NAME_______________________________
SSN_____________________
MCB 2004
MICROBIOLOGY
SAMPLE TEST
IN THIS PART THERE ARE FORTY QUESTIONS WORTH TWO POINTS EACH. THERE
IS ONLY ONE CORRECT ANSWER FOR EACH QUESTION.
PLEASE CIRCLE THE
CORRECT LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF THE
QUESTION NUMBER.
THEN TRANSFER ALL YOUR ANSWERS TO THE "ACCU-SCAN"
ANSWER SHEET.
KEY FOR QUESTIONS 1-7. THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY
WORD MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
MITOCHONDRIA
CHLOROPLASTS
NUCLEI
PILI
PLASMA MEMBRANES
_____1.
THE PRODUCTION OF ATP FROM THE ENERGY OF LIGHT OCCURS HERE
_____2.
FOUND IN BOTH PROKARYOTES AND EUKARYOTES
_____3.
IN PROKARYOTES IT IS THE SITE OF THE ELECTRON TRANSPORT
CHAIN
_____4.
HERE RESIDES A DARK-STAINING BODY CALLED THE NUCLEOLUS
_____5.
IN EUKARYOTIC CHEMOHETEROTROPHS THIS IS WHERE MOST ATP IS
MADE
6.
IN THE GRAM STAIN THE MORDANT IS _________.
A.
BASIC FUCHSIN
B.
IODINE
C.
CRYSTAL VIOLET
D.
WATER
E.
ACETONE OR ALCOHOL
7.
STAINING FIXED CELLS ONLY WITH METHYLENE BLUE IS AN EXAMPLE OF:
A.
SPECIAL STAINING
B.
SIMPLE STAINING
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
MCB 2004
TEST #1
PAGE 2
8.
IN BRIGHT FIELD MICROSCOPY LIGHT PASSING THROUGH THE
SPECIMEN (ON THE STAGE) NEXT PASSES THROUGH THE
A.
OBJECTIVE LENS
B.
LIGHT SOURCE
C.
CONDENSER
D.
OCULAR LENS
9.
THE ACID FAST OUTER LIPID LAYER IS UNUSUALLY THICK BECAUSE OF
A.
MYCOLIC ACIDS
B.
TEICHOIC ACIDS
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
10.
MICROORGANISMS THAT GROW IN AND AROUND THE HOT SPRINGS OF
YELLOWSTONE ARE PROBABLY
A.
HALOPHILES
B.
THERMOPHILES
C.
MICROAEROPHILES
D.
MESOPHILES
E.
PSYCHROPHILES
11.
THE DOUBLING TIME OF E. COLI IN NUTRIENT BROTH AT 37o C IS 30
MINUTES. THE INITIAL INOCULUM OF THE CULTURE CONTAINS 15 CELLS.
AFTER TWO HOURS OF EXPONENTIAL GROWTH THE CULTURE CONTAINS
A.
60 CELLS
B.
120 CELLS
C.
150 CELLS
D.
240 CELLS
E.
400 CELLS
12.
A BACTERIUM THAT OXIDIZES SULFUR WITH OXYGEN FOR ENERGY AND
USES CARBON DIOXIDE AS ITS CARBON SOURCE IS A
A.
CHEMOAUTOTROPH
B.
STRICT ANAEROBE
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
13.
THE FOLLOWING IS ONE INGREDIENT OF A DEFINED MEDIUM
A.
NUTRIENT AGAR
B.
GLUCOSE
C.
PEPTONE
D.
YEAST EXTRACT
E.
BEEF INFUSION
MCB 2004
TEST #1
PAGE 3
14.
THEIR DISCOVERY IN 1953 EARNED THEM THE NOBEL PRIZE AND MARKED
THE BEGINNING OF THE AGE OF MOLECULAR BIOLOGY.
A.
KOCH & PASTEUR
B.
WATSON & CRICK
C.
VAN LEEUWENHOEK & HOOK
D.
LOEFFLER & FROSH
E.
MASTERS & JOHNSON
15.
HIS OBSERVATION THAT MILKMAIDS WERE IMMUNE TO SMALLPOX LED TO
THE FIRST USEFUL VACCINE
A.
JONAS SALK
B.
LOUIS PASTEUR
C.
ALEXANDER FLEMING
D.
PAUL EHRLICH
E.
EDWARD JENNER
16.
PEPTIDOGLYCAN IS MADE OF POLYMERS OF TWO SUGARS CROSSED-LINKED BY
A.
LIPIDS
B.
PEPTIDE CHAINS
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
17.
LIPOPOLYSACCHARIDE (LPS) IS DIVIDED INTO THREE SECTIONS WHICH
INCLUDE
A.
THE CORE POLYSACCHARIDE
B.
THE O ANTIGEN
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
18.
THE LIGHT MICROSCOPE
MAGNIFICATION.
A.
0.4 mm,
10
B.
10 µm,
100
C.
0.2 µm,
2,000
D.
2 nm , 100,000
19.
IS CAPABLE OF ____ RESOLUTION, AND ____
X
X
X
X
ONE METER EQUALS
A.
1000 mm
B.
1,000,000 µm
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
MCB 2004
TEST #1
PAGE 4
20.
THE DARKFIELD MICROSCOPE IS USEFUL FOR
A.
SEEING FLAGELLA
B.
DIAGNOSING SYPHILIS
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
21.
THE NUMBER OF BACTERIA PER ML IN THIS CULTURE FLASK IS
A.
42,000
B.
210,000
C.
420,000
D.
2,100,000
1.0 ml
1.0 ml
1.0 ml
1.0 ml
E.
4,200,000
9.0 ml
9.0 ml
9.0 ml
42 COLONIES
KEY FOR QUESTIONS 23-30. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
ESCHERICHIA COLI
B.
BACILLUS SUBTILIS
C.
MYCOBACTERIUM LEPRAE
D.
TREPONEMA PALLIDUM
E.
CHLAMYDIA TRACHOMATIS
_____22.
ITS "LIFE CYCLE" ALTERNATES BETWEEN ELEMENTARY BODIES AND
RETICULATE BODIES
_____23.
CONTAINS LIPOPOLYSACCHARIDE (LPS)
_____24.
STAINS RED IN THE ACID FAST STAIN
_____25.
IT MOVES RAPIDLY BECAUSE OF ITS AXIAL FILAMENTS
_____26.
AFTER THE GRAM STAIN YOU SEE PURPLE RODS AT 1000x
MCB 2004
TEST #1
PAGE 5
27.
KILLING UNDESIRABLE ORGANISMS BY MOIST HEAT (63OC, 30 MINUTES)
IS KNOWN AS
A.
FILTRATION
B.
INCINERATION
C.
STERILIZATION
D.
LYOPHILIZATION
E.
PASTEURIZATION
28.
MANY ANTIBIOTICS AND VITAMINS WHICH ARE UNSTABLE TO HEAT ARE
ADDED TO BACTERIAL GROWTH MEDIA AFTER THEY ARE
A.
AUTOCLAVED
B.
PASTEURIZED
C.
FROZEN
D.
FILTERED
E.
BOILED
29.
IF YOU WANTED TO STERILIZE PLASTIC-WARE (LIKE YOUR PLASTIC
PETRI DISHES OR DISPOSABLE PIPETS) YOU COULD USE
A.
AUTOCLAVING
B.
ETHYLENE OXIDE
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
30.
EUKARYOTIC CELLS ARRESTED JUST BEFORE MITOSIS ARE IN
A.
Go
B.
G1
C.
G2
D.
M
E.
S
31.
STAPHYLOCOCCUS AUREUS GROWS AS
A.
LONG THIN RODS
B.
CHAINS OF RODS
C.
CLUSTERS OF SPHERICAL CELLS
D.
CHAINS OF SPHERICAL CELLS
E.
PAIRS OF SPHERICAL CELLS
32.
IN THIS PROCESS IN E. COLI, ONE DOUBLE STRANDED CIRCLE OF DNA
IS CONVERTED TO TWO DOUBLE STRANDED CIRCLES OF DNA
A.
TRANSCRIPTION
B.
FEED BACK INHIBITION
C.
TRANSLATION
D.
REPLICATION
E.
REPRESSION
MCB 2004
TEST #1
PAGE 6
33.
IN THIS PROCESS, mRNA, RIBOSOMES, and TRANSFER RNA ARE ALL
REQUIRED
A.
TRANSCRIPTION
B.
FEED BACK INHIBITION
C.
TRANSLATION
D.
REPLICATION
E.
REPRESSION
34.
IN THIS PROCESS THE FIRST ENZYME OF A ANABOLIC PATHWAY IS
REGULATED BY THE FINAL PRODUCT OF THE PATHWAY
A.
TRANSCRIPTION
B.
FEED BACK INHIBITION
C.
TRANSLATION
D.
REPLICATION
E.
REPRESSION
35.
COMPONENTS OF THE LACTOSE OPERON INCLUDE:
A.
AN OPERATOR SITE
B.
THE Z (-GALACTOSIDASE) GENE
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
KEY FOR QUESTIONS 36-40. EACH QUESTION HAS ONLY ONE ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
THE KERBS (TCA) CYCLE
PROTEIN SYNTHESIS
LACTIC ACID FERMENTATION
THE ELECTRON TRANSPORT CHAIN
HISTIDINE BIOSYNTHETIC PATHWAY
_____36.
ANABOLISM OF AN AMINO ACID
_____37.
IN THIS PROCESS ARE PRODUCED THREE NADH, ONE FADH2 AND ONE
ATP (ALSO ACETATE IS OXIDIZED TO 2 CO2)
_____38.
ANABOLISM OF A MACROMOLECULE
_____39.
THE OXIDATION OF NADH OR FADH2 BY MOLECULAR OXYGEN
_____40.
ONLY TWO ATPS ARE PRODUCED FROM ONE GLUCOSE IN THIS PROCESS
MCB 2004
TEST #1
PAGE 7
ANSWER TWO OF THE FOLLOWING THREE QUESTIONS (10 POINTS EACH)
41.
DRAW A TYPICAL BACTERIAL GROWTH CURVE AND LABEL IT (DON'T
FORGET TO LABEL THE AXES.).
42.
DRAW A DIAGRAM OF A GRAM NEGATIVE CELL ENVELOPE AND LABEL IT.
NAME ONE GRAM NEGATIVE BACTERIUM (GENUS AND SPECIES).
43.
EXPLAIN THE DIFFERENCE BETWEEN A PHOTOAUTOTROPH AND A
CHEMOHETEROTROPH. GIVE AN EXAMPLE OF (OR NAME OF) ONE ORGANISM
OF EACH TYPE (DOESN'T HAVE TO BE A BACTERIUM).