Product datasheet Anti-COX2 / Cyclooxygenase 2 antibody ab6665 10 References Overview Product name Anti-COX2 / Cyclooxygenase 2 antibody Description Rabbit polyclonal to COX2 / Cyclooxygenase 2 Specificity The antibody does not cross react with Cox-1. Tested applications WB, ELISA, Dot Blot Species reactivity Reacts with: Mouse, Rat, Human Immunogen Recombinant fusion protein (Human C-terminal end) Epitope carboxy-terminus of human Cox-2. General notes COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle. Storage buffer Preservative: 0.01% Sodium azide Purity Whole antiserum Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab6665 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes WB ELISA Dot Blot 1 Application notes Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. This antiserum against Human Cox-2 and has been tested for use in immunoblotting (1:5,000 to 1:10,000) and ELISA (1:10,000 to 1:50,000). The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Target Function Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Pathway Lipid metabolism; prostaglandin biosynthesis. Sequence similarities Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain. Post-translational modifications S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. Cellular localization Microsome membrane. Endoplasmic reticulum membrane. Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2