1.An Introduction to the Spectrophotometer 2. Use of the Spectrophotometer 3. Tutorial of the Spectrophotometer 4. The Spectrophotometer_ A demo and practice. For students to learn basic concepts of measurement using the spectrophotometer A spectrophotometer is a photometer that can measure intensity as a function of the light source wavelength. It consists of two parts spectrometer for producing light of any selected color (wavelength) photometer for measuring the intensity of light. Uses a type of light to detect molecules in a solution Light is a type of energy, and the energy is reported as wavelengths, in nanometers (nm). Ultraviolet (UV) Spectrophotometers. Uses ultraviolet light of wave lengths from 200 nm to 380 nm. Visible (VIS) Light Spectrum Spectrophotometers. Uses visible light (white light) of wave lengths from 380 nm to 720 nm. If the cuvettes are not clean and dry, rinse them thoroughly with the solution that you will be measuring. Rinsing several times with small volumes of the solution is preferable to rinsing once with a large volume of the solution. Filled properly. Not filled enough. Shines The a beam of light on a sample. molecules in the sample interact with the light waves in of 3 ways: The spectrophotometer measures the amount of light transmitted through the sample (Transmittance). By using an equation (Beers law), it converts the transmittance data to an absorbance value. What kind of data is this? Turn the spectrophotometer on at least 10-15 minutes before using. Set the wavelength desired. With the chamber empty and closed, adjust the machine to read 0%. Determines the presence and concentrations of samples. Determines the purity of a sample. Look at the change of samples over time.