SABOURAUD MALTOSE AGAR
CAT Nº: 1054
For the cultivation of yeasts and molds
FORMULA IN g/l
Maltose
40.00
Peptone Mixture
10.00
Bacteriological Agar
15.00
Final pH 5.6 ± 0.2 at 25ºC
PREPARATION
Suspend 65 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. Sterilize in autoclave at 118-121ºC for 15 minutes. Cool to 45-50ºC, mix
well and dispense into plates. AVOID OVERHEATING as it facilitates the hydrolysis of the components and the medium
remains soft. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
SABOURAUD MALTOSE AGAR is a modification of Sabouraud Dextrose Agar with maltose substituting dextrose. It is a
selective medium due to its acidic pH. Davidson, Dawding and Buller reported that Sabouraud Maltose Agar was a
satisfactory medium for isolating Trichophyton gypseum from a case of Tinea barbae and in their studies of the
infections caused by Microsporum audouinii and Trichophyton gypseum.
Maltose is the fermentable carbohydrate providing carbon and energy. Peptone mixture provides nitrogen, vitamins,
minerals and amino acids essential for growth. The low pH suits fungi growth, particularly dermatophytes, and is slightly
inhibitory to contaminating bacteria in clinical specimens. Bacteriological agar is the solidifying agent.
Georg et al. demonstrated that basic agar fortified by three antibiotics considerably improves the isolation of pathogenic
fungi from heavily contaminated sources.
To prepare a selective culture medium aseptically add the following for every ml of the medium before use: Penicillin +
Streptomycin or Chloramphenicol or Neomycin. Inoculate sample and incubate at 30°C for up to 7 days.
MICROBIOLOGICAL TEST
The following results were obtained from type cultures in the performance of the medium after incubation at a
temperature of 30°C and observed after 7 days.
Microorganisms
Growth
Aspergillus brasiliensis ATCC 16404
Good
Candida albicans ATCC 26790
Good
Trichophyton mentagrophytes ATCC 9533
Good
Escherichia coli ATCC 25922
Lactobacillus casei ATCC 9595
Partially Inhibited
Good
1
LABORATORIOS CONDA, S.A.
www.condalab.com
BIBLIOGRAPHY
McDonough, Ajello, Georg, and Brinkwan J. Lab and Clin. Med. S.S. 1960. Chapman, G. H. The Isolation and Differentation of Monilia
and Other Fungi, Trans. New York Sc. Series II 14(6), 154 (1952).
Chapman G. H. (1 952). Trans. New York Acad. Sci., Series II 1 4(6).254.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com