SABOURAUD DEXTROSE BROTH + CHLORAMPHENICOL
CAT Nº: 1284
For the selective cultivation and isolation of yeasts and molds
FORMULA IN g/l
Dextrose
20.00
Peptone Mixture
10.00
Chloramphenicol
0.5
Final pH 5.6 ± 0.2 at 25ºC
PREPARATION
Suspend 30.5 grams of the medium in one liter of distilled water. .Mix well and dissolve by heating with frequent
agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at
121°C for 15 minutes, DO NOT OVERHEAT. Store at 2-8°C. The color of the prepared medium is amber, slightly
opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
SABOURAUD DEXTROSE BROTH + CHLORAMPHENICOL is a selective medium that can be used for the cultivation of
yeast, molds and aciduric microorganisms. It is used for cultivating pathogenic fungi, particularly those associated with
skin infections. This medium is also used for determining the microbial and fungal content of cosmetics and for the
mycological evaluation of food.
Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone mixture provides nitrogen, vitamins,
minerals and amino acids essential for growth. The dextrose concentration and acidic pH make this medium selective for
fungi.
This medium is a modification of the Dextrose Agar described by Sabouraud, with the addition of Chloramphenicol,
which inhibits the great majority of bacterial contaminants and in broth.
Chloramphenicol is an antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits
most contaminating bacteria. It is a recommended antibiotic for use with media due to its heat stability and wide
bacterial spectrum.
The incubation of the plates should be at 30°C for 3 – 7 days.
This medium has a higher concentration of Chloramphenicol than the medium of the same name (Cat. 1134), and, as
such, is more inhibiting to contaminant bacteria.
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a
temperature of 30 ± 2°C and observed after 3 - 7 days.
Microorganisms
Growth
Candida albicans ATCC 2091
Candida tropicalis ATCC 750
Escherichia coli ATCC 25922
Staphylococcus aureus ATCC 25923
Good
Good
Inhibited
Inhibited
1
LABORATORIOS CONDA, S.A.
www.condalab.com
BIBLIOGRAPHY
Sabouraud R. 1892. Ann. Dermatol. Syphilol. 3:1061.
Jarett, L., and A.C. Sonnenwirth (ed) 1980. Gradwohl’s clinical laboratory methods and diagnosis, 8th ed. CV Mosby.
Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (ed) 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance
Association, Washington, D.C.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com