LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
FIRST SEMESTER – NOV 2006
AE 14
BT 1807 - MICROBIOLOGY
Date & Time : 26-10-2006/1.00-4.00
Dept. No.
PART A
Answer all the questions
Max. : 100 Marks
(20 Marks)
I. Choose the correct answer:
(5x1=5)
1. The cell wall of fungi is made up of
(a) Chitin
(b) pectin
(c) polysaccharides (d) proteins
2. Diptheria toxin is an example of
(a) cytotoxin (b) endotoxin (c) enterotoxin
(d) neurotoxin.
3. The key – precursor used in the biosynthesis of glutamic acid is
(a) isocitrate (b) succinate (c) cis- aconitate
(d) α- ketoglutarate.
4. Which one of the following is a semi-synthetic penicillin?
(a) erythromycin
(b) ampicillin (c) chloramphenicol (d) spiromycin.
5. The ds DNA virus is
(a) Picorna
(b) Pox
(c) Corona
(d) parvo.
II. State True or False; If false state the reason.
(5x1=5)
6. Rhizosphere effect is an example of parasitic interaction.
7. The strain commercially used for the production of vitamin B12 is
Propionibacterium sp.
8. The greatest abundance of phosphorus in cells is with DNA.
9. Streptococcus salivarius possess both capsule and slime layer.
10. Methanobacterium contain psudomurein, made of N- acetylmuramicacid with
β- 1- 4 glycosidic bonds.
III. Complete the following:
(5x1=5)
11. Wool sorter’s disease is caused by ---------------------------------12. -------------- & ----------------------- are the toxins produced by E.coli.
13. The vacuoles of the plant cells are bounded by a single, semi permeable
membrane Known as ------------------------14. The outer membrane of the Gram-negative cell is anchored to the underlying
peptidoglycan by means of -----------------------15. The temperature for liquid nitrogen storage is ------------------IV. Answer the following questions each in about 50 words:
16. Give a short note on food intoxication.
17. What are thermophiles? Give example.
18. What is Tyndallization.?
19. Write short notes on steroids.
20. What is synergism?
(5x1=5)
PART B
V. Answer any five of the following questions each in about 350 words. (5x8=40)
21. Give a brief account on microbial toxins.
22. What are pure cultures? Describe the methods used to isolate pure culture.
23. Explain the commercial importance of Bacillus spp.
24. Give a brief account on Vaccine production.
25. Illustrate the biogeochemical cycling of sulphur.
26. Give an account on the Bacterial Single cell protein production.
27. Brief out the various methods of sterilization.
28. Describe the classification of bacteria based on environmental conditions.
PART C
VI. Answer the following questions each in about 1500 words:
(2x20=40)
29. (a) Describe the prokaryotic cell structure and function.
(or)
(b) Give a general account on virus structure, classification, and replication.
30. (a) Illustrate the penicillin biosynthesis in detail.
(or)
(b) Describe the biogeochemical cycling of Nitrogen.
***********