LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH.
SIXTH SEMESTER – APRIL 2008
PB 6606 - FERMENTATION TECHNOLOGY
Date : 23/04/2008
Time : 9:00 - 12:00
Dept. No.
KL 25
Max. : 100 Marks
PART – A
Answer all questions
(20 marks)
I. Choose the best answer
(5x1=5)
01. For fermentation using fungus the fermentor best suitable is
a) Cyclone column b) Packed tower
c) Airlift
d) Memberane
02. In small labs the culure of microbes are preserved by
a) Lyophilisation
b) Subculture every 3 months
c) Liquid nitrogen
d) At room temperature
03. Fermentors are usually made of
a) Copper
b) Brass
c) Gun metal
d) Stainless steel
04. The antifoam agent added to control foaming in fermentation
a) Soap water b) Lard oil
c) Glucose syrup
d) Enzymes
05. HPLC stands for
a) High Performance Liquid Chromatography
b) High Premium Liquid Chromatography
c) High Performance Liquid Carrier
d) High Pressure Liquid Carrier.
II. State whether the following are true or false
(5x1=5)
06. Biocon in India uses fermentation for production of its products.
07. Parasexual cycle of yeast is used for strain improvement.
08. Aerators and agitators have the same function in a fermentor.
09. Strong acid and alkali should not be used in adjusting pH during fermentation.
10. Glass beads are used for the disruption of cells during down stream processing.
III. Complete the following
(5x1=5)
11. Chemical sterilants like _________________ are used to clean the fermentor.
12. Oxygen pressure is measured by __________________.
13. Spargers are used in fermentation when _______________ cause damage to cells.
14. Virus required for vaccination is preserved in ________________.
15. ____________ is the best method of purification of enzyme.
IV. Answer the following in on or two sentences
16. What is a biosensor?
17. Differentiate between valves and steam traps.
18. Which are the salts used for enzyme precipitation?
19. What is transformation process?
20. Define growth kinetics.
(5x1=5)
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PART – B
(5x8=40)
Answer any five of the following in 350 words, draw diagram wherever necessary)
21. Give an account of the range of fermentation.
22. Write about the chronological development in fermentation industry.
23. Explain 1) Tower fermentor
2) Air lift fermentor
24. Comment on 1) Aerators and agitators
2) Valves and steam tap
25. Explain the growth kinetics in batch and continuous cultures.
26. Explain media composition in industrial fermentation and media sterilisation.
27. Describe the types of biosensors and its working.
28. Describe the chromatographic technique used in down stream processing.
PART –C
(2x20=40)
Each not exceeding 1500 words, draw a diagram wherever necessary
29. a) Describe
i) Biomass in fermentation ii) Recombinant products
OR
b) Explain the different parts of the fermentor with its functions.
30. a) Describe the isolation, improvement and preservation of industrially important microbes.
OR
b) Elaborate on the measurement of variables like temperature, pressure and flow
rate control.
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