Version 2 Universidade Nacional da Irlanda em Galway -- Ciência sem Fronteiras PhD Project Template Use one form per project Please complete & submit to international@nuigalway.ie as soon as possible, and by 27/11/2012 In your email, begin the subject line with [SWB] (be sure to use square brackets) to ensure that your email is filed correctly. Emails will be automatically filed PI name & contact details: Dr Conor P O’Byrne School: School of Natural Sciences Has project been agreed with head (or nominee) of proposed registration school? Research Centre / group affiliation: Microbiology Research group / centre website: Bacterial Stress Response Group PI website / link to CV: http://www.nuigalway.ie/microbiology/cpoblab/ Brief summary of PI research / research group / centre activity (2 or 3 lines max): If you are already involved in relevant scholarly activities relating to Brazil, please mention them here. Research activity within the group focuses on the mechanisms that food-borne bacterial pathogens use to respond and adapt to environmental perturbations. An understanding of how these pathogens adapt to their environment will also be central to any strategy aimed at curbing their growth both within the host and within the food-chain. Title & brief description of PhD project (suitable for publication on web): Molecular mechanisms of stress sensing in the food-borne bacterial pathogen Listeria monocytogenes. Listeria monocytogenes is a food-borne bacterial pathogen that causes lifethreatening illness in humans. It is a highly adaptable pathogen and can survive harsh food preservation regimes and also withstand the stresses encountered in the gastrointestinal tract during the early stages of an infection. The remarkable ability to tolerate stress is controlled to a large extent by a transcription factor called sigma B. In this project we will use molecular genetic tools to investigate the way in which Listeria monocytogenes senses stress and activates this transcription factor. We will focus attention on the proteins believed to comprise the sensory apparatus . Mutagenesis and careful phenotypic studies will be used to identify and characterise the components that are required for sensing each of the key stresses that Listeria encounters in the food chain and within the host (pH, temperature, bile and changing osmolarity). Key words: Bacteria, Listeria, pathogens, virulence, pathogenesis, gene transcription, sigma factor, food-borne disease, molecular biology, stress response, gene regulation. Version 2 Universidade Nacional da Irlanda em Galway -- Ciência sem Fronteiras Unique selling points of PhD project in NUI Galway: The student working on this project will join an established group of researchers with existing expertise in this area. The group has established a strong publication record in this field and the student will be encouraged to publish their own findings. The group is based in Microbiology within the School of Natural Sciences, which is has excellent shared facilities for molecular biology including walk-in culture rooms, facilities for gel processing and imaging, microscopy, genomics, real time PCR, proteomics, protein purification. Galway combines a student-centred environment with world-leading research groups to create a highly stimulating environment for post-graduate students. The PhD programme within Microbiology will include options to take relevant course work in parallel with the main research project. Topics such as scientific writing, statistical methods, presentation skills, advanced microscopy, infection and immunity are all included as options. Name & contact details for project queries, if different from PI named above: Please indicate the graduates of which disciplines that should apply: Biological science disciplines, including biochemistry, microbiology, genetics, molecular biology or other related disciplines. Ciência sem Fronteiras / Science Without Borders Priority Area: Please indicate the specific programme priority area under which the proposed PhD project fits- choose only one (tick box): Engineering and other technological areas Pure and Natural Sciences (e.g. mathematics, physics, chemistry)/Physical Sciences (Mathematics, Physics, Chemistry, Biology and Geosciences) Health and Biomedical Sciences / Clinical, Pré-clinical and Health Sciences Information and Communication Technologies (ICTs), Computing Aerospace Pharmaceuticals Sustainable Agricultural Production Oil, Gas and Coal Renewable Energy Minerals, Minerals Technology Biotechnology Nanotechnology and New Materials Technologies for Prevention and Mitigation of Natural Disasters Bioprospecting and Biodiversity Marine Sciences Creative Industry New technologies in constructive engineering Version 2 Universidade Nacional da Irlanda em Galway -- Ciência sem Fronteiras Please indicate which of the following applies to this project (referring to Science Without Borders arrangements): Suitable only as a Full PhD (Y/N): _ Y____ Available to candidates seeking a Sandwich PhD arrangement (Y/N): __N___ Suitable for either/Don’t know: _____