1.An Introduction to the Spectrophotometer
2. Use of the Spectrophotometer
3. Tutorial of the Spectrophotometer
4. The Spectrophotometer_ A demo and practice.
 For
students to learn basic concepts of
measurement using the spectrophotometer
A spectrophotometer is a photometer
that can measure intensity as a function
of the light source wavelength.
It consists of two parts
spectrometer
for producing light of any
selected color (wavelength)
photometer
for measuring the intensity of
light.
 Uses
a type of light to detect
molecules in a solution
 Light
is a type of energy, and the
energy is reported as wavelengths,
in nanometers (nm).
 Ultraviolet
(UV) Spectrophotometers.
 Uses ultraviolet light of wave lengths
from 200 nm to 380 nm.
 Visible
(VIS) Light Spectrum
Spectrophotometers.
 Uses visible light (white light) of wave
lengths from 380 nm to 720 nm.
If the cuvettes are not
clean and dry, rinse
them thoroughly with
the solution that you
will be measuring.
Rinsing several times
with small volumes of
the
solution
is
preferable to rinsing
once with a large
volume
of
the
solution.
Filled properly.
Not filled enough.
Shines
The
a beam of light on a sample.
molecules in the sample interact with
the light waves in of 3 ways:
The
spectrophotometer measures the
amount of light transmitted through the
sample (Transmittance).
By
using an equation (Beers law), it
converts the transmittance data to an
absorbance value.
What kind of data is this?
Turn
the spectrophotometer on at
least 10-15 minutes before using.
Set
the wavelength desired.
With
the chamber empty and closed,
adjust the machine to read 0%.
Determines
the presence and
concentrations of samples.
Determines the purity of a
sample.
Look at the change of samples
over time.