CAT Nº: 1134
Dextrose 40.00 Chloramphenicol 0.05
Peptone (Meat & Casein) 10.00 Bacteriological Agar 15.00
Suspend 65 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. Distribute and sterilize in autoclave at 118-121ºC for 15 minutes. AVOID
OVEARHEATING as it facilitates the hydrolysis of components and the medium remains soft. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.
SABOURAUD DEXTROSE AGAR + CHLORAMPHENICOL is a selective medium that can be used for the cultivation of yeasts, molds and aciduric microorganisms. It is used for cultivating pathogenic fungi, particularly those associated with skin infections. This medium is also used for determining the microbial and fungal content of cosmetics and for the mycological evaluation of food.
The formula is based on the European Pharmacopoeia. Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent. The high dextrose concentration and acidic pH make this medium selective for fungi.
This medium is a modification of the Dextrose Agar described by Sabouraud, with the addition of Chloramphenicol that inhibits a great majority of bacterial contaminants.
Chloramphenicol is an antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits most contaminating bacteria. It is a recommended antibiotic for use with media due to its heat stability and wide bacterial spectrum.
This medium has a lower concentration of Chloramphenicol than the medium of the same name (Cat. 1090), making it less inhibitory to contaminant bacteria.
The European Pharmacopoeia recommends subculturing positive fungal strains separately to the medium at 20 – 25°C for 48 hours for
and at 20 – 25°C for 7 days for
.
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 30°C and observed after 3 - 7 days.
Microorganisms Growth
Inoculum Recovery
Rate %
1
LABORATORIOS CONDA, S.A.

* Candida albicans ATCC 10231
* * Aspergillus brasiliensis ATCC 16404
Escherichia coli ATCC 25922
Good
Good
Inhibited
Inhibited
10
2
-10
3
10
2
-10
3
10
2
-10
3
10
2
-10
3 Staphylococcus aureus ATCC 25923
* According to European Pharmacopeia incubate at 20-25ºC for 48 hours
* * According to European Pharmacopeia incubate at 20-25ºC for 7 days
≥ 70
≥ 70
≤ 0.01
≤ 0.01
Sabouraud R. 1892. Ann. Dermatol. Syphilol. 3:1061.
Jarett, L., and A.C. Sonnenwirth (ed) 1980. Gradwohl’s clinical laboratory methods and diagnosis, 8th ed. CV Mosby.
Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (ed) 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance
Association, Washington, D.C:
European Pharmacopoeia. 6th Edition. 2007
EP
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
LABORATORIOS CONDA, S.A.
2