Application Note
USD 3068
Mixing in a Partially Filled Pall Biocontainer with
the PadMixer® System
Mixing system: PadMixer 50 system
Mixing biocontainer: 25 L Mixer biocontainer
Application mixing type: Solid-liquid
The PadMixer system is a scalable single-use mixing system. The heart of this mixer
is an innovative top-mounted mixing paddle that allows effective and uniform mixing
in demanding applications, such as preparation of contained high-solids solutions
or mixing of high-viscosity liquids.
Introduction
Mixing biocontainers for PadMixer systems are available in
capacities of 25 L, 50 L, 200 L, 500 L and 1000 L. However,
it is often necessary for users to mix in biocontainers that
are only partially filled. This experiment demonstrates how
solid-liquid mixing can be performed effectively in as little
as 16% of the biocontainer capacity.
In this experiment, a PadMixer 50 system was used to
prepare various volumes of salt solution. After a baseline
experiment at full biocontainer capacity, a series of mixing
experiments were performed using progressively smaller
volumes of liquid in the same size biocontainer until
incomplete mixing was encountered.
Experimental
DI water and sodium chloride were mixed in a full-to-capacity 25 L biocontainer, making
a 25 g/L NaCl solution. Once uniformly mixed, the conductivity of the solutions was
measured. This conductivity (40 mS) was taken to represent the conductivity of a fully
and uniformly mixed solution of this concentration.
A fresh 25 L biocontainer was then filled with 5 L of DI water and 125 g of sodium
chloride, and mixed under identical mixing conditions. This was repeated at 4 L/100 g
and at 3 L/75 g. In each case, the conductivity of the solution was measured at the end
of the mixing cycle.
Results
As can be seen on Figure 1, mixing was complete and uniform down to 4 L, or only 16% of the biocontainer capacity.
Figure 1
Mixing efficiency in 25 L biocontainer
50
45
Conductivity / mS
40
35
30
25
20
15
10
5
0
25
20
15
10
Liquid V
olume / L
Volume
o
5
0
Conclusions
The paddle mixing action of the PadMixer system is capable of effective solid-liquid mixing, even in volumes as low as
16% of biocontainer capacity.
Visit us on the Web at www.pall.com/mixing
E-mail us at allegro@pall.com
Corporate Headquarters
Port Washington, NY, USA
+1.800.717.7255 toll free (USA)
+1.516.484.5400 phone
biopharm@pall.com e-mail
International Offices
Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria,
Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico,
the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden,
Switzerland, Taiwan, Thailand, the United Kingdom, the United States, and Venezuela. Distributors in all major
industrial areas of the world. To locate the Pall office or distributor nearest you, visit www.pall.com/contact.
European Headquarters
Fribourg, Switzerland
+41 (0)26 350 53 00 phone
LifeSciences.EU@pall.com e-mail
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
Asia-Pacific Headquarters
Singapore
+65 6389 6500 phone
sgcustomerservice@pall.com e-mail
© 2015, Pall Corporation. Pall,
and PadMixer are trademarks of Pall Corporation. ® indicates a trademark
registered in the USA and TM indicates a common law trademark. Filtration.Separation.Solution. is a service
mark of Pall Corporation.
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