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The Xpansion multiplate bioreactor is part of the Pall Life Sciences’ single-use bioreactor family, designed for shear-sensitive adherent cell applications, such as cultivation of stem cells.
The Xpansion multiplate bioreactor was developed for safe, large-scale production of traditional 2-D cell cultures. The multiplate structure is comprised of a large cell growth surface area (up to 122,400 cm 2 ), while the compact design enables the elimination of the gas phase between the plates. This gas phase is replaced by an automatically controlled aeration system which provides advanced gas diffusion. Control is automatic via disposable pH and dissolved oxygen (DO) sensors. Temperature monitoring and agitation control are also included. Xpansion bioreactors offer the capability of monitoring cell morphology and density through the use of an optional digital holographic microscope.
The Xpansion system includes a single-use bioreactor, docking station and a controller.
Single-Use Bioreactor with Stacked Multiplate Design
Comprised of stacked hydrophilized polystyrene radial plates, the Xpansion bioreactor is designed to promote cell growth on the top of each plate; each plate has a hole in the center. The stacking of these plates forms the central column which allows circulation of the medium in the bioreactor and gas exchange.
The plates are covered by an outer tube, a head plate on the top and a base plate on the bottom. On the head plate, there are:
Six sensor connectors: pH, DO, and four optional sensors
Three viewing windows for use with optional holographic microscope
Two vent filters
Two gas filters (in and out) for aeration system
One thermowell port
One sampling port
In the bottom side of the bioreactor, there are:
Two fluid operation ports (e.g., inoculation, harvest, etc.)
One magnetic impeller
13
2
1
3
10
9
2
1
4
5
6
7
8
13
11
12
The Xpansion Multiplate Bioreactor
1
2
Gas lines
(in & out)
Vent.1
C-Flex ◆ tubing and silicone 3/16 in. ID, 0.2 μm hydrophobic filters (PTFE, polyester, polypropylene)
C-Flex 3/16 in. ID, 2 0.2 μm hydrophobic filters (PTFE, polyester, polypropylene)
3 Sampling port C-Flex 3/16 in. ID with needleless connector for sampling with luer syringe + oneway valve
4 Top part Clear polystyrene
5 Cell surface plates
6 Aeration support
Cell culture plasma-treated clear polystyrene
Polypropylene + polycarbonate
7 Aeration tubing Silicone 0.58 in. ID x 0.77 in. OD
8 Outer tube
9 Stirrer cover
10 Impeller
Polyvinyl chloride
Clear polystyrene
Ultra-high-molecular-weight polyethylene
11 Bearing
12 Bottom part
Polyether ether ketone
Clear polystyrene
13 Liquid in/out tubing
C-Flex Clear 374 Tubing 3/8 in. ID, CPC connector, 0.2 μm hydrophobic filters
Sealing material Resin
◆ MPC male
An Xpansion Plate

Docking Station
The bioreactor docks to a station that includes a magnetic drive stirring unit powering the magnetic impeller.
Control of agitation speed is integrated in the controller. The setpoint is defined via the screen on the front side of the controller.
Controller
The controller has measurement/control hardware and a gassing system with a digital control.
Features of the Xpansion controller include:
Graphic user interface with color display and touch screen operation with Administrator and Operator security levels
Integrated transmitters for temperature, disposable DO and pH sensors
Integrated control loops for DO and pH
Agitation control (speed range from 5 to 250 rpm)
Temperature monitoring
DO and pH sensor patches arrive precalibrated; data are transferred via a USB key to the controller
In-process DO and pH can be recalibrated
Trend display for up to four process values (pH, DO, T, gas flow rate)
Controller Gas Module
The controller is connected to the user’s gas supply line. Its “gas outlet” is connected to the “gas in” filter of the bioreactor. Gas composition to be injected in the bioreactor is regulated by controller solenoid gas valves. The flow rate of the mixed gas is adjusted by a massflow controller (0-200 mL/min). The controller gas module contains:
One gas diffuser outlet
Four solenoid valves of Air, N
2
, O
2
, and CO
2
One Mass Flow Controller for mixed gas flow control
Data Management
The Xpansion system has one ethernet port for data communication/ acquisition. It can be linked to egraph (optional software and computer provided by Pall Life Sciences). egraph is installed on a host PC and provides data acquisition for all process values.
Xpansion Controller and Docking Station
Controller
Height
Depth
Width
Weight
Power supply
Interface
Stirrer speed
Integrated measurement module
One point recalibration function
Gas options
Temperature monitoring
50 cm (19.7 in.)
40 cm (15.7 in.)
25 cm (9.8 in.)
15 kg (33 lbs.)
100 – 240 VAC, 50 – 60 Hz
Ethernet
USB host
5 – 250 rpm
Disposable DO sensor
Disposable pH sensor
Temperature
Disposable DO sensor
Disposable pH sensor
O
2
N
2
CO
2
Air
0 – 50 ºC (32 – 122 ºF)
Gas Requirements
Gases
Air
CO
2
Oxygen
Application Specification pH control Compressed air – medical grade
Outlet pressure between 1 and 2 bars
(14.5 – 30 PSI) 6 mm Festo connectors pH control
DO control
Pure CO
2
Outlet pressure equivalent to air pressure
6 mm Festo connectors
Medical grade oxygen
Outlet pressure equivalent to air pressure
6 mm Festo connectors
Nitrogen DO control
(optional)
Medical grade nitrogen
Outlet pressure equivalent to air pressure
6 mm Festo connectors
3

Standard Cell Environment Preservation
As in multitray stacks, cells adhere and grow on hydrophilized polystyrene plates and are harvested using a cell dissociation reagent.
Because of its specific design, the system maintains the same environment as T-flasks or multitray stacks, and enables implementation of a process easily-adapted from these traditional methods.
Cell Culture Parameters (pH, DO) Control and
Temperature Monitoring
The Xpansion head plate includes patch ports for optical non-invasive measurement of pH and DO. For contamination risk elimination, the determination of pH and DO is based upon the principle of fluorescence. Depending on the DO and pH measurement, gas regulation is activated via the controller according to users’ requirements.
Temperature is measured for monitoring via a PT100 reusable sensor installed in a thermowell of the bioreactor. The heating is done via an incubator or in a warm room.
Each measure (pH, DO and T) is associated with an alarm.
Aeration System
Gas aeration occurs depending on pH and DO control limits set by the users. Gas composition control depends on pH and
DO measurement in the bioreactor. The gas coming from the controller enters via the “gas in” filter of the bioreactor and passes through very thin silicone tubing placed in the central column. The gas inlet and outlet include pre- installed hydrophobic aeration filters. Gases diffuse through the tubing.
Homogenization by Medium Circulation
Medium circulation is coupled with the aeration system which depends on pH and DO control. The medium can also circulate in the bioreactor independent of gas aeration according to users’ requirements. Each plate contains 16 radial channels supporting media flow rate. The rotation of the impeller pushes the liquid upward, first vertically through the first plate channel, then horizontally over the plate, then up again to the second plate, etc.
Cell Morphology and Density Monitoring
Cell density and morphology are monitored via a specialized holographic microscope developed by Ovizio Imaging Systems
NV and available through collaboration with Pall Life Sciences.
This technology provides a platform to observe cells on multiple layers with high image quality.
Optical pH Sensor Performance Specifications
Detection range
Accuracy
Precision
Response time (t90 )
Calibration
Materials pH 6.0 – 9.0
±0.1 @ pH 6.5 – 7.5
±0.01 @ pH 7.0
30 seconds
Shipped pre-calibrated, (calibration files on
USB key provided with the reactor). One point calibration required.
USP Class VI certified
Optical DO Sensor Performance Specifications
Detection range
Accuracy
Precision
Response time (t90 )
Calibration
Materials
0 - 150% air saturation
+/- 3.5% @ DO 15 - 75% air saturation
+/- 1.5% @ DO 15 - 100% air saturation
< 15 seconds
Shipped pre-calibrated, (calibration files on
USB key provided with the reactor). One point calibration required.
USP Class VI certified
The Xpansion Bioreactor Aeration System
Circulation of Culture Medium throughout the Xpansion
Bioreactor Plates
4

Bioreactors Xpansion MPB 10 Xpansion MPB 50
Number of plates
Cell culture surface
Media volume / surface area ratio
Bioreactor volume
10
6,120 cm 2 (949 in.
2 )
0.26 mL/cm 2
Central column volume
Overall weight
1.6 L
26 mL
2.5 kg (5.6 lbs.)
Overall height 29 cm (11.4 in.)
Minimum space requirement 49 cm x 48 cm x 40 cm for the bioreactor and docking station (H x W x D)
(19 in. x 19 in. x 16 in.)
Plate surface 612 cm 2 (94.9 in.
2 )
Plate thickness 0.1 cm (0.04 in.)
Distance between each plate 0.16 cm (0.06 in.)
Volume between each plate 98 mL
Head space volume 50 mL
Sterilization Gamma-irradiated to > 25 kGy
50
30,600 cm 2 (4,743 in.
2 )
0.18 mL/cm 2
5.6 L
132 mL
6.9 kg (15.2 lbs.)
47 cm (18.5 in.)
67 cm x 48 cm x 40 cm
(26 in. x 19 in. x 16 in.)
Xpansion MPB 100
100
61,200 cm 2 (9,486 in.
2 )
0.18 mL/cm 2
11.2 L
263 mL
12.5 kg (27.5 lbs.)
55 cm (21.6 in.)
75 cm x 48 cm x 40 cm
(30 in. x 19 in. x 16 in.)
Xpansion MPB 200
200
122,400 cm 2 (18,972 in.
2 )
0.18 mL/cm 2
21.9 L
527 mL
23.4 kg (50.7 lbs.)
81 cm (31.9 in.)
99 cm x 48 cm x 40 cm
(39 in. x 19 in. x 16 in.)
Category
Hardware
Consumables
Material
Skid
Docking station
Harvest station
D3HM microscope*
Lift* eGraph computer*
Bioreactor
Seeding manifold*
Pooling manifold*
* Optional equipment and consumables
Description
1 controller, 2 optical fibers for pH and DO, 1 temperature probe (PT-100),
1 driver (magnetic stirring plate), 5 M of Festo gas tubing
Bioreactor support for inside incubator
Mechanical cell detachment device for harvest
Holographic; for observation of up to top 10 plates of the system
Ref #
810062 (EU)
810063 (US)
820369
810345SU
Safe movement of bioreactor from package to incubator and during observation under microscope (highly recommended for XP-100 and 200)
Process data recording (pH, DO, temperature and events); 1 computer can be used with up to 10 controllers
XP-10: 10-plate bioreactor
XP-50: 50-plate bioreactor
XP-100: 100-plate bioreactor
XP-200: 200-plate bioreactor
810265 (EU)
810260 (US)
810076 (EU)
810077 (US)
810072
810123
810122
810154
810155
2 L bottle with female MPC ◆ connector used to transfer cell suspension into seeding container. 6100097S
Note: for XP-10 the manifold can be directly connected to the bioreactor; for XP-50,
XP-100 and XP-200, intermediate container must be used.
Sterile 10 L bottle with female MPC connector used for pooling reagent bottles (cell culture medium, PBS, Trypsine, ...) and transfer into Pall 2D BPV bag
6100129
Corporate Headquarters
Port Washington, NY, USA
+1.800.717.7255 toll free (USA)
+1.516.484.5400 phone biopharm@pall.com e-mail
European Headquarters
Fribourg, Switzerland
+41 (0)26 350 53 00 phone
LifeSciences.EU@pall.com e-mail
Asia-Pacific Headquarters
Singapore
+65 6389 6500 phone sgcustomerservice@pall.com e-mail
Visit us on the Web at www.pall.com/bioreactors
E-mail us at bioreactors@pall.com
International Offices
Pall Corporation has offices and plants throughout the world in: Argentina, Australia, Austria,
Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan,
Korea, Malaysia, New Zealand, Norway, Philippines, Poland, Russia, Singapore, South
Africa, Spain, Sweden, Switzerland, Taiwan, Thailand, United Kingdom, and Vietnam.
Distributors in all major industrial areas of the world. To locate the Pall office or distributor nearest you, visit www.pall.com/contact.
The information provided in this literature was reviewed for accuracy at the time of publication.
Product data may be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
© 2015, Pall Corporation. Pall, , and Xpansion are trademarks of Pall Corporation. ® indicates a trademark registered in the USA and TM indicates a common law trademark. Filtration. Separation. Solution.
is a service mark of Pall Corporation. ◆ CPC and MPC are trademarks of Colder Products Company; C-Flex is a trademark of Cole-Palmer
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