Application Note
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Demonstrating Temperature Uniformity in Cubical
Mixing Biocontainer with the PadMixer® System
Mixing system: PadMixer 1000 system
Mixing biocontainer: 200 L Mixer biocontainer
Application mixing type: Liquid-liquid
The PadMixer system is a scalable single-use mixing system. The heart of this mixer
is an innovative top-mounted mixing paddle that allows effective and uniform mixing
in demanding applications, such as preparation of contained high-solids solutions
or mixing of high-viscosity liquids.
Introduction
The cubical design of the single-use mixing biocontainer
delivers superior mixing efficiency because of the way its
shape disrupts laminar flow and promotes thorough mixing.
This mixing efficiency also ensures uniformity of temperature
throughout the mixing biocontainer.
In this experiment, the uniformity and stability of temperature
throughout the mixing biocontainer of a PadMixer 1000
system was evaluated.
Experimental
A 200 L biocontainer was installed in a double-jacketed 200 L vessel, filled with 150 L of water, and presented to the
PadMixer 1000 system. The vessel was then connected to a Lauda◆ T4600 heater/chiller unit, and the temperature
was set to 40 °C. Mixing was initiated at a speed of 30 rpm and a mixing angle of 10°.
To measure temperature uniformity, Pt-100 temperature sensors were placed in 13 different positions inside the mixing
biocontainer (8 corners, 4 sides and the center of the biocontainer; locations A-M in Figure 1). Once the set-point temperature
had been reached, temperature measurements were made throughout the biocontainer.
Figure 1
Temperature uniformity
Results
Table 1 shows the statistics for temperature readings from the 13 sensors.
Table 1
Sensor temperature readings
Minimum temperature
Maximum temperature
Mean temperature
Standard deviation
39.4 ºC
39.6 ºC
39.5 ºC
0.07 ºC
Conclusions
The paddle mixing action of the PadMixer 1000 system facilitates a high degree of temperature uniformity inside a cubical
biocontainer, even when operated at its least vigorous, lowest-shear settings.
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The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
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© 2015, Pall Corporation. Pall,
and PadMixer are trademarks of Pall Corporation. ® indicates a trademark
registered in the USA and TM indicates a common law trademark. Filtration.Separation.Solution. is a service
mark of Pall Corporation. ◆Lauda is a trademark of Lauda Dr. R. Wobser GMBH & Co. Kg
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