SABOURAUD DEXTROSE AGAR + CYCLOHEXIMIDE
CAT Nº: 1088
For the selective cultivation of yeasts and molds
FORMULA IN g/l
Dextrose
40.00
Cycloheximide
0.40
Peptone Mixture
10.00
Bacteriological Agar
15.00
Final pH 5.6 ± 0.2 at 25ºC
PREPARATION
Suspend 65.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent
agitation. Boil for one minute until complete dissolution. Distribute and sterilize in autoclave at 118-121ºC for 15
minutes. AVOID OVEARHEATING as it facilitates the hydrolysis of the components and the medium remains soft. The
prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.
Caution: This medium is toxic if swallowed, inhaled or comes into contact with skin. Wear gloves and eye/face protection.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium
USES
SABOURAUD DEXTROSE AGAR + CYCLOHEXIMIDE is a selective medium that can be used for cultivating yeasts, molds
and aciduric microorganisms. It is also used for cultivating pathogenic fungi, particularly those associated with skin
infections. This medium is at the same time used for determining the microbial and fungal content of cosmetics and for
the mycological evaluation of food.
D e x tro s e i s t h e c ar b oh y dr a t e e n er g y s ourc e; P e p to n e m i xt ur e i s th e n it rog e n, v i t am in s a n d am ino
a c i ds sou rc e; an d t h e Bacteriological agar is the solidifying agent. The high dextrose concentration
and acidic pH make this medium selective for fungi.
This medium is a modification of the Dextrose Agar described by Sabouraud, with the addition of Chloramphenicol and
Cycloheximide. Cycloheximide is an antibiotic which inhibits saprophytic fungi but allows for the growth of pathogenic
fungi: Cryptococcus neoformans, Aspergillus fumigatus and some species of Candida (albicans, krusei).
Inoculate sample and incubate at 30°C during 3 days and up yo 7 days if necessary..
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 30°C and observed after 3 - 7 days.
Microorganisms
Candida albicans ATCC 2091
Escherichia coli ATCC 25922
Aspergillus brasiliensis ATCC 16404
Penicillium spp.
Trychophyton mentagrophytes
BIBLIOGRAPHY
LABORATORIOS CONDA, S.A.
Growth
Good
Good/Moderate
Inhibited/Light
Inhibited/Light
Good
1
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M.R. Pascual Anderson (1982) Técnicas para Análisis Microbiológico de Alimentos y Bebidas.
Sabouraud R. 1892. Ann. Dermatol. Syphilol. 3:1061.
Jarett, L., and A.C. Sonnenwirth (ed) 1980. Gradwohl’s clinical laboratory methods and diagnosis, 8th ed. CV Mosby.
Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (ed) 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance
Association, Washington, D.C.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com