Product datasheet
Anti-14-3-3 beta antibody [4E1] ab12341
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Overview
Product name
Anti-14-3-3 beta antibody [4E1]
Description
Mouse monoclonal [4E1] to 14-3-3 beta
Tested applications
WB, IP, ELISA
Species reactivity
Reacts with: Mouse, Rat
Immunogen
Recombinant full length protein (Rat).
Positive control
Mouse brain tissue extract.
General notes
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and
progression through the cell cycle by binding to and regulating several different proteins. 14-3-3
proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate
signal transduction by binding to phosphoserine-containing proteins. There are at least 7
mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype,
termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either
homo- or heterodimers which interact via their N-terminal domains and are subject to
phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasma of neurons
in the cerebral cortex and are axonally transported to the nerve terminals. They may be present
at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression
of the eta chain in cultured cell lines derived from various tumors.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.01mM Sodium Azide
Constituents: PBS, 10mg/ml Sucrose, pH 7.4
Purity
Protein A purified
Primary antibody notes
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and
progression through the cell cycle by binding to and regulating several different proteins. 14-3-3
proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate
signal transduction by binding to phosphoserine-containing proteins. There are at least 7
mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype,
termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either
homo- or heterodimers which interact via their N-terminal domains and are subject to
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phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasma of neurons
in the cerebral cortex and are axonally transported to the nerve terminals. They may be present
at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression
of the eta chain in cultured cell lines derived from various tumors.
Clonality
Monoclonal
Clone number
4E1
Isotype
IgG2b
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab12341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 29 kDa.
IP
Use a concentration of 5 - 10 µg/ml.
ELISA
Use at an assay dependent concentration. PubMed: 16367866Use at 1:5000
Dilution
Target
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized
signaling pathways. Binds to a large number of partners, usually by recognition of a
phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the
activity of the binding partner. Negative regulator of osteogenesis.
Sequence similarities
Belongs to the 14-3-3 family.
Post-translational
modifications
The alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1.
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I
to stage IV.
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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