LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2007
BT 2814/BT 2807 - BIOPROCESS AND ENZYME TECHNOLOGY
Date & Time: 19/04/2007 / 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART A
Answer all the Questions
I. Choose the correct answer:
(20 marks)
(5x1=5)
1.The enzyme β- galactosidase is produced by
(a) Bacillus cereus (b) E.coli (c) Yeast (d) Salmonella sp.
2. Which one of the following is a secondary metabolite?
(a) Carbohydrate (b) protein (c) nucleotide (d) antibiotic.
3. The Roll-bottle technique is used to produce sporulation on
(a) Solidified media
(b) semi- solid media
(c) Submerged media
(d) liquid media.
4.Lip-seal method of sealing is made for----------- joints.
(a)glass-glass (b) glass-metal (c) metal-metal (d) metal-fibre
5.The aerobic process of biological treatment used in the effluent treatment is
(a) Sludge blankets (b) Imhoff –tanks (c) trickling filters (d) sludge digestors.
II. State True or False; if false state the reason.
(5x1=5)
6.The solubility of an enzyme can also be increased by the addition of its substrate.
7. Mass transfer occurs in mixturers containing local concentration variations.
8. Osmotic shock is a mechanical method of cell disruption.
9. Diethyl barbiturate acts as an inhibitor for rifampicin.
10.The ‘Prochem maxflo T’ is a conventional agitator.
III. Complete the following:
(5x1=5)
11. The production of healthy state inoculum is called-----------------------------12. The genetic modification for the strain improvement can be achieved by --------------and --------------------------.
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IB 14
13. The ------------------------- type of sparger has perforation pipes which may be cross or
ring type.
14. The method of separating the amphoteric substances based on their isoelectric point
is called ---------------------------------.
15. --------------------------- is used to measure the airflow rate in bioprocess control.
IV. Aswer the following questions each within 50 words. (5x1=5)
16.Write notes on amylase.
17. Define del or nabla factor.
18.What are antifoams?
19. Give short notes on sparger.
20.What are off- line sensors?
PART B
V. Answer any five of the following, each within 350 words.
(5x8=40)
21. Give an account on the industrial applications of enzymes.
22. Bring out the significance the computer aided control in fermentation technology
23. Explain the various methods of immobilizing the enzymes.
24.Write a brief note on fermentation economics.
25. Describe the working mechanism and applications of airlift bioreactor.
26. Describe the methods of cell disruption techniques.
27. Brief out the process of strain improvement using mutation.
28. Give an account on the secondary treatment process for the effluent.
PART C
VI. Answer the following each in about 1500 words.
(2x20=40)
29. (a) Describe the enzyme production process using submerged and solid state
fermentation.
(or)
(b) Give an account the media formulation and optimization.
30. (a) Describe in detail the construction of a bioreactor and list out the various types.
(or)
(b) Explain in detail about the down stream processing in fermentation technology.
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