Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
Anti-Choline Acetyltransferase antibody
Chicken polyclonal to Choline Acetyltransferase
IHC-P, ICC
Reacts with: Mouse, Rat, Human
A synthetic peptide,conjugated to KLH, corresponding to a region of homology shared by Mouse
(Q03059) and Human (P28329) Choline Acetyltransferase.
Adult mouse brain tissue.
Properties
Form
Storage instructions
Storage buffer
Purity
Purification notes
Clonality
Isotype
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Immunogen affinity purified
IgY fractions were purified from the yolks laid by immunized Chickens
Polyclonal
IgY
Applications
Our Abpromise guarantee covers the use of ab134021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/1000 - 1/2000.
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Target
Function
Involvement in disease
Sequence similarities
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea
(CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2).
CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
Belongs to the carnitine/choline acetyltransferase family.
Anti-Choline Acetyltransferase antibody images
Immunohistochemical analysis of a section of adult mouse brain labelling Choline
Acetyltransferase (red staining) using ab134021 at 1/1000 dilution in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of green fluorescent protein (GFP) in this transgenic animal.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline
Acetyltransferase antibody (ab134021)
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