Product datasheet
Anti-Choline Acetyltransferase antibody ab18736
2 Abreviews 7 References 2 Images
Overview
Product name
Anti-Choline Acetyltransferase antibody
Description
Sheep polyclonal to Choline Acetyltransferase
Tested applications
IHC-FoFr, IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human, Pig
Predicted to work with: Chicken
Immunogen
Synthetic peptide:
GLFSSYRLPGHTQDTLVAQKSS
conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase.
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for
extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab18736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-FoFr
Abreviews
Notes
1/1000.
Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min
then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for
20min). Please note sections must be post fixed if the tissue is cut freshly.
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Application
Abreviews
Notes
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
ICC/IF
1/100. PubMed: 18393628
Target
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at
cholinergic synapses.
Involvement in disease
Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea
(CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2).
CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic
symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt
episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue
exertion, fever, or excitement.
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family.
Anti-Choline Acetyltransferase antibody images
ab18736 staining Choline Acetyltransferase
in mouse brain tissue sections by
Immunohistochemistry (PFA perfusion fixed
frozen sections). Tissue samples were fixed
by perfusion with paraformaldehyde,
permeablized with 0.3% Triton X-100
and blocked with 3% donkey serum in 0.3%
Triton X-100/TBS for 1 hour at 21°C. The
sample was incubated with primary antibody
Immunohistochemistry (PFA perfusion fixed
(1/1000 in 1% donkey serum + 0.3% Triton X-
frozen sections) - Anti-Choline Acetyltransferase
100/TBS) at 4°C for 24 hours. An Alexa
antibody (ab18736)
Fluor® 594-conjugated donkey anti-sheep
This image is courtesy of an Abreview submitted by
Carla Cabral
polyclonal (1/200) was used as the secondary
antibody.
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Immunohistochemical detection of ChAT in rat
basal forebrain using Sheep anti ChAT
(ab18736).
Rat was perfused with 1% sodium Nitrite in
0.1M TBS, pH, 7.4 for 20 min. and then fixed
with 4% formaldehyde containing 15% picric
Immunohistochemistry (PFA perfusion fixed
frozen sections) - Choline Acetyltransferase
antibody (ab18736)
acid in 0.1M TBS, pH, 7.4 for 30 min. The
brain was then cryoprotected in 30%
overnight and cut into 40 nm free-floating
sections. Sections were washed 2x with TBS
for 20 min. and passed through asending
ethanol and finally transferred to 0.1 M TBS
for rehydration for 1h.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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