduction. Sexual reproduction produces offspring that are unique individuals. Half of their genes come from each parent. In sexual reproduction, a male gamete and a female gamete join together. This is fertilisation. A gamete is a mature haploid male or female germ cell which is able to unite with another of the opposite sex in sexual reproduction to form a zygote. o o Gametes are the sex cells. Gametes have adaptations to increase the chances of fertilisation and successful development of an embryo. The male gametes, called spermatozoa or sperm, are made in two testes (singular testis). These are outside the body, in two sacs of skin called the scrotum. Sperm cells have these adaptations: o sperm cells are produced in large numbers to increase the chance of fertilisation. o a tail to move them towards an egg cell o many mitochondria to provide energy o an acrosome (part of the tip of the head) that releases enzymes to digest the egg membrane. o Sperm cell also has a nucleus, which contains the genetic material (23 chromosomes) for fertilization. EGG CELLS The ovary produces egg cells which are the female gametes. The main function of egg cells is to join with male cells (sperm) for the purpose of reproduction. Adapted to have: o The egg cell is large in size because it stores nutrients. o has cytoplasm which has york to stores energy (Figure 17.6) The egg cell nucleus consists of half the number of chromosomes (23) that are found in female body cells. o Walls are thin to allow the sperm to penetrate. VIDEOS & SUPPLEMENT MATERIAL CELLS STRUCTURE FOR DUMMIES https://youtu.be/URUJD5NEXC8 DIFFERENCES BETWEEN EUKARYOTIC AND PROKARYOTIC CELLS https://youtu.be/4ASXK6RG_f0 CELL STRUCTURE AND ORGANIZATION Https://youtu.be/dkWhwnLpquY THE STRUCTURE AND FUNCTION OF A CELL https://sciencing.com/structure-function-cell5105947.html ORGANELLES OF CELLS https://youtu.be/RKmaq7jPnYM CELL STRUCTURE OF ANIMALS https://sciencing.com/cell-structure-definitions5043056.html LEVELS OF ORGANIZATION OF SPECIALIZED CELLS https://www.youtube.com/watch?v=HBlk_f0v4Q&pbjreload=10 RELATIONSHIP BETWEEN CELL STRUCTURE AND FUNCTION https://sciencing.com/relationship-between-cellstructure-function-5154975.html IGCSE SPECIALIZED CELLS https://www.youtube.com/watch?v=tLPOHbRreQU DIFFUSION: WHAT IS IT AND HOW DOES IT HAPPEN? https://sciencing.com/diffusion-what-is-it-how-does-ithappen-13718021.html ACTIVE TRANSPORT: AN OVERVIEW https://sciencing.com/active-transport-an-overview-ofprimary-secondary-13718016.html METABOLIC REACTIONS: ANABOLIC AND CATABOLIC CELL METABOLISM https://sciencing.com/anabolic-vs-catabolic-cellmetabolism-definition-examples13717911.html?q2201904 5 STAGES OF MITOSIS https://sciencing.com/5-stages-mitosis-13121.html WHAT IS MEIOSIS https://sciencing.com/what-is-meiosis-13714447.html THE NEURONE http://youtu.be/1bC0FGa0W0