NAME_______________________________
MCB 2004
MICROBIOLOGY
TEST #2, MARCH 04, 2009
R. H. REEVES
IN THIS PART THERE ARE FORTY QUESTIONS WORTH TWO POINTS EACH.
THERE IS
ONLY ONE CORRECT ANSWER FOR EACH QUESTION.
PLEASE CIRCLE THE CORRECT
LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF THE QUESTION NUMBER.
THEN TRANSFER ALL YOUR ANSWERS TO THE ANSWER SHEET.
1.
THE THREE "KINGDOMS" OF ORGANISMS DEFINED BY 16S rRNA GENE
SEQUENCING ARE
A.
EUKARYA, PROTISTA AND ARCHAEA
B.
BACTERIA, PROTISTA AND ARCHAEA
C.
EUKARYA, MONERA AND BACTERIA
D.
EUKARYA, MONERA AND ARCHAEA
E.* BACTERIA, EUKARYA AND ARCHAEA
KEY FOR QUESTIONS 2-8. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH ANSWER
MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
CHLAMYDIA
BACILLUS
RICKETTSIA
PSEUDOMONAS
CORYNEBACTERIUM
B_____2.
AN ENDOSPORE FORMING BACTERIUM
B_____3.
MOST CLOSELY RELATED TO THE CLOSTRIDIA AND MYCOPLASMA
D_____4.
THIS BACTERIUM BELONGS TO THE BEST STUDIED OF ALL GROUPS
OF BACTERIA, THE -PROTEOBACTERIA
E_____5.
MOST CLOSELY RELATED TO THE BACTERIA THAT CAUSE
TUBERCULOSIS
C_____6.
AN OBLIGATE INTRACELLULAR PARASITE IN THE PROTEOBACTERIA
E_____7.
THIS BACTERIUM PRODUCES A POWERFUL TOXIN THAT INHIBITS
PROTEIN SYNTHESIS IN TARGET CELLS
A_____8.
NOT PHYLOGENETICALLY WITHIN THE "GRAM NEGATIVE" NOR GRAM
POSITIVE GROUPS OF BACTERIA
9.
TOXIN IS FOUND IN BOTH E. COLI AND B. PERTUSSIS
DIPHTHERIA TOXIN
CHOLERA TOXIN
TETANUS TOXIN
ENDOTOXIN
BOTULINUM TOXIN
THIS
A.
B.
C.
D.*
E.
MCB 2004
TEST #2
PAGE 2
10.
11.
ALL VIRUSES
A.
CONTAIN
B.
CONTAIN
C.* CONTAIN
D.
CONTAIN
E.
CONTAIN
THIS
A.
B.*
C
D.
E.
BOTH RNA AND DNA
AN ENVELOPE
A CAPSID
VIRAL-CODED SPIKES OR PEPLOMERS (GLYCOPROTEINS)
HOST PROTEINS
VIRUS HAS A PROTEIN BOUND TO THE 5'-ENDS OF ITS DNA
PICORNAVIRUS
ADENOVIRUS
TOGAVIRUS
POLYOMAVIRUS
ORTHOMYXOVIRUS
KEY FOR QUESTIONS 12-16. THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY
WORD MAY BE USED MORE THAN ONCE.
A.
B.
C.
D.
E.
HERPES SIMPLEX TYPE 2
RHINOVIRUS (COMMON COLD VIRUS)
SMALLPOX VIRUS
HUMAN PAPILLOMA VIRUS
INFLUENZA VIRUS
A_____12.
THE VIRION OF THIS DNA VIRUS HAS A POLYHEDRAL (ICOSAHEDRAL)
CORE (OR NUCLEOCAPSID) AND A MEMBRANE ENVELOPE
C_____13.
THIS VIRUS, DUE TO EFFORTS OF THE WORLD HEALTH
ORGANIZATION, NO LONGER INFECTS MAN
E_____14.
AN ENVELOPED RNA VIRUS
B_____15.
GENOME IS AN mRNA, WHICH CODES FOR A SINGLE POLYPROTEIN
A_____16.
CAUSE OF A LATENT AND RECURRENT SEXUALLY TRANSMITTED
DISEASE
17.
A MAJOR DIFFERENCE BETWEEN THE GROWTH OF VIRUSES AND CELLS IS
THAT VIRUSES
A.
REPLICATE BY BINARY FISSION
B.* GO THROUGH AN ECLIPSE PHASE
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
18.
A LYTIC RNA BACTERIOPHAGE
A.
TMV
B.
T4
C.
(lambda)
D.* MS2
E.
RSV
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TEST #2
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19.
VIROIDS
A.* INFECT ONLY PLANTS
B.
ARE COMPOSED OF DOUBLE-STRANDED DNA
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
20.
THIS VIRUS HAS A SEGMENTED RNA GENOME THAT CODES FOR 10
PROTEINS
A.
MUMPS VIRUS
B.
EASTERN EQUINE ENCEPHALITIS VIRUS
C.* INFLUENZA VIRUS
D.
RUBELLA VIRUS
E.
POLIOVIRUS
21.
IF SYMPTOMS DEVELOP THIS RNA VIRUS CAUSES A FATAL DISEASE WITH A
MORTALITY RATE OF 100%
A.
POLIOVIRUS
B.
RUBELLA VIRUS
C.
MUMPS VIRUS
D.* RABIES VIRUS
E.
INFLUENZA VIRUS
22.
IN A
A.*
B.
C.
D.
23.
BACTERIA THAT MAKE UP THE NORMAL FLORA ARE
A.* USUALLY COMMENSALS
B.
USUALLY FOUND IN THE BLOOD
C.
VIRULENT PATHOGENS
D.
ALL PARASITES
E.
NEVER OPPORTUNISTS
24.
BACTERIA THAT ARE NORMAL FLORA OF THE SKIN ARE ABLE TO TOLERATE
A.
HIGH pH (GREATER THAN pH 8.0)
B.* THE PRESENCE OF FATTY ACIDS
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
25.
NON-SPECIFIC DEFENSES AGAINST INFECTION INCLUDE
A.
THE pH OF THE STOMACH
B.
LYSOZYME IN TEARS
C.
FATTY ACIDS ON THE SKIN
D.
LAVAGING BY TEARS, URINE AND MUCOUS
E.*
ALL OF THE ABOVE
COMMENSALISTIC RELATIONSHIP BETWEEN A MICROBE AND A HOST
THE MICROBE BENEFITS
THE HOST BENEFITS
BOTH OF THE ABOVE
NEITHER OF THE ABOVE
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TEST #2
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26.
THE TWO MAJOR TYPES OF PHAGOCYTES AGAINST BACTERIA ARE
A.* MACROPHAGE AND NEUTROPHILS
B.
LYMPHOCYTES AND MACROPHAGE
C.
THROMBOCYTES AND MONOCYTES
D.
ERYTHROCYTES AND MONOCYTES
E.
LYMPHOCYTES AND THROMBOCYTES
27.
PHAGOCYTIC CELLS DESTROY BACTERIA AND VIRUSES
A.* WITH DEGRADATIVE ENZYMES
B.
WITH THE PRODUCTION OF HYDROCHLORIC ACID
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
28.
INTERFERON IS A PROTEIN THAT
A.
IS MADE BY CELLS INFECTED BY BACTERIA
B.
OPSONIZES BACTERIAL CELLS
C.* PROTECTS CELLS AGAINST VIRAL MULTIPLICATION
D.
CAUSES INFLAMMATION
E.
INTERACTS WITH ANTIGEN
29.
AN EXAMPLE OF ARTIFICIALLY ACQUIRED ACTIVE IMMUNITY IS
A.* DEVELOPING ANTIBODIES TO AN INJECTED TOXOID
B.
RECEIVING HORSE ANTISERUM (ANTITOXIN) TO A TOXIN
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
30.
THE CELL TYPE ASSOCIATED WITH THE CELL-MEDIATED IMMUNE RESPONSE
A.
PLASMA CELLS
B.* T-CELLS
C.
B-CELLS
D.
MACROPHAGE
E.
B-MEMORY CELLS
KEY FOR QUESTIONS 31-35. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH ANSWER
MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
ENDOTOXIN
B.
EXOTOXIN
C.
TOXOID
D.
ANTITOXIN
E.
HEMOLYSIN
C_____31.
USED AS A VACCINE
A_____32.
A TOXIN DERIVED FROM THE OUTER MEMBRANE OF BACTERIA
E_____33.
A PROTEIN THAT LYSIS RED BLOOD CELLS
D_____34.
OFTEN DERIVED FROM HORSE SERUM AND USED TO TREAT TOXINOSIS
B_____35.
A BIPARTITE PROTEIN THAT CAUSES SPECIFIC TOXINOSIS
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TEST #2
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36.
A MAJOR EFFECT OF ACTIVATED COMPLEMENT IS
A.
HEMOLYSIS
B.* OPSONIZATION
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
37.
HUMAN PRION DISEASES INCLUDE
A.
KURU
B.
CREUTZFELDT-JAKOB DISEASE
C.* BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
38.
THIS
A.*
B.
C.
D.
E.
39.
THE IgG ANTIBODY MOLECULE IS COMPOSED OF
A.
TWO POLYPEPTIDE CHAINS, BOTH LARGE
B.
TWO POLYPEPTIDE CHAINS, ONE LARGE AND ONE SMALL
C.
THREE LARGE POLYPEPTIDE CHAINS
D.
FOUR LARGE POLYPEPTIDE CHAINS
E.* FOUR POLYPEPTIDE CHAINS, TWO LARGE AND TWO SMALL
40.
THIS
A.
B.
C.
D.*
FULLY MATURED B-CELL ACTIVELY MAKES ANTIBODY
PLASMA CELLS
T-CELLS
B-CELLS
MACROPHAGE
B-MEMORY CELLS
STATEMENT ABOUT THE ANAMNESTIC RESPONSE IS TRUE
IgG INCREASES SLOWLY COMPARED TO THE INITIAL RESPONSE
IgM DOES NOT INCREASE AT ALL
BOTH OF THE ABOVE
NEITHER OF THE ABOVE
SHORT ANSWER QESTIONS ON NEXT PAGE – ANSWER TWO OF THREE
MCB 2004
TEST #2
PAGE 6
SHORT ANSWER QUESTIONS:
ANSWER TWO OF THE THREE QUESTIONS
10 POINTS EACH
41.
DESCRIBE FIVE MAJOR DIFFERENCES BETWEEN BACTERIAL CELLS AND
VIRUSES (INCLUDE BOTH STRUCTURAL AND LIFE CYCLE DIFFERENCES).
42.
DESCRIBE THE DIFFERENCE BETWEEN A VIROID AND A PRION. WHAT TYPE
OF ORGANISM DOES EACH OF THESE AGENTS INFECT AND WHAT GENERAL
TYPE OF DISEASE IS PRODUCED?
43.
DRAW DIAGRAMS OF BOTH IgG AND IgA. LABEL BOTH THE HEAVY CHAINS
AND THE LIGHT CHAINS AND LOCATE THE ANTIGEN BINDING SITES.