A utumn 2008 B ringing better health to your horses TUMMY TROUBLES Gastric ulcers in performance horses: how common? CAN FOALS FIGHT BACK? Using immunity to fight R. equi infections UNIQUE LOOK Coat colour linked to eye anomalies in breeds WESTERN COLLEGE OF VETERINARY MEDICINE • EQUINE HEALTH RESEARCH FUND D o u b l e Yo u r Horse Health I N S I D E 4 The Immunity Challenge A three-year Rhodococcus equi study looks at how foals’ immune responses can be used to better protect equine babies from the bacterial infection. 6 Anna Sangster, Student Researcher Third-year veterinary student Anna Sangster talks about her summer experience as a member of a large-scale research project. 8 Tough to Stomach Dr. Fernando Marqués and his WCVM colleagues take a closer look at equine gastric ulcers in western Canadian horses. 10 Tummy Troubles A horse owner’s guide to equine gastric ulcers: the disease’s causes, clinical signs, diagnosis, treatment and long-term management. 12 The Colour Coat Connection A WCVM-based research team confirms that coat colour is linked to ocular anomalies in Rocky and Kentucky Mountain Horses. 14 Our Contributors The Equine Health Research Fund pays tribute to its generous donors during the past 12 months. FRONT COVER: Jockey Andy Scarlett balances on top of Axe Em All, a six-year-old Thoroughbred gelding owned by Joseph Quewezance and trained by Henry Keshane. The pair raced at Marquis Downs on August 30. H O R S E H E A L T H L I N E S Horse Health Lines is produced by the Western College of Veterinary Medicine’s Equine Health Research Fund. Visit www.ehrf.usask.ca for more information. Please send comments to: Dr. Hugh Townsend, Editor, Horse Health Lines WCVM, University of Saskatchewan 52 Campus Drive, Saskatoon, SK S7N 5B4 Tel: 306-966-7453 • Fax: 306-966-7274 wcvm.research@usask.ca For article reprint information, please contact sm.ridley@sasktel.net. The Western College of Veterinary Medicine begins Year 3 of the five-year matching gift incentive program for horse health! Two years after the matching gift incentive program was launched, the unique initiative has helped the WCVM raise more than $300,000 in additional funding for the college’s equine health and veterinary training programs. In August 2006, the Heather Ryan and L. David Dubé Foundation pledged to match any new donation dollars to the Equine Health Research Fund and to larger equine health research projects. The fiveyear program, which annually provides up to $100,000 in matching funding, gives WCVM the potential to raise up to $1 million for horse health programs by 2011. With Year 3 now underway, WCVM needs your help to maximize the matching gift incentive program’s annual payout. How can you help? The possibilities are endless! Just like Bob and Lois Brickley of Kennedy, Sask., demonstrate on the next page, you can donate the sale proceeds of a foal to the EHRF and double your donation to horse health. Or you can promote the Fund and the WCVM’s matching gift incentive program during your next event — something that Mary-Ann Olson of Sandcastle Farms helped to do during her farm’s 2008 Summer Sun Show Series in Saskatoon, Sask. Besides playing a vital role in improving health care for your horses, your efforts will be recognized and promoted in Horse Health Lines and in other publications. Curious to find out how you can double your horse health power? Contact Patti Tweed, WCVM’s development officer (306-966-7450; patti.tweed@usask.ca) or visit www.ehrf.usask.ca to learn how to be part of the equine health matching gift incentive program. H W hen Saskatchewan equine ranchers Bob and Lois Brickley began preparing for their 11th annual production sale this fall, the couple from Kennedy, Sask., decided to do more than sell their annual crop of registered Quarter horse foals. To kick off their part of the September Showcase Sale at Heartland Livestock in Regina, Sask., on September 13, the Brickleys donated the sale proceeds from one of their 60 foals to the Western College of Veterinary Medicine’s Equine Health Research Fund (EHRF). What made their decision even more exciting was the news that their contribution to the Fund’s equine research and veterinary training activities would double in size — thanks to a five-year matching gift incentive program that’s supported by the Heather Ryan and L. David Dubé Foundation. The longtime Saskatchewan equine ranchers, who own Moose Mountain Ranch, have donated to the veterinary college’s equine programs as part of the North American Equine Ranching She may be small and still months away from Information Council’s (NAERIC) research checkoff program for a number of years. her first birthday, but a buckskin filly named “But when I heard about the college’s new MM Mito Skip has already helped to raise matching program from our friend Jim Pollock (an EHRF advisory board member), it sounded like a great $1,050 for horse health in Western Canada. idea that we should support even further as individuals. The Brickleys, who have been part of the equine ranching industry As equine producers, we really appreciate the work that has been done at the WCVM, for 16 years, have 120 mares on their second-generation family farm and we support more horse health research in the future,” explains Bob. that’s located in the foothills of the Moose Mountains in southern The Brickleys chose to highlight Lot #1 — a five-month-old buckskin filly Saskatchewan. Besides raising purebred horses, the Brickleys own a named MM Mito Skip. “She’s an extremely quiet, very personable horse. We chose commercial herd of 250 Black Angus cattle and grow high-quality forage her because she’s a very nice filly and we knew that whoever bought her would and feed grains for their livestock. be very happy with her,” says Bob, who watched Rick and Shirley Szatkowski of In 2000, the Brickleys received the inaugural “Producer of the Year” Findlater, Sask., purchase his filly for $525. The couple had previously purchased award from the Saskatchewan Society for the Prevention of Cruelty to another Moose Mountain Ranch foal in 2006. Animals (SSPCA) in recognition of their operation’s superior animal care Through the matching gift incentive program, the sale of MM Mito Skip and excellent production environment. raised a grand total of $1,050 for WCVM’s equine health programs. Maintaining a strong equine health program at the WCVM is “We really admire Heather and David’s generosity. When people come forward integral to the entire horse industry in Western Canada, emphasizes Bob. like that, it’s just encouraging and motivating for the rest of us, and I think it’s “Equine producers across Western Canada are expecting more and more fabulous to have these types of people involved in the horse industry,” says Bob. from veterinarians. Our demands are increasing, and I think we have to MM Mito Skip’s sire is Majors Zanzabar, a six-year-old buckskin and one of support those who are providing those equine health services. We need to the Brickleys’ 12 registered Quarter horse stallions. The filly’s dam, a brown mare give them the background and research capabilities to meet our requests named MM Small Town Skips, is a descendant of one of Bob’s favourite horses: “Her and demands.” grandmother (Small Town Zoe) was a very special horse in our program, and MM As for the Brickleys’ unique way of donating to the Fund, Bob highly Small Town Skips has proven to carry those good genetics forward to her own foals.” recommends the idea to other horse breeders across Western Canada: “It’s While the Brickleys’ annual production sale mainly attracts purchasers from just a good thing to do.” across Saskatchewan and Alberta, one of their consistent buyers is Nancy Strong of Visit www.moosemountainranch.com to learn more about the Arnprior, Ont., who has purchased horses from Moose Mountain Ranch for the past Brickleys’ farm and their Quarter horse breeding program. H nine years. This year, Strong paid $3,000 — the sale’s top price — for a filly raised on the Brickleys’ farm. Above (from left to right): Lois Brickley stands in the “People buy our horses for almost anything you can think of: ranching, sales ring alongside MM Mito Skip’s new owners, performance, pleasure, breeding. Our goal is to raise high-quality, all-round Quarter Shirley and Rick Szatkowski of Findlater, Sask. horses that have a lot of colour and are easy to work with,” says Bob. A Mighty Gift for Western College of Veterinar y Medicine 3 the immunity Challenge A team of researchers at the University of Saskatchewan strives to “outwit, outplay and outlast” a deadly bacteria in the ultimate survival game for young foals. By Tess Laidlaw 4 Horse Health Lines • Autumn 2008 Ask Dr. Marianela Lopez how long she has been studying Rhodococcus bacteria and she says, laughing, “Since I was born? Well, truthfully, since I began my PhD at Washington State University in 1996 — it does seem a lifetime ago.” Lopez is a member of a new team studying Rhodococcus equi infection of foals, supported by an award from the Heather Ryan and L. David Dubé at the Western College of Veterinary Medicine (WCVM). Led by Dr. Hugh Townsend, the team is plumbing the mysteries of the newborn foal’s immune response to develop ways to protect neonates from infection. A problem in eastern Canada, the United States and internationally, R. equi is a soil bacterium that causes severe pneumonia in foals. “Currently, the key to managing this disease is early diagnosis, but this is time consuming and expensive,” says Townsend, a professor in WCVM’s Department of Large Animal Clinical Sciences. “The problem is that foals don’t tend to show signs of the disease until it has advanced to the point where it’s difficult, or sometimes impossible, to treat,” adds Lopez. “Catching foals at an early stage of the disease requires repeated examination along with ultrasound, radiographs and laboratory tests.” In all species of mammals, Lopez explains, the immune system of newborns has specific characteristics that lead to disease susceptibility. In the case of R. equi, young foals are vulnerable to disease but become immune once they’re four to five months old. Adult horses don’t become ill unless they have an immunological deficiency. “We’re interested in these characteristics because we want to see if we can change them in order to protect [foals] from disease,” says Lopez. She adds that the knowledge gained could potentially be applied to many diseases affecting the young of other species, including humans. Team R. equi Revs Up Lopez joined the U of S Vaccine and Infectious Disease Organization (VIDO) after meeting Townsend at a Rhodococcus conference in the United States. Before her arrival in 2002, research scientists at the University of Saskatchewan were not focusing on this particular problem in horses. Projects were soon underway with grants and new collaborations in Rhodococcus research. In 2004, Lopez received a two-year postdoctoral fellowship from the Saskatchewan Health Research Foundation to study the bacteria. A collaborative partnership with Dr. Mary Hondalus at the Harvard School of Public Health also allowed Townsend and Lopez to develop an R. equi model in foals, to undertake some limited work on developing a vaccine, and to begin forming a larger research team. “We needed to form an effective team of investigators to gather as much information as possible during our studies,” says Townsend. WCVM team members include Drs. Katharina Lohmann, Fernando Marqués, Steve Manning, Kristin Poirier, Navjot Kaur and Baljit Singh along with VIDO collaborators Drs. George Mutwiri and Volker Gerdts. The team’s aim is to understand the immune response of the foal as well as the genetic variations that may affect immunity in individual foals. Ideally, Townsend says the project will lead to a vaccine that will protect foals early in their lives after a single vaccination. However, it’s more likely that foals and perhaps their dams will need to be vaccinated more frequently. The Type 2 Bias Factor The crux of the problem is a characteristic of the newborns’ immune systems that generate responses with what is called a “Type 2 bias.” This bias, which is replaced by a more balanced Type 1/Type 2 immune response later in life, exists because the fetal immune response must avoid rejection of maternal cells — something that would harm the fetus. “The Type 2 bias keeps the fetus from reacting adversely to its environment — in this case, the mother’s womb – so the fetus can develop normally until it’s born,” explains Lopez. But there’s a catch: the fetus, and therefore the newborn foal’s immune system, tend not to react very vigorously to foreign cells (including bacteria) that are capable of causing infections. So, the Type 2 bias predisposes newborns to a variety of infections, such as R. equi, that a more mature immune system could battle effectively. For this reason, part of the team’s strategy will be to try and formulate vaccines that will stimulate an immune response in foals that’s more like that of an adult horse’s response. Once R. equi gain access to the lung, the bacteria actually take up residence inside one of the cell types that make up the host’s immune system. A key focus of the team’s investigation, which is under Dr. Baljit Singh’s direction, “is trying to understand why cells within the lung that are Previous page: Drs. Hugh Townsend (far left) and Marianela Lopez (centre) are two of the research scientists involved in the investigation of Rhodococcus bacteria. Top: A research team conducts a bronchoalveolar lavage on an anesthetized foal. Top right (from left to right): VIDO laboratory technician Shirley Hauta, Dr. Marianela Lopez and graduate student Navjot Kaur. Dr. Katharina Lohmann and third-year veterinary student Jill Tait tend to the foal. Bottom: Dr. Steve Manning stands by one of the foals involved in the R. equi research project. Western College of Veterinar y Medicine 5 responsible for protecting against full-fledged infection are unable to do so,” Townsend says. These cells, called macrophages, should “gobble up” the organism and digest it. But in the foal, these cells have difficulty doing this task. Once R. equi get inside these defending cells, the bacteria make the macrophages their hosts – not only surviving, but multiplying. Harnessing the Maternal Immunity Another challenge the researchers face in vaccine development is a phenomenon known as “maternal antibody interference.” Mares that have been exposed to an infectious organism produce antibodies that are then transferred to the foal in their colostrum. The antibodies, meant to protect the baby early in life, can interfere with or block the vaccine’s ability to generate an immune response in the foal. Researchers have wondered whether they could capitalize on maternal immunity by vaccinating the mare to protect the foal. “But so far, results of various studies suggest that maternal immunity stimulated in this way doesn’t fully protect foals against R. equi infection,” says Townsend. What the team hopes to achieve is to develop a vaccine strategy that will stimulate an effective immune response in the face of maternal antibodies, effectively combining the two strategies of vaccinating mare and foal. Solving this problem is a challenge that applies to many vaccines that could be given to young animals. Currently, because maternal antibodies and other characteristics of newborns’ immune systems limit immune responses early in life, most vaccines aren’t generally given to foals until they’re six to nine months old. However, because R. equi only affects foals early in life, waiting to vaccinate isn’t an effective strategy. Townsend points out another complexity: “A vaccine that would only work in foals with no maternal antibodies … wouldn’t work in environments in which this disease is common. This is because mares in these environments are naturally exposed to the organism, likely resulting in maternal antibody transfer to their foals.” Anna Sangster, student researcher Student: Anna Sangster, third-year student, WCVM. Hometown: Calgary, Alta. Background: BSc degree (zoology), University of Calgary. Summer 2008: Interprovincial Undergraduate Student Summer Research Award recipient. Mentors: Drs. Fernando Marqués and Steve Manning. Project: Fecal and nasal shedding of Rhodococcus equi in foals. Q. Can you describe your study? The 15 foals in our study were experimentally challenged with R. equi as part of the larger project (see above story). I took fecal and nasal swabs from the foals at several time points before and after the bacteria challenge. I conducted my own bacteriology work in one of the laboratories at VIDO with the help and direction of Dr. Marianela Lopez and Shirley Hauta who helped me design the study and taught me how to do the work. I plated the samples on a selective media, and then used PCR (polymerase chain reaction) and Western blot assays on colonies with appropriate morphology to try and confirm or rule out the presence of virulent R. equi. 6 Horse Health Lines • Autumn 2008 Q. What were your findings? For the most part, experimentally-challenged foals don’t shed the bacteria via fecal or nasal routes in significant numbers. Q. Do these findings support your original hypothesis? Originally, I had expected to see shedding from the foals as there’s evidence showing that naturally-infected foals do shed bacteria. The foals in this study may not have shed because of the route of infection. In our challenge model, the bacteria were placed directly into the lungs of foals, not inhaled and ingested as it would be in a natural infection. As a result, the bacteria are less likely to be colonizing in the gastrointestinal tract. Q. How did your study fit in with overall research project? I think it’s important to evaluate challenge models of disease and to see how they compare to natural infection. In the case of shedding, it appears that there are differences between the two. Shedding is important in understanding pathogenesis and epidemiology of the disease. By knowing that even foals Now midway through the project, the team has refined the R. equi disease model and continues to carry out laboratory analyses. The researchers are beginning to uncover the ways in which the immune system of the newborn differs from the adult, particularly in the response of cells in the lung. They hope to adjust these factors using specific compounds capable of modifying the immune response that can be added to a vaccine. Team members are analyzing these formulations in the lab to determine which combination will generate the most protective immune response. Going forward, the team will apply this knowledge to the development of vaccine candidates. Working with large animals such as horses means very expensive, logistically challenging experiments. In addition to the grant from the Ryan and Dubé fund, the project involves work funded by the federal Advancing Canadian Agriculture and Agri-Food Program and a pharmaceutical industry partner. Townsend continues to seek additional funding: “Although our studies are likely to inform us on many levels, this is going to be a long road.” He guesses that the R. equi problem likely has multi-faceted causes that could be environmental, genetic — or both. However, Lopez and Townsend both feel the collaborative nature of the project is what gives it its strength. “People coming from different disciplines see the problem differently, and that gives us a broader understanding,” he says. The team will also provide a foundation for future collaborations. A non-linear approach is at play on another level as well. Lopez recalls how former VIDO director Dr. Lorne Babiuk used to say that researchers investigating the workings of the immune system should try to think about the “big picture” effect of anything they learned. “You’re always trying to visualize the immune system in action versus seeing things on paper, you’re always trying to make a movie in your mind of how things work. Because there are several components to the cause of this disease, it’s critical to have a multi-disciplinary team of people playing that same movie from all different angles,” explains Lopez. “Once we understand how all of those little ‘scenes’ or pieces work together, the big picture will make sense.” H A Saskatoon freelance writer and communication instructor, Tess Laidlaw worked for six years in science communication and is pursuing a degree exploring the ways science is presented through the media. Left: Large animal resident Dr. Kristin Poirier conducts an ultrasound examination on an anesthetized foal. The team used ultrasonography (inset) to detect lesions in the foals’ lungs. Right: WCVM student Anna Sangster. with severe lung lesions may not shed the bacteria could provide some insight into how the bacteria behaves once it’s inside the body. Q. What did you learn from your research experience? I definitely learned a lot about handling mares and foals! I also learned that laboratory testing for bacteria isn’t as simple as a yes or no answer. Although we have advanced technology such as real-time PCR and Western blots, sometimes the results aren’t black and white: they still take interpretation to determine their significance. Q. What tops your list of experiences from this summer? Of course I enjoyed working with the mares and foals, but I also enjoyed the great people! There were many people involved in various aspects of this study, and although it was hard work, it was a great team effort. We had a lot of laughs, and I made some good friends! Q. Why did you decide to take part in a summer of research at WCVM? Has this experience changed your perspective of animal health research? In case I decide to pursue a research career, I wanted the challenge of designing, performing and analyzing results of a project from start to finish. I certainly learned that animal health research is more difficult than I thought, and I now greatly appreciate the amount of work that goes into understanding diseases and producing safe, effective vaccines and treatments. Q. Would you recommend a research experience? I definitely recommend applying for the research program. Even if you don’t think you are going into research as a career, I think the summer experience gives you a much better understanding of the whole process and improves your critical thinking of other research — a skill that’s important for anyone in the scientific field. Regardless of the project’s results, you’re sure to learn a lot through the process itself and you will certainly develop good relationships with your supervisors and fellow researchers. Western College of Veterinar y Medicine 7 Equine Gastric Ulcers Tough to Stomach As awareness grows, more people are taking a closer look at equine gastric ulcers and their potential impact on horses’ performance and quality of life. Out of sight, out of mind. For Dr. Fernando Marqués, that old saying aptly describes the issue of equine gastric ulcers in today’s horse industry. Studies conducted in other areas of North America and in other parts of the world show that equine gastric ulcer syndrome (EGUS) affects horses of all ages and all breeds. In particular, there’s a high prevalence of EGUS among horses competing in racing, dressage, show jumping, endurance and western performance events. For example, some veterinary researchers estimate that between 80 and 90 per cent of racehorses in training are affected by gastric ulcers. However, no research team has determined yet the prevalence of gastric ulcers among western Canadian horses. Without that important information, Marqués says it’s still challenging to convince people of the potential seriousness of equine gastric ulcers and their impact on horses’ performance. “If a horse had a painful lesion on its leg, there would be no question about providing the animal with some kind of treatment. But because we can’t see the ulcers and nobody knows how much these lesions actually affect the horse, there are still questions about whether we should treat them or not,” points out Marqués, a specialist in large animal internal medicine at the Western College of Veterinary Medicine (WCVM). People’s perspectives usually change when they can see a gastric ulcer with their own eyes. That can be done with the use of a gastroscope, a piece of equipment that looks like an ordinary endoscope — only much longer. By passing the device’s long tube into the horse’s stomach, veterinarians can use the endoscopic camera to gain a clear view of the inner reaches of a horse’s stomach and identify any lesions. 8 Horse Health Lines • Autumn 2008 “To me, it’s similar to the issue of whether horses feel pain: different people have different perspectives on how horses show pain. The same applies for gastric ulcers, because at this point, nobody knows how much a gastric ulcer affects a horse. But if it’s there, and we can prove it’s there, I don’t think we should ignore it and say they’ll be fine. I think we need to take a closer look at how these lesions are affecting them.” Right tool for the job But for Marqués and his colleagues at WCVM’s Veterinary Teaching Hospital, diagnosing gastric ulcers in equine patients has been challenging with the hospital’s existing equipment. The limitation? A short gastroscope. “Our older gastroscope is classed as a three-metre gastroscope, but it’s actually only 2.1 metres in length. It works well for ponies and small horses, but for an average-sized, 450-kilogram horse, we can’t do a full examination because we can barely see into the animal’s stomach,” explains Marqués. “We could be missing potential problems in the far end of the stomach and duodenum.” That diagnostic challenge disappeared this summer when the veterinary college gained ownership of a new gastroscope that’s a full three metres in length and includes a portable digital recording and imaging system. “This gastroscope gives us the complete picture: we no longer have to be concerned about missing lesions in the far end of a horse’s stomach,” says Marqués. While the gastroscope is a huge diagnostic asset for WCVM’s large animal clinicians and their equine patients, the new equipment is valuable for several other key reasons at the veterinary college. Besides its use as a teaching tool for WCVM’s undergraduate veterinary students, interns and residents, Marqués will be taking the gastroscope on the road during the Above: Thoroughbred racehorses come down the stretch at Saskatoon’s Marquis Downs. Studies show that 80 to 90 per cent of racehorses in training are affected by gastric ulcers. next few months for a series of eight continuing education sessions with western Canadian veterinarians and their clients. “The main goal is to create more awareness of equine gastric ulcers and the potential impact they have on horses,” says Marqués, who conducted his first session in mid-September at the South West Animal Health Centre in Swift Current, Sask. “During each session, I give a presentation about gastric ulcers for local Above: Dr. Fernando Marqués conducts a gastroscopic examiveterinarians and their clients. Then I demonstrate how to use the nation on an equine patient during a continuing education gastroscope on some of the clinic’s own patients — horses that session at the South West Animal Health Centre for local veteriare suspected of having gastric ulcers.” narians in the Swift Current, Sask., area. Photos courtesy of Dr. Fernando Marqués, WCVM. In co-operation with Marqués, Merial Canada Inc. is organizing and sponsoring the continuing education sessions throughout Western Canada. The international animal health company also contributed $15,000 toward the final purchase of WCVM’s new that the team’s ultimate goal is to examine 100 horses by the end of next gastroscope. year’s racing season. “We’re also interested to see if there’s any link between “These sessions will help to give veterinarians and horse owners more horses with gastric ulcers and other issues such as poor performance, insight into a disease that’s probably just as prevalent in this region as it is lameness issues and other concurring diseases.” in other areas,” says Marqués. After veterinarians have a better idea about the prevalence of EGUS in western Canadian horses, Marqués says other studies can be done to look at Prevalence Pursuit potential diagnostic options, treatment and management of the disease. And that’s where the new gastroscope will fulfil another key purpose: “For example, how you feed a horse can contribute to the development research. With the technology now in place, Marqués says he and his of gastric ulcers. Studies that look at how different feeding regimes and the colleagues have the equipment they need to pursue the question of gastric size of feed that people provide for their horses affect the prevalence of EGUS ulcers’ prevalence in western Canadian horse populations. in a group of horses are potential projects that would help us learn more As a first step, a WCVM-based research team conducted gastroscopic about managing this disease.” examinations on 40 normal, healthy racehorses that were in active Eventually, Marqués hopes that all of these activities — continuing training and competition at Saskatoon’s Marquis Downs this summer. The education, teaching, clinical services and research — will encourage more examinations took place late in the 2008 racing season. The horses’ owners western Canadian veterinarians and horse owners to consider equine gastric also filled out questionnaires about their horses’ age, sex, performance and ulcers as a legitimate health issue. medical history, and WCVM researchers will now analyze these details in And as awareness grows in people’s minds, so will the need for more correlation with the gastroscopy findings. specific research to help answer some of the unknowns about this “out of “This ongoing study will show us how many animals in this sight” disease. H population of racehorses have gastric ulcers,” explains Marqués, adding Western College of Veterinar y Medicine 9 Visit any show ring, riding arena or racetrack in North America and the odds are good that many of the equine athletes that you see have equine gastric ulcer syndrome (EGUS). But this condition isn’t limited to performance horses: EGUS also affects young foals and many pleasure horses. EGUS describes a variety of clinical symptoms including erosions and ulcers in the lower portion of the esophagus, the glandular and nonglandular portions of the stomach, and the proximal duodenum (the beginning of the small intestine). Gastric ulceration affects a large number of horses of all ages: it’s been shown to be prevalent in 25 to 50 per cent of foals up to two months old, while another study reported that between 80 and 90 per cent of racehorses in training have gastric ulceration. The following article is a condensed version of “Equine Gastric Ulcer Syndrome” by Dr. Fernando Marqués, a specialist of internal medicine at the Western College of Veterinary Medicine’s Department of Large Animal Clinical Sciences. The original article appeared in the March 2007 issue of Large Animal Veterinary Rounds — a large animal veterinary publication that’s produced at WCVM. Visit www.larounds. ca to read the original article and to access other equinerelated health management information. 10 Horse Health Lines • Autumn 2008 TUMMY TROUBLES Equine gastric ulcer syndrome (EGUS) The equine stomach Horses are natural grazers that are designed to continuously eat around the clock. In relation to their size and overall feed consumption, horses have relatively small stomachs that consist of two distinct regions. The proximal portion of the stomach is the non-glandular, squamous cell-lined region while the distal portion of the stomach is the fundic glandular portion. A stepped edge called the margo plicatus divides the non-glandular and glandular regions. Ulcers form in horses’ stomachs when there’s an imbalance between the factors that incite erosion and the factors that protect the stomach. The most common inciting factor is hydrochloric acid while bile acids and pepsin may also play a contributing role in disease development. Risk factors for the development of gastric erosion and ulceration include intermittent feeding, increased exercise intensity, and dietary factors such as feeding high-concentrate, low roughage diets to horses. For young horses, it’s possible that illness — and the stress associated with being sick — cause ulcer development since the prevalence of gastric ulcers in critically ill foals is higher. Clinical signs and diagnosis While a horse’s history and a description of clinical signs are important in diagnosing EGUS, veterinarians rely on an endoscopic examination to make a definitive diagnosis. Most practitioners use a grading system that was developed by the Equine Gastric Ulcer Council • Proton pump inhibitors (such as omeprazole) have been to classify the gastric lesions: the system ranges from Grade 0 (normal) to effective in healing NSAID-induced gastric ulcers as well as naturallyGrade 4 (severe ulceration). occurring cases of EGUS. Omeprazole was effective in reducing or • Adult horses: clinical signs of EGUS can include low-grade colic, eliminating the severity of gastric ulcers in Thoroughbred racehorses poor body condition and decreased performance. But signs can vary: some undergoing intensive training. However, omeprazole is expensive and adult horses with endoscopic evidence of gastric ulcers may show no signs veterinarians have questioned the efficacy of compounded preparations. or very subtle symptoms while other horses may show more typical clinical • Sucralfate is effective in treating peptic ulcers in humans, but signs. its efficacy in treating ulcers in the equine gastric squamous mucosa is In adult horses, veterinarians most often find lesions in the gastric squamous mucosa — especially along the margo plicatus. Lesions in the pyloric region (opening from the stomach into the small intestine) are also important. • Neonatal foals with gastric ulceration may suffer from colic and diarrhea, grind their teeth or salivate continuously, have little or no appetite, and tend to lie on their backs. Since very few foals with endoscopic evidence of EGUS show symptoms, chances are the ulceration is severe if you observe any of these clinical signs. The gastric squamous mucosa is where veterinarians find most gastric lesions in young foals. Physiologic stress associated with illness has also been linked with gastric ulcers in neonatal foals: those lesions are found in the glandular epithelium. • Older foals: clinical signs like diarrhea, poor appetite, poor growth and poor body conditions are associated with severe squamous epithelial lesions. Foals with duodenal ulceration often present similar clinical signs as the ones associated with gastric ulceration such as colic, teeth grinding, continuous salivation and diarrhea. They may also Risk factors for the development of gastric erosion suffer from delayed emptying of their stomachs and gastroesophageal reflux. and ulceration include intermittent feeding, increased exercise intensity, and dietary factors such as feeding Treatment Since excess acid exposure is high-concentrate, low roughage diets to horses. the main reason behind squamous mucosal erosion and ulceration, most veterinarians turn to anti-ulcer therapies with the aim of suppressing or neutralizing gastric acid. unknown. Sucralfate may be effective for treating stress-induced ulcers in • H2 antagonists can successfully raise the gastric pH and resolve neonatal foals, but so far, there’s no clinical evidence to support that theory. gastric ulcers in foals and adult horses. But the degree and duration of • Antacids can reduce gastric acidity in horses, but their effects acid suppression by H2 antagonists varies from horse to horse. Practitioners are short-lived (last for approximately two hours) and require large doses routinely administer anti-ulcer drugs to critically ill neonatal foals as a several times a day. prophylactic measure, but its effectiveness remains controversial. Treatment • Prokinetics can be part of a therapy plan when veterinarians responses vary and there’s also a concern that the use of prophylactic antisuspect delayed gastric emptying without any physical obstruction in a ulcer therapy may suppress the function of gastric acidity in preventing patient. This treatment is also useful in treating foals with duodenal disease bacterial translocation in neonatal foals. and gastroesophageal reflux. H Western College of Veterinar y Medicine 11 The Coat Colour Connection H orses come in a fantastic array of colours from black to white, from bays to greys, and with or without varying amounts of white. The glorious, spotted coats of Appaloosas are a common sight for western Canadians and so are golden palominos — thanks to Roy Rogers and Trigger. On the other hand, silver dapple is a colour that’s still on the unusual side. The colour occurs in several breeds — including the gaited Rocky Mountain Horse — in which the typical, smoky chocolate By Roberta Pattison coat with flaxen mane and tail is often seen and highly admired. Veterinarians aren’t usually concerned with the coat colour of their equine patients, but when a particular colour or pattern is associated with disease conditions or Findings from a new, abnormalities that can affect horse health, it becomes an issue. Such is the case with congenital stationary night blindness (CSNB) in Appaloosas. A few years WCVM-based study ago, a research team led by veterinary ophthalmologists at the Western College of Veterinary Medicine confirm that coat colour (WCVM) confirmed that CSNB is linked with horses that are homozygous for the leopard spotting is linked to the ocular gene (see the Summer 2008 issue of Horse Health Lines for the latest update, www.ehrf.usask.ca). Members of that same team, headed by veterinary ophthalmologist Dr. Bruce Grahn, have anomalies that affect one also been investigating eye anomalies in purebred and crossbred Rocky Mountain and Kentucky of out every two Rocky Mountain Horses. The study’s findings, which were published in the July 2008 issue of the Canadian Veterinary and Kentucky Mountain Journal, support the long-standing theory that these anomalies appear to be associated with Horses in Canada and colour — specifically, silver dapple. However, the WCVM study’s results also question two previous about the mode of inheritance and the precise nature of these anomalies. the United States. The conclusions Besides Grahn, the research team included Dr. Chantale Pinard of the Faculté de médecine College’s researchers also vétérinaire at the Université de Montréal and WCVM veterinary ophthalmologist Dr. Lynne Sandmeyer. genetic aspects of the study were conducted by Dr. George Forsyth of WCVM’s Department of question two long-standing The Veterinary Biomedical Sciences, Dr. Rebecca Bellone of the University of Tampa, and Sheila Archer, conclusions about the a Saskatchewan-based phenotype researcher. Heather Ryan and L. David Dubé Veterinary Health Research Fund, which was created in mode of inheritance and 2006 toThesupport multi-year equine health research projects at the WCVM, provided funding for the the precise nature of the two-year research study. eye abnormalities found in these two breeds. 12 Horse Health Lines • Autumn 2008 Does colour indicate a problem? Unlike the eyes of Appaloosas suffering from CSNB, which appear completely normal on ophthalmic examination, the eyes of Rocky Mountain Horses show a variety of obvious lesions. While veterinary ophthalmologists have identified defects of the iris, cornea, retina and eyelids, fluid-filled cysts of the ciliary body (a muscular ring located in the front part of the eye) is the most common condition. In most cases, affected horses don’t suffer from significant visual impairment but rupture of these cysts occasionally leads to retinal detachment and affected vision. While this condition is congenital (present at birth) and inherited, it isn’t progressive. The association between the eye anomalies in Rocky Mountain Horses and the silver dapple colour is undeniable. But the exact nature of the association — whether or not the same gene is responsible for both the eye abnormalities and the horse breed’s colour — has yet to be determined. Tracking the expression of the dilution gene that produces silver dapple is somewhat difficult since the gene only affects eumelanin (black pigment) and not pheomelanin (red pigment). In other words, chestnut horses (whose coats contain no black pigment) may carry the silver dapple mutation, yet they will look no different from chestnut horses that do not carry the gene. Meanwhile, a silver bay whose black points have been diluted but has a red body coat may look very much like a flaxen-maned chestnut horse at first glance. These kinds of situations require close study and analysis, points out Grahn. “We have two coat colour experts on the team, but coat colour genetics is still an imprecise science.” Question of inheritance The WCVM-based research study includes horses that come from two herds — living on opposite sides of Canada — that are linebred within their own ranks but unrelated to each other. One herd includes 97 purebred and crossbred Rocky Mountain Horses, while the second herd consists of 37 Kentucky Mountain Horses. The incidence of ocular anomalies within this population of horses is close to 50 per cent, consistent with the findings of earlier research done elsewhere involving these two breeds. During the project, veterinary ophthalmologists examined the eyes of purebred or crossbred Rocky and Kentucky Mountain Horses as well as the eyes of horses that were unrelated in breeding. The specialists used a transilluminator, a biomicroscope and an indirect ophthalmoscope to examine the horses’ eyes after their pupils were dilated. Next, the team constructed a pedigree with related horses to investigate the mode of inheritance of the multiple eye anomalies and their relationship to coat colour. Coat colour experts assessed all of the horses, and photographs of all animals involved in the study were archived. After a detailed pedigree analysis, the research team was able to confirm that the mode of inheritance of the ocular anomalies in Rocky Mountain and Kentucky Mountain Horses is an incomplete penetrance of a dominant inherited trait. “Our conclusion is based on the fact that when mares with no relation to Rocky or Kentucky Mountain Horses were bred to affected Rocky Mountain stallions, there was a range of outcomes. Some offspring had complete, multiple ocular anomalies, some had temporal ciliary cysts, while other foals were completely normal,” explains Grahn. “These findings are inconsistent with the codominant mode of inheritance.” For many years, veterinarians and horse owners have described the collection of eye anomalies associated with silver dapple colour as anterior segment dysgenesis (ASD), because of the apparent similarities to anterior segment lesions that are well-documented in other species — including humans. Grahn says it’s certainly possible that Rocky Mountain horse eye anomalies develop partially as ASD. However, after close examination of the affected horses involved in this study, he and his colleagues found none of the lens-related diseases or abnormalities that are usually associated with ASD. The WCVM research team also observed that the corneas in affected horses weren’t significantly different in shape from the corneas in non-affected horses. This observation concurs with what other researchers have previously found in earlier studies. Work still needs to be done to determine whether the gene controlling silver dapple colour, which has now been identified, is also responsible for the ocular anomalies. While many researchers believe this will turn out to be the case, Grahn is skeptical. “We don’t think it’s the same gene, but another gene close by on the same chromosome. We have a black horse with the condition that isn’t considered silver dapple, although some people think this classification must be a mistake. I don’t agree. We also have a silver dapple horse that’s clear [of ocular abnormalies] and there may be others. We need to extend the pedigree.” With most of the ophthalmic research completed, Grahn says the investigation of ocular anomalies in Rocky and Kentucky Mountain Horses is now in the hands of the geneticists on the research team: “The area where the gene resides is known, and we’re getting close.” H Roberta Pattison is a freelance writer who is a regular contributor to the national publication, Dogs in Canada. Recently retired from grain farming, she still lives on her farm near Delisle, Saskatchewan. Previous page: Large temporal ciliary cysts — fluid-filled cysts of the ciliary body — are the most common lesions in Rocky and Kentucky Mountain horses. Above (left): This image of a Rocky Mountain Horse’s eye shows retinal degeneration secondary to previous cysts. Above (right): This third image shows focal areas of retinal degeneration related to previous cysts. Photos: Dr. Bruce Grahn, WCVM. Western College of Veterinar y Medicine 13 Our Contributors A list of contributors to the Equine Health Research Fund during the period between September 1, 2007, and August 31, 2008. Visit www.ehrf.usask.ca for donor information. $25,000 and up $100 to $499 Heather Ryan and L. David Dubé Foundation Inc., Saskatoon, SK American Association of Equine Practitioners (AAEP), Saskatoon, SK • Appaloosa Horse Club of Canada, Claresholm, AB • Burns, Beverley, Edmonton, AB • Campbell, Kathleen, Calgary, AB • Canadian Pony Club, Manitoba Region, Baldur, MB • Colchester & District Agricultural Society, Sherwood Park, AB • Corbett, Bill, Calgary, AB • Critchley, Prue, Hamiota, MB • Crouch, Katy, Saskatoon, SK • Crush, Ken, Langham, SK • Delaney Veterinary Services Ltd., Sherwood Park, AB • Dorit De Bruyn, Eva, Winfield, BC • Dowler, Leslie, Edmonton, AB • Elders Equine Clinic, Cartier, MB • Fitzharris, Fern, Saskatoon, SK • Fulton, Tammie, Calgary, AB • Greeno, Tanya, Maple Ridge, BC • Gregory, Marilyn, Langley, BC • Horse Industry Association of Alberta, Airdrie, AB • Laing, Robert, Leader, SK • Lloydminster Animal Hospital, Lloydminster, AB • McKague, Ross and Brenda, Brandon, MB • Misra, Vikram, Saskatoon, SK • Okotoks Animal Clinic, Okotoks, AB • Palese, Kathleen, Calgary, AB • Palouse Holdings Ltd., Calgary, AB • Perron, Michael, Surrey, BC • Regina District Dressage Association Inc., Regina, SK • Roth, Melanie, Indian Head, SK • Saskatchewan Regional Pony Club, Saskatoon, SK • Saskatchewan Pleasure Driving The Heather Ryan and L. David Association, Dubé Foundation will match Saskatoon, SK any new contributions to the • Schaefer, Equine Health Research Fund. Maria, New Learn how you can double your Dundee, ON horse health donation dollars by $10,000 to $24,999 Anonymous • Horse Racing Alberta, Edmonton, AB • Saskatchewan Liquor and Gaming Authority, Regina, SK. $1,000 to $9,999 Alliance Energy Ltd., Regina, SK • British Columbia Standardbred Breeders’ Society, Surrey, BC • Chouinard, Lynn, De Winton, AB • Cymbaluk, Nadia, Carberry, MB • Du Mont, Mark and Patricia, Aldergrove, BC • Leach, Rex and Beverley, Swan River, MB • MacEwen Almon, Saskatoon, SK • Martin, Peter, Edmonton, AB • Moore & Co. Veterinary Services Ltd., Balzac, AB • North American Equine Ranching Information Council (NAERIC), Louisville, KY • Townsend, Hugh, Saskatoon, SK • United Way of Calgary — Donor Choice Program, Calgary, AB. $500 to $999 Killeen, James, Sherwood Park, AB • Paton & Martin Veterinary Services Ltd., Aldergrove, BC • Riddell, Betty (in memory of Murray Riddell), Saskatoon, SK • Sask Valley Riding Club, Warman, SK • Saskatchewan Horse Federation Inc., Regina, SK • Southern, Ron and Margaret, Calgary, AB • Thomson, Thelma, Woodstock, ON • Urtasun, Frances, Edmonton, AB. Photos: A late summer sunset gilds Thoroughbred racehorses at Marquis Downs in Saskatoon, Sask. 14 Horse Health Lines • Autumn 2008 DID YOU KNOW? visiting www.ehrf.usask.ca. A Year in Review • Schellhorn, Garcia, Saskatoon, SK • Scheppner, Melissa, Surrey, BC • SED Systems Social Committee, Saskatoon, SK • Souris Valley Trekkers, Estevan, SK • Story, Lionel, Netherhill, SK • Taylor, Samuel and Nancey, Swift Current, SK • Thul, Lori, Moose Jaw, SK • Townsend, Robert, Victoria, BC • West Wind Veterinary Hospital Ltd., Sherwood Park, AB • Wild Rose Arabian Horse Association, Stony Plain, AB • Wilson, George, Saskatoon, SK • Wolf, Lori, Lumsden, SK. Up to $99 Baller, F. Marion, Regina, SK • Boughen, Teresa, Calgary, AB • Burford, Karen, Calgary, AB • Burlingame, Donna, Saskatoon, SK • Cadman, Dorothy, Airdrie, AB • Callfas, Debbie, Lloydminster, SK • Coulthard, Colleen, Castor, AB • Davies, Deanne, Chilliwack, BC • Dobson, Yvonne, Kenosee Lake, SK • Duncan, Robert, Cut Knife, SK • Eagle Butte Ranches Ltd., Calgary, AB • Elaschuk, Norm, Turin, AB • Ellis, Betty, Cayley, AB • Feist, Dawn, Asquith, SK • Halina, Karen, Saskatoon, SK • Hazelton, Janene, Burns Lake, BC • Komm, Kenneth, Sherwood Park, AB • Lenz, Bernice, Calgary, AB • Lester, Vance, Saskatoon, SK • Lower Island Equestrian Club, Saanichton, BC • Quesnel & District Riding Club, Quesnel, BC • Robinson, Brian, Lloydminster, AB • Rothwell, Janet, West Vancouver, BC • Saskatchewan Peruvian Horse Club Inc., Saskatoon, SK • Seidlitz, Robert, Comox, BC • Silver Spurs 4-H Club, Christopher Lake, SK • Smith, Jeffrey and Hannelore, Saskatoon, SK • Theilman, Laura, Saskatoon, SK • Thompson, Donald, Dalmeny, SK • Thomson, Helen, London, ON • Townsend, Robert, Saskatoon, SK • Twidale, John, Surrey, BC • Unger, Diane, Langley, BC • Walker, Diane, Okotoks, AB • Wallace, Betty, Dauphin, MB • Zeilner, Catherine, Furdale, SK. The Equine Health Research Fund’s statement of revenue, expenditures and fund balances for the year ended, December 31, 2007. EXPENDABLE fund 2007 Revenue Donations Private* $180,702.48 Horsemen’s Association 40,000.00 Racing Commissions 33,970.00 NAERIC** 14,000.00 Miscellaneous - 268,672.48 Expenditures Fellowship program $81,204.81 Grants 107,225.00 Recovery from previous grants - Summer student 8,100.00 Graduate student awards ($3,000 each) 6,000.00 Fundraising 27,443.32 Horse Health Lines 37,886.67 Administration - Advisory Board 4,142.96 Equipment - 272,002.76 Deficiency of revenue over expenses (3,330.28) Transfer from restricted funds 3,330.28 Unrestricted funds, beginning of year - 2006 $25,800.00 20,000.00 21,840.00 - 67,640.00 $63,881.14 59,141.55 (6,180.43) 7,200.00 6,000.00 19,570.27 49,216.29 3,993.42 - 202,822.24 (135,182.24) 135,182.24 - Fund balance, end of year 0.00 0.00 **************************************************************** RESTRICTED FUND 2007 2006 Investment income $19,551.15 $169,241.21 Transfer to unrestricted fund (3,330.28) (135,182.24) Fund balance, beginning of year 1,795,003.47 1,778,782.60 Fund balance, end of year $1,795,003.47 $1,778,782.60 *Private donations (2007) include the 2007 and 2008 donations of $25,000 per year from the Heather Ryan and L. David Dubé Foundation. **The North American Equine Ranching Information Council (NAERIC) donated $7,000 per year in 2006 and 2007. The 2007 total includes both donations. Photo: Oktober Revolution Photograpy GALLOPING GAZETTE RUN FOR HORSE HEALTH: Delineator’s Dream, a 10-year-old dark bay gelding, was the winner of the Equine Health Research Fund Feature on Saturday, Aug. 30, at Saskatoon’s Marquis Downs racetrack (above). Owned by Rivers North and trained by Sam Neubuhr, the horse finished the six-furlong race in 1:14.3 with Marquis Downs’ top jockey Tim Moccasin astride. As part of the race feature, WCVM’s Equine Health Research Fund awarded an embroidered cooler to the winning racehorse. The Fund presented the cooler in recognition of the Saskatchewan racing industry’s longtime support of equine health and veterinary training programs at the veterinary college. WCVM representatives who came out to cheer on the horses included Drs. Steve Manning, Sheryl Gow, Sue Ashburner, Nathalie Tokateloff, Kristin Poirier and Nora Chavarria. Second-year veterinary students Dayne Tatariuk and Danyse Lewis were also on hand to host the EHRF booth along with WCVM development officer Patti Tweed. Earlier in the evening, a 10-horse field competed in the $25,000 Prairie Lily Stakes — one of Marquis Downs’ main stakes races of its racing season. The winner was J.C.’s Action, ridden by Moccasin and owned by Jack Harasyn. The annual race is open to two-year-old horses that participated in the previous year’s Prairie Lily Yearling Sale. As part of the day’s event, the Fund’s booth was also on display at the 2008 Prairie Lily Sale that was held at Prairieland Centre. Nearly 30 two-yearolds participated at this year’s sale that’s organized by the Saskatchewan division of the Canadian Thoroughbred Horse Society. THE COACH OF CHAMPIONS: Firstyear WCVM veterinary student Erin Borrow from Maple Ridge, B.C., was the proud coach of two award-winning Canadian Pony Club (CPC) teams in the Prince Philip Games’ National Masters and National ‘A’ Division Championships this summer. The teams, whose members are part of the East Maple Ridge Pony Club, trained with Borrow from February to August in preparation for the national team competitions. The first triumph came in late August when the five female members of Borrow’s Masters team won the CPC’s National Finals after competing against top teams from Alberta and Ontario. The CPC Masters division consists of riders ranging in age from 15 to 21 years old. “My team had to go to Edmonton, Alta., for their Nationals without me as the competition was held on Wednesday, August 27 — the third day of classes for me at WCVM,” explains Borrow. “However, I did practice with them up until the week before their competition, and evidently, the practice paid off!” Ten days later, Borrow flew to Elora, Ont., to help the five members of her ‘A’ team compete at the National ‘A’ Division Championships. The three boys and two girls ranged in age from 10 to 14 years. On September 6, the East Maple Ridge team competed against the top teams from Ontario, Alberta and Nova Scotia. “After 20 races, they ended up in a tie breaker for second place, and they just lost by the skin of their teeth to come third,” says Borrow. “They were much younger than the other competitors and this was their first National Final so I was really proud of them.” She adds that when Champions (cont’d) the teams ride out of province, team members ride on pooled ponies (supplied mounts) — another challenge in the national competition. The Prince Philip Games, named after His Royal Highness Prince Philip, consist of team gymkhanas in which four out of five team members participate in each race. “The races involve a lot of technical skill,” explains Borrow, adding that almost all of the skills are done at a gallop. “Some races see riders picking tennis balls off cones, shooting balls through a goal with a hockey stick, or putting mugs on poles. Some races also involve the riders getting off and vaulting back on — also at a gallop!” With a full year of veterinary courses ahead of her, Borrow has appointed an assistant to coach her national teams until she completes exams next spring: “We’re looking forward to doing even better next year.” ALBERTA HORSE CONFERENCE: Looking for high-quality information about horse health, management, nutrition and training? Mark January 9 to 11 on your 2009 calendar. Those are the dates for the Horse Industry Association of Alberta’s 27th annual Horse Breeders and Owners Conference at the Capri Centre in Red Deer, Alta. This year’s line up of topics includes equine dentistry with Dr. Roxy Bell (WCVM ’75), new concepts in parasite control with Dr. Ray Kaplan of the University of Georgia and a presentation on developing strength and flexibility with the University of Michigan’s Dr. Hilary Clayton, an internationally-known expert in equine locomotion and a former WCVM faculty member. A team representing WCVM’s Equine Health Research Fund will be part of the conference’s trade show. Make sure to stop in at the EHRF booth and find out what’s new in horse health at the College! For more conference details, visit www.albertahorseindustry.ca. Visit Horse Health Lines online at www.ehrf.usask.ca PUBLICATIONS MAIL AGREEMENT NO. 40112792 RETURN UNDELIVERABLE CANADIAN ADDRESSES TO: Research Office, WCVM University of Saskatchewan 52 Campus Drive Saskatoon, SK S7N 5B4 wcvm.research@usask.ca Printing Services Document Solutions • 306-966-6639 • University of Saskatchewan • CUPE 1975