Report to the Oregon Processed Vegetable Commission 2007–2008 1. Title: Broccoli Breeding, Evaluation and Seed Production 2. Project Leaders: James R. Myers, Horticulture Brian Yorgey, Food Science and Technology 3. Cooperators: None 4. Project Status: Terminating 30 June, 2008 5. Project Funding: $6,795 breeding $3,159 processing $9,954 total Research efforts were focused on testing various inbred combinations for hybrid production, and evaluating commercial cultivars for adaptation to Oregon growing conditions and for head rot resistance. Breeding funds were used for a major portion of the support of a vegetable breeding technician, student labor, supplies, and research farm expenses. Processing funds were used for processing samples of experimental hybrids. 6. Objectives: I. Develop broccoli varieties adapted to western Oregon with suitable quality, high yields, and disease resistance. Specific traits include: a. Early to midseason maturity b. Concentrated yield potential c. Downy mildew & head rot resistance d. Exerted heads with large openly branched heads with heavy, clean stem for easy trimming and separation into spears and chunks e. Firm, uniform florets of good color f. Fine beads with short pedicels, which are retained after freezing II. 7. Evaluate cytoplasmic male sterility (CMS) as a method for producing F1 hybrid seed. Report of Progress: Greenhouse inbred and hybrid seed production: Cuttings were taken from inbreds and breeding lines grown in the field in 2006 to establish material for crossing and hybrid seed production in the greenhouse during the winter of 2006-2007. A total of 14 cross combinations (Tables 1 & 6) were obtained. Yield Trial: Eight hybrid combinations were tested in replicated yield trials (Tables 1 & 2, Figure 1). Entries were seeded in the greenhouse on 11 June, and then transplanted on 19 July into 30 ft rows spaced 30 in between rows with four replications. Within row spacing was 12 in. between plants. Data were collected at harvest maturity on plant and 2007-2008 Broccoli Breeding Progress Report head characteristics, and yield, and a sample of each hybrid was sent to the OSU Food Science and Technology Pilot Plant for processing and evaluation. Data from the Pilot Plant had not been completed at the time of this report and will be reported separately. Three check hybrids (Regal, Arcadia, and Emerald Pride) were included for comparison to experimental hybrids. Five of the experimental hybrids had maturities similar to Emerald Pride and Arcadia (52-53 days) while two were earlier and one was slightly later. Two experimental hybrids (S387 x S446, and S449 x S454) had yields (net ton/acre) statistically equal to Regal, the hybrid with the highest yield (Table 1, Figure 1). Only one hybrid (S448 x S454) had significantly lower net yield compared to Emerald Pride and Arcadia. The trial was much more uniform this year than last, with much lower numbers of immature (young) and over mature (cull) heads. We continue to observe significantly lower proportion of leaves (4-11% of total weight) attached to the stem of harvested heads for OSU experimental hybrids compared to commercial checks (22-30%) (Table 1). This year we initiated a pilot study to examine proportion of the head that constitutes florets relative to stem. This arose from the observation that while many OSU hybrids have lighter heads than the checks, they have proportionally more of their weight concentrated in the florets. We floretted a sample of ten heads per line and calculated florets as a percent of total head weight. The data were not replicated, but it appears that most OSU hybrids and Arcadia and Emerald Pride have similar percent florets, whereas Regal had a lower ratio of florets to total head weight. Next year we will repeat this effort with replication to provide a statistical basis for our observations. From observation data on the yield trial (Table 2), we identified 2 new hybrids (S448 x S454 and S449 x S454) that show potential, although S448 x S454 had lower yield, and S449 x S454 had a fairly flat head and lacked good segmentation but had excellent yield. Four previously evaluated hybrids that did well were S387 x S446, S387 x S454, S445 x S454 and S447 x S454, with S387 x S446 having very good yield, S387 x S454 and S445 x S454 showing excellent uniformity, and S447 x S454 looking especially good for several qualities including head shape, stem color, exsertion, segmentation and uniformity. When examining hybrids that have been tested over several years (table 3), we found that S387 x S454 had good yields again this year, unlike last year, when it had very low yields compared to previous years. Last year’s data were apparently due to uneven plant stands and uniformity caused by our transplanting method. S445 x S454 appears to have consistently good performance across years. A head rot screening trial of commercial broccoli hybrids and selected OSU lines was planted on 20 July in 30 in. rows, and thinned to a 9 in. within row spacing. Data on head rot incidence and severity were recorded at actual maturity for each variety. Head rot incidence and severity in ‘Emerald Pride’ (susceptible check) was 88% and 6.3 on a 9 point scale, respectively (Table 4). Several commercial hybrids had significantly less head rot than the most susceptible hybrid (Arcadia), including Heritage, Avenger, Iron Man, Everest, Green Goliath, Marathon, and Maximo. Hallmark, which showed very good resistance last year, had very poor stand this year and was not included in the data. It is not clear why Arcadia had such a poor performance with head rot because this hybrid is usually considered to be more resistant than Emerald Pride, but we may have been late in collecting data for this hybrid. Of the OSU material, S446 x S454 showed very poor 2007-2008 Broccoli Breeding Progress Report resistance (not statistically different from Arcadia, the most susceptible variety in the trial), while S387 x S410, S447 x S454, and S387 x S454 showed susceptibility in the range of Emerald Pride. The OSU OP population showed some resistance again this year, as did (CMS 13-1 x S454) x S446 (both statistically equal to Maximo, the best in the trial). It is perhaps not surprising that the OSU OP population shows some resistance, since it has been selected for head quality and performance under Oregon growing conditions for the past seven years now. It is interesting that the CMS and normal versions of S454 when crossed to S446 yield such different results. It may be possible that the CMS source brought in additional traits for head rot resistance that are not present in the OSU materials. Empirical observations this year revealed that the overriding factor in head rot resistance in broccoli appears to be bead size and density. This would explain why Hallmark with its very fine (intermediate to cauliflower) bead size had such high levels of resistance in 2006. Heads in the button to 5 cm (2 in) stage were generally not susceptible to head rot, and then became most susceptible from 5 cm up to about 9 cm (3.5 in), with head rot susceptibility decreasing (but never to the same low levels as young heads) as heads mature. Observation Trials: These trials included 22 inbreds, 11 lines under selection, and 13 hybrid combinations (Table 5 and 6). After evaluation, cuttings from inbreds were rooted in the greenhouse to be used in selfing and crossing during the winter of 2008. Among lines under selection, 04-3-2-2 stands out as being exceptional for large heads with very find beads on a highly exserted stem (Table 6). This line will be fast-tracked for hybrid combining ability testing. We determined that these plots were uniform enough to continue with propagation and selection on a plot, rather than an individual plant, basis. Six of the hybrids in the observation trial were tests of CMS lines as parents, which generally performed well (table 6). Of the normal (non-CMS) hybrids in the observation trial, S449 x S387 looked especially good, with very nice heads and good uniformity. S387 x S448 also looked very good. A number of inbred line cross combinations have been tested in the past 8 years (Table 7) but additional testing is still needed among the S440 series inbreds. Cytoplasmic Male Sterile (CMS) Facilitated Hybrid Seed Production: Selected OSU inbreds (S442, S445 and S446) were used as pollen parents in isolated crossing blocks at the Vegetable Farm. They were planted in combination with a set of CMS lines which had been backcrossed into one of three inbred backgrounds (S410, S411, and S454). The trials were used to evaluate potential crossing ability with 3 OSU inbreds, and to produce seed (table 8). Not all combinations worked well, but some did, with one cross, (CMS 14-1 x S411) x S445 being especially successful. 8. Summary: Trials at the Vegetable Research Farm included a yield trial of OSU derived hybrids, observation trials of OSU inbreds and hybrids, and head rot trial of commercial hybrids and some OSU hybrids. We see progress in the OSU material with the most recently derived inbreds being the best. It is of interest that the OSU OP population had a relatively low head rot incidence, and perhaps we are making progress in selecting material more tolerant of head rot. Another season of evaluation would be useful in this regard. 2007-2008 Broccoli Breeding Progress Report z Table 1. Yield data from a hybrid broccoli trial, Corvallis, 2007. Gross T/A Young Heads T/A Culls T/A Net T/A Net Heads/A Head Diameter (cm) % Leaves % Floy rets 0.9 3.64 0.02 2.6 4.60 0.05 0.00 3.40 15827 11.7 6.1 55 0.07 4.27 15246 12.4 4.6 60 29.0 5.1 4.40 0.07 0.00 4.17 15101 13.5 3.7 57 49 27.8 3.5 3.75 0.07 0.04 3.31 14665 11.6 9.0 57 S446 x S454 52 28.8 3.5 4.78 0.14 0.03 4.07 14810 12.7 11.3 55 S447 x S454 52 28.8 5.2 4.63 0.12 0.09 4.16 12923 13.6 6.3 54 S448 x S454 48 28.5 8.9 3.42 0.08 0.22 2.79 12342 11.7 9.8 54 S449 x S454 52 28.5 1.8 4.60 0.03 0.01 4.30 15827 13.1 5.7 58 Arcadia 53 28.5 0.9 6.40 0.18 0.25 4.09 13504 12.0 29.7 57 Emerald Pride 52 28.3 4.5 5.52 0.07 0.00 4.23 14956 12.8 22.4 59 Regal 46 28.0 4.6 6.96 0.08 0.01 5.21 14810 12.5 23.9 52 1.2 5.7 0.78 0.11 0.10 0.95 2183 0.9 5.9 Days to Harvest No. Plts/ Plot % Blind Plants S387 x S443 52 28.5 S387 x S446 55 29.0 S387 x S454 53 S445 x S454 Hybrid LSD 0.05 z Planted July 19 in 30' plots, rows 30" apart, thinned to 12" between plants. Mean of four replications. y % florets calculated as head weight minus stem weight for 10 heads; not replicated. 2007-2008 Broccoli Breeding Progress Report Table 2. Observation notes from a broccoli yield trial, Corvallis, 2007. Line S387 x S443 S387 x S446 S387 x S454 Maturity z (days) Head Ht (cm) Plant Ht (cm) Head y Shape Bead x Size Stem w color Exserw tion Segmentw ation Uniforw mity Branchw ing Overall 52 55 53 60 53 51 65 58 56 6 6 4 C M M 7 7 7 8 6 7 5 5 7 6 6 7 1 3 4 5 7 7 w S445 x S454 49 50 58 4 F-C 7 6 4 8 1 8 S446 x S454 52 48 54 6 M-C 7 6 3 5 3 5 S447 x S454 S448 x S454 52 48 44 52 51 62 7 6 F F 8 7 7 7 8 7 8 5 3 5 9 8 S449 x S454 Arcadia Emerald Pride Regal 52 53 52 46 36 47 46 37 44 64 67 48 4 5 5 6 M F M M 7 6 6 5 5 4 3 3 3 2 1 1 6 3 5 5 3 3 3 7 6 3 4 4 z Planted July 19. Scale of 1 - 9 where 1 = concave and 9 = extreme dome. x F = fine; M = medium; C = coarse. w Scale of 1-9 where 1 = poor and 9 = excellent. y 2007-2008 Broccoli Breeding Progress Report Notes Basal lodging Strong apical dominance; 1 bolter Basal lodging; variable maturity Basal lodging; variable maturity Strong apical dominance Variable maturity z Table 3. Yield data (net T/A) from eight years of broccoli yield trials, Corvallis, 2007 Entry S387 x S410 S387 x S411 S387 x S438 S387 x S443 S387 x S445 S387 x S446 S387 x S454 S396 x S445 S396 x S446 S396 x S454 S398 x S438 S398 x S445 S398 x S446 S398 x S454 S399 x S453 S399 x S454 S400 x S438 S400 x S445 S400 x S446 S400 x S453 S400 x S454 S410 x S442 S410 x S445 S410 x S446 S410 x S447 S410 x S453 S410 x S454 S411 x S445 S411 x S446 S411 x S453 S411 x S454 S438 x S442 S440 x S446 S442 x S411 S442 x S454 S443 x S454 S445 x S454 S446 x S454 S447 x S410 S447 x S411 S447 x S454 S448 x S454 S449 x S454 Arcadia Excelsior Legend Regal Emerald Pride LSD 0.05 2000 2001 2002 Tons/Acre 2003 2004 4.62 2.45 2005 1.39 2006 2007 2.82 3.4 3.79 3.83 2.73 4.14 1.83 2.64 1.88 5.67 3.66 4.12 5.40 6.40 3.34 1.32 4.27 4.17 4.90 4.30 4.24 2.56 4.37 5.20 4.99 3.26 3.36 1.84 3.06 3.90 3.05 2.87 2.36 5.10 3.80 2.32 3.87 3.43 3.44 3.79 3.72 5.35 3.58 3.70 2.96 3.49 3.96 4.22 5.70 1.36 1.46 3.79 5.30 3.30 4.23 2.72 4.22 3.92 5.28 3.65 5.10 3.65 3.14 4.07 3.73 2.43 3.23 3.95 3.63 0.52 0.84 3.78 4.32 4.97 4.71 1.39 6.10 3.70 3.40 4.83 3.68 4.29 3.41 2.31 2.24 3.90 3.43 2.25 4.45 5.30 3.40 0.90 4.17 4.54 0.93 3.30 4.32 0.79 3.62 3.91 z Uneven number of plots in 2006 prevented calculation of LSD. 2007-2008 Broccoli Breeding Progress Report 3.31 4.07 4.16 2.79 4.30 4.09 5.21 4.23 0.95 z Table 4. Head rot incidence and severity in selected broccoli cultivars and lines from a head rot trial, Corvallis, 2007 Entry Arcadia Avenger Emerald Pride Everest Green Goliath Green Magic Heritage Iron Man Liberty Marathon Maximo Packman Premium Crop Tradition Source Maturity Date Sakata 12-Nov Sakata Sakata Johnny's Seminis Sakata Seminis Seminis Seminis Sakata Sakata Nichols Takii Seminis 18-Nov 10-Oct 22-Oct 16-Nov 1-Nov 18-Nov 21-Nov 12-Nov 16-Nov 21-Nov 6-Oct 22-Oct 14-Nov Total No. Plants Rep Rep Rep 1 2 3 30 30 26 27 21 27 26 27 26 30 28 26 25 28 25 27 29 24 25 28 29 26 24 23 28 25 24 24 24 25 24 26 27 28 23 28 27 27 23 31 25 25 y % Infected Rep Rep Rep 1 2 3 100 100 92 59 86 33 88 100 69 90 93 62 52 86 96 56 59 88 64 29 86 58 63 87 46 56 96 79 92 76 92 31 63 93 70 39 100 56 22 52 88 100 Average % Infecty ed Severity 97 5.7 65 88 43 60 93 66 64 93 55 43 78 88 82 4.0 6.3 2.3 3.7 7.7 3.0 2.7 6.3 4.3 3.7 4.3 6.3 3.3 x OSU OP Population (CMS 13-1 x S454) x S446 S387 x S410 S387 x S454 OSU OSU OSU OSU 10-Oct 14-Nov 30-Oct 6-Oct 27 21 25 19 25 24 23 16 24 29 25 18 52 86 100 68 76 58 100 63 46 52 56 67 58 65 85 66 4.3 3.3 5.3 7.0 S446 x S454 S447 x S454 LSD 0.05 OSU OSU 30-Oct 12-Nov 25 26 28 26 25 23 100 77 100 73 84 70 95 73 25 7.0 5.0 2.5 z Planted July 20 in 30" rows, thinned to 9" between plants. Percent of infected heads at maturity for each variety, based on actual counts. x Average of 3 reps; scores based on a 1-9 scale with 9 = severe y 2007-2008 Broccoli Breeding Progress Report Notes Leaves cover heads Variable; severity scores range from 2-9 Even immature heads are infected z Table 5. Broccoli observation trial, OSU inbreds, Corvallis, 2007. Maturity (days) Head Ht (cm) Leaf Canopy Ht (cm) Head Diam (cm) Head y Shape Stem x Color Bead w Size Head Exserx tion Head Segmentx ation Plot Unix formity Branch x -ing Overall x Score ORCR1 HS143 HS179-1 S233B 82 47 44 53 38 31 53 43 49 46 48 50 8 7 10 14 5 7 6 8 5 3 5 7 F C C C 3 3 9 6 1 1 1 1 3 3 7 9 5 7 7 3 3 3 5 5 S315 82 51 65 16 7 3 F 6 8 7 5 7 S352 60 43 68 11 4 5 C 3 1 5 1 3 S370 46 49 42 13 7 7 M-C 9 1 7 5 6 S387 66 50 64 11 7 5 C 7 7 1 7 5 S403 S410 S411 S437 S438 S440 53 53 60 53 53 60 47 43 55 42 44 50 50 52 57 54 55 45 13 12 13 16 10 13 7 7 5 6 6 7 5 5 7 7 7 5 M F F C F C 8 6 7 7 7 9 7 8 7 2 5 3 3 6 3 6 6 3 1 3 5 3 5 5 6 7 7 5 6 6 S442 82 49 59 12 6 5 F 6 9 5 3 4 S443 S445 53 60 53 58 59 55 13 12 7 6 7 5 F-C M 7 9 5 3 1 6 5 3 5 7 Inbred 2007-2008 Broccoli Breeding Progress Report Notes Chlorotic lower leaves Uneven stand Strong apical dominance Uneven stand; strong apical dominance Some leafy heads Poor stand; variable maturity Uneven maturity; some aphid infested heads Uneven maturity Small heads Uneven maturity Some heads huge but loose and full of tiny leaves Variable bead size; uneven maturity z Table 5. Broccoli observation trial, OSU inbreds, Corvallis, 2007 (cont). Maturity (days) Head Ht (cm) Leaf Canopy Ht (cm) Head Diam (cm) Head y Shape Stem x Color Bead w Size Head Exserx tion Head Segmentx ation Plot Unix form-ity Branch x -ing Overall x Score S446 60 51 65 13 6 5 F 6 3 1 1 6 S447 S448 66 53 62 59 58 61 15 10 6 8 5 5 F F 8 8 7 8 3 7 1 5 6 7 S449 S454 60 60 38 55 50 57 17 12 6 7 7 7 F-M F 3 8 3 7 5 6 1 7 4 0 Inbred z Planted July 19 in 30" rows, thinned to 12" apart. Scale of 1-9 where 1 = concave, 3 = flat, 5 = slight dome, 7 = moderate dome and 9 = extreme dome. x Scale of 1-9 where 1 = poor and 9 = excellent. w F = fine, M = medium, C = coarse. y 2007-2008 Broccoli Breeding Progress Report Notes Variable maturity Variable maturity; some leafy heads Soft heads Nice conventional type z Table 6. Broccoli observation trial, OSU hybrids and breeding lines, Corvallis, 2007. Maturity (days) Head Ht (cm) Leaf Canopy Ht (cm) Head Diam (cm) RS1-1-2-3 53 72 69 04-3-2-2 53 57 04-3-2-3-1 47 04-3-2-4-2 Bead w Size Head Exserx tion Head Segmentx ation Plot Uniformx ity Branchx ing Overall x Score 3 F 9 9 3 7 7 6 7 F 8 8 8 3 9 Tallest in field; head loose & soft Huge uniform headslarge heads become soft 13 5 7 F-M 6 5 3 3 7 Uneven maturity 66 13 7 7 F 7 9 5 7 6 Leaves in head 52 43 18 6 3 F 7 5 7 9 6 53 45 55 13 8 7 M 7 3 9 1 8 Uneven bead size Lacks good segmentation but otherwise very nice 04-4-2-2 53 44 55 14 5 7 F 6 7 6 1 7 04-5-2-1-3 53 53 55 13 8 4 M-C 7 1 5 7 5 Small heads 04-5-2-2-2 53 50 67 15 4 7 F 6 5 5 1 7 04-5-2-2-3 53 54 67 14 5 8 F 7 4 3 3 7 Heads lack purple cast Still segregating but overall nice type 04-5-3-3-2 53 47 60 15 7 8 M 6 7 7 5 7 S387 x S445 47 45 57 13 5 5 C 6 1 5 1 5 S445 x S387 47 45 56 13 6 7 M 6 3 5 1 6 S387 x S447 60 58 64 13 5 9 F 6 5 3 5 4 S387 x S448 53 66 71 11 8 5 F 9 5 8 3 8 S387 x S449 53 54 64 15 5 7 M 7 4 8 3 8 S449 x S387 60 61 61 17 4 7 F 6 5 8 5 9 S443 x S454 44 47 52 15 3 7 C 7 2 5 3 5 Head y Shape Stem Colx or 15 8 63 19 48 52 60 59 04-4-1-1-3 60 04-4-1-1-4 Hybrid 2007-2008 Broccoli Breeding Progress Report Notes Variable bead size Small heads but very tall & exserted Very nice heads z Table 6. Broccoli observation trial, OSU hybrids and breeding lines, Corvallis, 2007 (cont). Maturity (days) Head Ht (cm) Leaf Canopy Ht (cm) Head Diam (cm) CMS1-1 x S454 BC2 60 53 52 CMS6-1 x S411 BC4 53 45 CMS7-2 x S410 BC4 60 CMS12-2A x S410 BC4 Bead w Size Head Exserx tion Head Segmentx ation Plot Uniformx ity Branchx ing Overall x Score 5 F 7 8 6 3 7 9 3 M 7 5 3 3 5 12 5 7 M 6 6 4 3 5 55 15 6 7 F 7 0 5 3 7 56 63 13 7 7 F 6 8 4 5 7 Variable plot 57 72 13 5 5 M 7 3 3 3 6 Variable maturity Head y Shape Stem Colx or 14 7 59 17 52 58 60 52 CMS13-1 x S454 BC2 60 CMS14-1B x S411 BC4 53 Hybrid z Planted July 19 in 30" rows, thinned to 12" apart. Scale of 1-9 where 1 = concave, 3 = flat, 5 = slight dome, 7 = moderate dome and 9 = extreme dome. x Scale of 1-9 where 1 = poor and 9 = excellent. w F = fine, M = medium, C = coarse. y 2007-2008 Broccoli Breeding Progress Report Notes z Table 7. Inbred line combinations tested in 8 years of broccoli yield trials, Corvallis, 2007 . Inbred Line S410 S411 S438 S442 S443 S445 S446 S447 S453 S454 S387 S396 S398 S399 S400 S410 S411 S438 S440 S442 S443 S445 S446 S447 S448 S449 2 0 0 0 0 1 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0 0 0 0 1 1 1 0 1 1 1 0 0 0 0 3 2 2 0 1 2 3 0 1 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 7 3 2 1 4 5 5 0 0 3 1 5 4 3 1 1 z Number in each cell indicates the number of times this combination has been tested. 2007-2008 Broccoli Breeding Progress Report z Table 8. Seed Set and Harvest for OSU Broccoli CMS Inbred Lines, Corvallis, Oregon, 2007 Crossed To OSU Inbred % Silique Set Weight of Harvested Seed (g) CMS 5-3 x S410 S442 near zero 0 CMS 7-2 x S410 S442 near zero 0 CMS 6-1 x S411 S442 1 1.3 CMS 14-1 x S411 S442 near zero 0 CMS 9-1 x S454 S442 1 1.1 CMS 13-1 x S454 S442 25 7.3 CMS 5-3 x S410 S445 10 5.1 CMS 7-2 x S410 S445 near zero 0 Inbred CMS 6-1 x S411 S445 variable 7.6 CMS 14-1 x S411 S445 50 70.0 CMS 9-1 x S454 S445 near zero 0 CMS 13-1 x S454 S445 near zero 0 CMS 5-3 x S410 S446 none 0 CMS 7-2 x S410 S446 none 0 CMS 6-1 x S411 S446 1 1.7 CMS 14-1 x S411 S446 near zero 0 CMS 9-1 x S454 S446 1 0.9 CMS 13-1 x S454 S446 25 6.2 Notes Variable seed set from plant to plant Most plants with less than 0.01% silique set; one plant with much better set--this was saved as a single plant Variable seed set from plant to plant Planted May 1 in 3 separate, isolated crossing blocks, based on OSU inbred parent. 2007-2008 Broccoli Breeding Progress Report Figure 1. Broccoli Yield Trial 2007 5.50 5.21 4.17 4.23 4.27 4.30 S387 x S446 S449 x S454 Arcadia 4.16 Emerald Pride 4.09 S387 x S454 4.07 S446 x S454 4.50 S447 x S454 5.00 4.00 3.50 T/A 3.00 3.31 3.40 2.79 2.50 2.00 1.50 1.00 0.50 Line 2007-2008 Broccoli Breeding Progress Report Regal S387 x S443 S445 x S454 S448 x S454 0.00