Product datasheet
Anti-Choline Acetyltransferase antibody ab34419
1 References 1 Image
Overview
Product name
Anti-Choline Acetyltransferase antibody
Description
Chicken polyclonal to Choline Acetyltransferase
Tested applications
WB, ICC/IF, IHC-Fr, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (Mouse)
Positive control
Mouse/rat brain.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.2
Purity
Protein L purified
Clonality
Polyclonal
Isotype
IgY
Applications
Our Abpromise guarantee covers the use of ab34419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/250 - 1/500. Predicted molecular weight: 83 kDa.
ICC/IF
1/100 - 1/200.
IHC-Fr
1/50.
IHC-P
Use at an assay dependent dilution.
Target
1
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at
cholinergic synapses.
Involvement in disease
Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea
(CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2).
CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic
symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt
episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue
exertion, fever, or excitement.
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family.
Anti-Choline Acetyltransferase antibody images
This image shows a section of a formalinfixed paraffin-embedded adult mouse brain
showing ChAT (red staining) in cholinergic
neurons of the caudate-putamen nucleus.
Antibody ab34419 at a dilution of 1/100.
Immunocytochemistry/ Immunofluorescence Choline Acetyltransferase antibody (ab34419)
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