OXACILLIN AND POLIMIXIN B SELECTIVE SUPPLEMENT
CAT Nº:6068
Selective supplement isolation of methicillin resistant
Staphylococcus aureus (MRSA) directly from routine swab samples
FORMULA PER VIAL (Each vial for 500 ml of medium)
Oxacillin
1.00 mg
Polymixin B
25.000 UI
PREPARATION
Aseptically reconstitute 1 vial with 5 ml of sterile distilled water. Mix gently until complete dissolution.
Aseptically add to 500 ml of OXACILLIN AGAR BASE (Cat. 1196), autoclaved and cooled to 50ºC. Mix well
and distribute into sterile containers.
The antibiotics contained in Cat. 6068 Oxicillin and polimyxin B inhibit methicillin sensitive Staphylococcus
aureus (MSSA) and polymyxin B for the suppression of other bacteria able to grow at such a high salt
concentration, e.g. Proteus spp.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 37 ± 2°C and observed after 24– 48 hours.
Growth
Microorganisms
Staphylococcus aureus ATCC
25923
Staphylococcus aureus ATCC
43300
Escherichia coli ATCC 25922
Inhibited (susceptible to
Oxacillin)
Good (presence of
colony or haze
of growth
should be read
as resistance)
Inhibited
Colony
Blue
BIBLIOGRAPHY
Hutchison, M.J., Edwards, G.F.S., Morrison, D. bEvaluation of chromogenic MRSA Reference Laboratory presented at the 2005 Institute
of BioMedical
Jablonski, L.M. and G.A. Bohach. 1997. Staphylococcus aureus. In M. Doyle, L. Beuchat and T. Montville (eds.), Food microbiology
fundamentals and frontiers. ASM, Washington, DC.
U.S. Department of Health and Human Services. 1999. Biosafety in microbiological and biomedical laboratories, HHS Publication (CDC),
4th ed. U.S. Government Printing Office, Washington, DC.
STORAGE
When stored as indicated, supplements remain stable until the stated expiry date shown on the label.
8ºC
2ºC
1
LABORATORIOS CONDA, S.A.
www.condalab.com