LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2006
BT 2814 - BIOPROCESS AND ENZYME TECHNOLOGY
IB 33
(Also equivalent to BT 2807)
Date & Time : 21-04-2006/FORENOON
Dept. No.
Max. : 100 Marks
PART A
Answer all the questions
I. Choose the correct answer.
(20marks)
(5x1=5)
1.Saccharomyces cerevesiae produces the enzyme
(a) dextranase (b) invertase (c) uric oxidase (d) glucose isomerase.
2. The enzyme α- amylase is produced by the microorganism
(a) Aspergillus niger
(b) Xanthomonas campestris
(c) Bacillus amyloliquefaciens.
(d) Erymothecium ashbyii.
3. Which one of the following is an anionic exchanger?
(a) sulphonic acid
(b) carboxy-methyl cellulose
(c) DEAE
(d) sepharose
4.Sulphite waste liquor is produced from
(a) Paper pulping industry
(b) steeping of corn starch
(c) Molasses
(d) cane sugar.
5.The two-phase aqueous system is used for the extraction of
(a) lipase (b) uric oxidase (c) isomerase (d) penicillin acyclase.
II. State True or False; if false state the reason:
(5x1=5)
6. The enzyme papain is used as a meat tenderizer.
7. Phenoxyacetic acid is used as the precursor for Penicillin G.
8. Air-lift reactors are used extensively as immobilized enzyme reactors.
9. Polyelectrolytes are commonly used as flocculants in the chemical treatment
process.
10. Triton x-100 is a detergent.
III. Complete the following:
(5x1=5)
11. The secondary metabolites are produced during ---------------- phase of the growth
cycle.
12. The ----------------- detector contains a transmitter and receiver in foam control.
13. ------------------------ is used for the agitation process control.
14. An example of the mutagenic agent used in strain improvement is -----------------.
15. The recovery and purification of the biotechnological products for its intented use
is called ---------------------
IV. Answer the following questions each with in 50 words:
(5x1=5)
16. Write a note on Cellulase.
17. What is On-line sensor?
18. Explain Fick’s law?
I9. What is Tropophase?
20. What is Super-critical fluid extraction?
PART B
V. Answer any five of the following, each within 350 words:
(5x8=40)
21. Brief out the steps involved in the recovery of enzymes.
22. Explain the various ranges of fermentation process
23. Give an account on foaming and its control.
24. Illustrate the various methods of preserving the pure cultures.
25. Describe the applications of enzymes in immunology and biotransformation.
26. Explain the different types of chromatographic techniques.
27. What are biosensors? Give their applications.
28. Explain the mass, energy and momentum transfer rate
PART C
VI. Answer the following, each within 1500 words.
(2x20=40)
29. (a) Describe the various types and methods of enzyme immobilization and add a
note on the applications of immobilized enzymes.
(or)
(b) Enumerate the computer applications in fermentation technology.
30. (a) Give an account on the inoculum development for bacterial and mycelial
processes.
(or)
(b) Explain the biological effluent treatment and its disposal in sewered areas.
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