Application Note
USD 3062
Heating in a 25 L Cubical Mixing Biocontainer
with the PadMixer® System
Mixing system: PadMixer 1000 system
Mixing biocontainer: 25 L Mixer biocontainer
Application mixing type: Liquid-liquid
The PadMixer system is a scalable single-use mixing system. The heart of this mixer
is an innovative top-mounted mixing paddle that allows effective and uniform mixing
in demanding applications, such as preparation of contained high-solids solutions
or mixing of high-viscosity liquids.
Introduction
The cubical design of the single-use mixing biocontainer
delivers superior mixing efficiency because of the way its
shape disrupts laminar flow and promotes thorough mixing.
This mixing efficiency also ensures uniformity of temperature
throughout the mixing biocontainer.
In this experiment, the ability to increase temperature to
a set-point in the mixing biocontainer of a PadMixer 1000
system, and to hold at that set-point, was evaluated.
Experimental
A 25 L mixer biocontainer was installed in a double-jacketed 25 L vessel, and filled with 25 L
of water that had been pre-chilled to 7 °C. The vessel was connected to the PadMixer 1000
system, and then to a Lauda◆ T4600 heater/chiller unit.
A set-point temperature of 80 °C was programmed into the heater/chiller unit, and mixing
was initiated at a speed setting of 100 rpm and a mixing angle of 10°. A Pt-100 sensor
located in the center of the biocontainer was used to monitor the temperature.
Results
Figure 1 shows the temperature in the center of the mixing biocontainer as a function of time.
The 25 L of water was heated from 7 °C to 80 °C over a period of 1½ hours. During the linear heating period, heating
rates as high as 73 °C/hour were achieved.
Figure 1
Temperature readings
100
Temperature / °C
80
60
Targeted temperature inside
25 L biocontainer
Temperature of heating liquid
inside double jacket
Temperature of water inside
25 L biocontainer
40
20
0
0
20
40
60
80
100
Time / Minutes
120
140
160
Conclusions
The paddle mixing action of the PadMixer 1000 system facilitates rapid temperature adjustments inside
a cubical biocontainer.
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E-mail us at allegro@pall.com
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Port Washington, NY, USA
+1.800.717.7255 toll free (USA)
+1.516.484.5400 phone
biopharm@pall.com e-mail
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industrial areas of the world. To locate the Pall office or distributor nearest you, visit www.pall.com/contact.
European Headquarters
Fribourg, Switzerland
+41 (0)26 350 53 00 phone
LifeSciences.EU@pall.com e-mail
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
Asia-Pacific Headquarters
Singapore
+65 6389 6500 phone
sgcustomerservice@pall.com e-mail
© 2015, Pall Corporation. Pall,
and PadMixer are trademarks of Pall Corporation. ® indicates a trademark
registered in the USA and TM indicates a common law trademark. Filtration.Separation.Solution. is a service
mark of Pall Corporation. ◆Lauda is a trademark of Lauda Dr. R. Wobser GMBH & Co. Kg
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