Application Note
USD 3073
Mixing Uniformity in Cubical Mixing Biocontainers
with the PadMixer® System
Mixing system: PadMixer 50 system
Mixing biocontainer: 50 L Mixer biocontainer
Application mixing type: Solid-liquid
The PadMixer system is a scalable single-use mixing system. The heart of this mixer
is an innovative top-mounted mixing paddle that allows effective and uniform mixing
in demanding applications, such as preparation of contained high-solids solutions
or mixing of high-viscosity liquids.
Introduction
The cubical design of the single-use mixing biocontainer delivers superior mixing efficiency
because of the way its shape disrupts laminar flow and promotes thorough mixing.
In this experiment, a PadMixer 50 system was fitted with a 50 L mixer biocontainer, and used
to prepare 50 L of a 25 g/L sodium chloride solution. Although the mixing speed range for this
mixer is 30-150 rpm, a speed at the low end of this range was chosen to demonstrate mixing
efficiency under the least favorable conditions.
Experimental
A 50 L cubical biocontainer was filled with
45 L of room temperature DI water and
1125 g of sodium chloride. Once mixed,
this would result in a solution containing
approximately 2.5% w/v NaCl. The mixing
biocontainer was then connected to the a
PadMixer 50 system, and mixing performed
at a setting of 50 rpm and a
mixing angle of 20°.
Figure 1
Position of the Conductivity Sensors
To measure mixing uniformity, conductivity
sensors were placed in 12 different positions
inside the mixing biocontainer (see A-L in
Figure 1). Conductivity measurements were
recorded every 5 second. This experiment was performed in triplicate.
Results
Figure 2 shows the mean conductivity readings over time for each of the 12 sensors.
Note the elevated conductivity indicated by sensor “A” during the first 40 seconds of mixing. This sensor was located in
the corner where the solid sodium chloride was delivered at the beginning of each experiment, and demonstrates the
speed with which the solid material was dispersed within the biocontainer.
It can be seen from this chart that mixing was completed within approximately 400 seconds, and that uniformity within the
biocontainer was excellent.
Conductivity / mS
Figure 2
Average Mixing speed 50 L biocontainer
50.00
45.00
40.00
35.00
30.00
25.00
20.00
15.00
10.00
5.00
0.00
A
B
C
D
E
F
G
H
I
J
K
L
0
100
200
300
400
500
Time / Seconds
600
700
Conclusions
The paddle mixing action of the PadMixer 50 system is capable of high mixing efficiency and uniformity inside a cubical
biocontainer, even when operated at its least vigorous, lowest-shear settings.
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The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
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© 2015, Pall Corporation. Pall,
and PadMixer are trademarks of Pall Corporation. ® indicates a trademark
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