MYCOBIOTIC AGAR (FUNGAL SELECTIVE AGAR)
CAT Nº: 1072
For the isolation of fungal pathogens in highly contaminated samples
FORMULA IN g/l
Soy Peptone
10.00
Chloramphenicol
0.05
Dextrose
10.00
Bacteriological Agar
15.50
Cycloheximide
0.40
Final pH 6.9 ± 0.2 at 25ºC
Candida albicans
ATCC 2091
PREPARATION
Suspend 36 grams of the medium in one liter of distilled water. Mix well. Dissolve by heating with frequent agitation. Boil
for one minute until complete dissolution. Sterilize in autoclave at 118ºC for 15 minutes. Cool to 50ºC, mix well and
dispense into plates. Use it immediately. Once cold, remelt only once with the minimum heat. DO NOT OVEARHEAT. The
prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
Caution: This medium is toxic if swallowed, inhaled or comes into contact with skin. Wear gloves and eye/face protection.
USES
MYCOBIOTIC AGAR is a medium for the selective cultivation of fungal pathogens from diverse clinical samples and other
materials contaminated with a mixed associated flora.
This medium is basically a Mycology Agar to which Chloramphenicol has been added to inhibit bacterial development,
and Cycloheximide to inhibit the growth of saprophytic fungi. Mycobiotic Agar is very useful in isolating pathogenic fungi
from diverse types of highly contaminated samples with different types of accompanying flora, such as those of the
head, skin scrapings, nails, bronchial lavages, gastric juices, soil, etc.
Soy peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable
carbohydrate as a carbon and energy source. Bacteriological agar is the solidifying agent. Chloramphenicol is an
antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits most contaminating
bacteria. It is a recommended antibiotic for use with media due to its heat stability and wide bacterial spectrum.
Cycloheximide is an antibiotic which inhibits saprophytic fungi but allows for growth of the pathogenic fungi.
It is recommended to inoculate several plates or tubes with the same sample in study and incubate them at 22 - 25°C
and at 35°C. The toxic effect of the antimicrobial mixture is greater at 20 - 25°C, reason for which the number of
positive isolates is higher at temperatures below 35°C.
It is recommended to inoculate other culture media at the same time such as Biggy Agar (Cat. 1006) with the objective
to obtain a greater number of isolates. The dermatophytes and other numerous groups of pathogenic fungi grow quickly
in the Mycobiotic Agar as it inhibits most of the bacteria and fungal saprophytes or commensal contaminants.
MICROBIOLOGICAL TEST
1
LABORATORIOS CONDA, S.A.
www.condalab.com
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 30°C and observed after 3 - 7 days.
Microorganisms
Growth
Escherichia coli ATCC 25922
Staphylococcus aureus ATCC 25923
Trichophyton mentagrophytes ATCC 9533
Trichophyton rubrum ATCC 28188
Candida albicans ATCC 2091
Aspergillus brasiliensis ATCC 16404
Penicillium spp.
Inhibited
Inhibited
Good
Good
Good
Inhibited
Inhibited
BIBLIOGRAPHY
Dean and Halley, Public Health Reports, 77:61. 1972. Hupper and Walker, A.J. Clin. Path. 29:291. 1958. McDonough Ajello, Georg, and
Brinkman J. Lab. and Clin. Med. 55:116. 1960.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com