Product datasheet
Anti-Choline Acetyltransferase antibody ab6168
7 Abreviews 3 References 2 Images
Overview
Product name
Anti-Choline Acetyltransferase antibody
Description
Rabbit polyclonal to Choline Acetyltransferase
Specificity
Extensive immunohistochemistry indicates localisation to cholinergic nervous system only. Cross
reactivity: This antibody is known to react with rat and guinea pig. Cross reactivity with other
species has not been tested yet.
Tested applications
ICC/IF, ELISA, IHC-P, IHC-Fr
Species reactivity
Reacts with: Mouse, Rat, Guinea pig
Predicted to work with: Chicken, Human, Non Human Primates
Does not react with:
Sheep
Immunogen
Synthetic peptide:
H-GLFSSYRLPGHTQDTLVAQKSS
conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 168-189 of Choline
Acetyltransferase.
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General notes
Run BLAST with
This antibody appears to have identical characteristics to those described by Benecke et al
(1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both
peripheral and central nervous system, staining both cell bodies and nerve terminals.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for
extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab6168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
ICC/IF
Use at an assay dependent concentration. See Abreview.
ELISA
1/1000.
IHC-P
Use at an assay dependent concentration. PubMed: 19422789
IHC-Fr
1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and
post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde
(4% formaldehyde with or without 15% picric acid is a very good fixative, it should
also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in
30% sucrose (at +4C). Use on free floating sections for best results.
This antibody does not work with glutaraldehyde fixation.
Target
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at
cholinergic synapses.
Involvement in disease
Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea
(CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2).
CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic
symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt
episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue
exertion, fever, or excitement.
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family.
Anti-Choline Acetyltransferase antibody images
ab6168 staining rat stomach tissue sections
by immunohistochemistry (Frozen sections).
Tissue underwent fixation in
paraformaldehyde. The primary antibody was
diluted 1/200 and incubated with sample
for 24 hours. A Texas Red® conjugated goat
polyclonal (IgG whole molecule) to rabbit IgG,
diluted 1/400 was used as secondary.
Immunohistochemistry (Frozen sections) Choline Acetyltransferase antibody - Neuronal
Marker (ab6168)
Marcin Arciszewski, Org unknown, Country unknown
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ab6168 staining cells in rat brain section by
ICC/IF. Cells were PFA fixed and blocked in
10% serum for 1 hour at 25°C. The primary
antibody was diluted 1/200 in 10% donkey
serum and incubated with the sample for 24
hours at 25°C. A FITC-conjugated donkey
polyclonal to rabbit IgG, diluted 1/200 was
used the secondary.
Immunocytochemistry/ Immunofluorescence Choline Acetyltransferase antibody - Neuronal
Marker (ab6168)
This image is courtesy of an Abreview submitted by
Dr Li-Hsien Lin
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