LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2008
BT 2814 - BIOPROCESS AND ENZYME TECHNOLOGY
Date : 22/04/2008
Time : 1:00 - 4:00
Dept. No.
SECTION – A
Answer all the questions
KL 31
Max. : 100 Marks
(20 marks)
I. Choose the best answer.
(5 X 1 = 5)
1. The enzyme α-amylase is produced by
(a) Aspergillus niger
(b) Erymothecium ashbyii
(c) Xanthomonas campestris
(d) Bacillus amyloliquefaciens.
2. Which one of the following is used to check the vortex and improve aeration in
fermenter?
(a) agitator (b) baffles
(c) sparger
(d) stirrer glands.
3. During the cryopreservation of the cells the survival rate of the cells can be increased
by addition of
(a) glycerol
(b) L-glutamate
(c) glycogen (d) sodium carbonate.
4. The freshness of the food can be analysed using the ____________ biosensor
(a) potentiometric (b) conductimetric
(c) thermometric
(d) amperometric.
5. The evaporator used for concentrating highly viscous product is
(a) falling-film evaporators
(b) plate evaporators
(c) forced-film evaporators
(d) centrifugal forced –film evaporators.
II. Say True or False; if false, state reasons.
(5 X 1 = 5)
6. The enzyme trypsin is used as a meat tenderizer.
7. The solid state fermentation is also called tray cultivation.
8. In gel permeation chromatography, the smaller molecules are entrapped in the voids of
the gel.
9. DEAE is a cationic exchanger.
10. Foaming reduces mass and heat transfer rates.
III. Complete the following.
(5 X 1 = 5)
11. The measure of electron activity in a fermentation process is called _____________.
12. _________________ chromatography allows the separation of proteins according to
their hydrophobicity.
13. The technique of pressure extrusion in solid shear is done using ___________ at lab
scale.
14. The primary metabolites are produced during the ____________ phase of growth
cycle.
15. Air-lift reactor works on the principle of ____________________ tube principle.
IV. Answer the following in one or two sentences, each in about 50 words. (5 X 1 =5)
16. Write short notes on cellulase.
17. Define precipitation.
18. What are antifoams? Give examples.
19. What is liquid-liquid extraction?
20. What are off-line sensors?
SECTION – B
V. Answer any five questions, each in about 350 words only.
(5 X 8 = 40)
21. Brief out the various methods of immobilizing the enzymes.
22. Give a brief note on media formulation in bioreactions.
23. Brief out the process of strain improvement using mutation.
24. Explain the chromatographic techniques used for metabolite purification.
25. Give an account on the physical and chemical environment in control and monitoring
instruments.
26. Write a brief note on fermentation economics.
27. Describe the applications of enzymes in immunological diagnostic assays.
28. Explain the working principle of biosensors and list any four types with its
applications.
SECTION – C
VI. Answer the following, each in about 1500 words.
(2 x 20 = 40)
29. a) Describe the enzyme production using submerged and solid-state fermentation.
(or)
b) Explain the construction of bioreactors in detail and list the types of configuration.
30. a) Explain the mass transfer effect of oxygen and heat. Add a note on its effect on
fermentation process.
(or)
b) “The solution for pollution is not a dilution”- Justify the statement in regard with
the industrial effluent treatment and its disposal in sewered areas.
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