SRIHARI RADHAKRISHNAN
126 Beedle Drive, Apt 209 Ames, IA 50014
(515) · 509 · 8565 srihari@iastate.edu
www.public.iastate.edu/˜srihari
SUMMARY
r Doctoral candidate in Bioinformatics with research focus on the transcriptional, epigenetic and chromosomal underpinnings of Genotypic (GSD) & Temperature-dependent Sex Determination (TSD) systems in
turtles
r Primarily interested in next-generation sequencing (NGS) analysis with 3+ years of experience on nextgeneration sequencing analysis of genomes, epigenomes and transcriptomes with published work in journals
and conference posters
r Possess working knowledge of statistical methods and toolkits used in next-gen sequencing pipelines
r Proficient in R, Perl & Shell scripting with 3+ years of experience in high performance computing clusters
EDUCATION
Ph.D., Bioinformatics & Computational Biology
Aug 2010 Iowa State University
GPA: 3.84/4.0
Major Advisor: Dr. Nicole Valenzuela Co-Major Advisor: Dr. Peng Liu
Coursework: Fundamentals of Genome Informatics, Advanced Genome Informatics, Empirical methods for Computer Science, Computational Functional Genomics & Systems Biology, Structural Genome Informatics, Statistical
Design and Analysis of RNA-seq and Microarray experiments, Applications of next-generation sequencing data
processing software in Genomics
B.E. (Hons.) Computer Science
Birla Institute of Technology & Science, Pilani, India
Aug 2004 - Jun 2008
GPA: 8.38/10.0
DISSERTATION RESEARCH
Aim: Identification of candidate genes implicated in TSD pathways using transcriptomics & methylomics; in silico
identification of sex chromosome sequences from whole genomes
Experience: Transcriptome assembly using genome guided & de novo methods; transcript quantification and
count normalization, differential expression and pathway analyses, gene enrichment analysis; Methylome assembly
and differential methylation analysis, de novo genome assembly and abundance estimation
PUBLICATIONS
H.B Shaffer,..., Srihari Radhakrishnan & 13 others, The Western Painted Turtle genome: the evolution of extreme physiological adaptations in a slowly evolving lineage, Genome Biology. doi:10.1186/gb-2013-14-3-r28
Amy L Toth, John F Tooker, Srihari Radhakrishnan, Robert Minard, Michael T Henshaw and Christina M
Grozinger. Shared genes related to aggression, rather than chemical communication, are associated with reproductive dominance in paper wasps (Polistes metricus), BMC genomics. 15.1 (2014): 75.
INDUSTRY EXPERIENCE
Maverix Biomics Inc.
June - Aug 2013
Bioinformatics Intern
San Mateo, CA
· Compared genome-guided RNA-seq pipelines for precision testing gene expression
· Built automated pipeline to assemble de novo, quantify and annotate large RNA-seq datasets.
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Histogenetics India Pvt. Ltd
June 2008 - May 2010
Team Lead, Bioinformatics
Chennai, India
Built submission pipelines for novel HLA-alleles to GenBank, WHO & BMDW
Developed fast and efficient algorithms to compare HLA alleles & genotypes against large-scale databanks
Led a team of three engineers to design support services for HLA tissue typing client requests
Developed backend support services for DATRI (www.datriworld.org), a bone marrow donor registry based in
Chennai, India
CONSULTING EXPERIENCE
BCBLab @ Iowa State University
Oct 2012 - May 2013
Project Title: Transcriptomal analysis of anterior segment in cats with and without glaucoma
Client: Dr. Norman M. Ellinwood
TECHNICAL STRENGTHS
Programming Languages
Technologies
OS Platforms
R, Perl/BioPerl, Shell scripting, C, C#, JavaScript
ASP.NET, MSSQL, MATLAB
Unix/Linux, Windows, Mac OS
GRANTS & AWARDS
Teaching Excellence Award
ISU-HHMI Teaching Fellowship
Grants-in-aid of Research
Merit-cum-Need Scholarship
Iowa State University
Howard Hughes Medical Institute
The Sigma-Xi Research Society
BITS, Pilani, India
Dec
Jan - May
Dec
Aug 2004 - Jun
2013
2013
2012
2008
PRESENTATIONS
Srihari Radhakrishnan, Andrew J. Severin, Robert Literman and Nicole Valenzuela, Comparative transcriptomic dynamics during embryonic gonadal development in turtles with genotypic and temperature-dependent sex
determination presented at the Plant and Animal Genomics conference XXI, San Diego
Jan 2013
Delegate for Histogenetics India Pvt. Ltd. at the 33rd Annual Scientific Meeting of the Australasian and South
East Asian Tissue Typing Association (ASEATTA), New Delhi
Nov 2009
TEACHING EXPERIENCE
Graduate Teaching Assistant
Iowa State University
· Currently teaching an exploratory biology lab using a hands-on approach to biodiversity
· Over 350 undergraduate students taught in 3 years+
Aug 2011 Ames, IA