Roots, Stems & Leaves Chapter 23 Overview of the Cell Nucleus • The nucleus contains nearly all of the cell’s DNA, which includes all the instructions for making proteins and other important molecules. Also contains a structure called the nucleolus, where ribosomes are produced. Cell Membrane • The cell membrane (or plasma membrane) regulates what enters and leaves the cell, and provides protection and support. Many substances can pass through the cell membrane, through active or passive transport. Cell Wall • The cell wall (found in plants, algae, and fungi, but not in animals) lies outside the cell membrane. The main function of the cell wall is to provide support and protection for the cell. Plant cell walls are made mostly of cellulose. Most cell walls are porous enough to allow transport of some materials in and out of the cell. Cell Wall Cytoplasm • The portion of the cell outside the nucleus is the cytoplasm or plasma. The cytoplasm includes all of the specialized structures that carry out specific tasks within the cell. These structures are known as organelles. • The main organelles are: Ribosomes • Ribosomes are small particles of RNA and protein where the coded messages from DNA are used to produce proteins. Endoplasmic Reticulum (ER) • The ER is an internal membrane system where lipid components of the cell membrane are assembled. • Some ER is covered in ribosomes, and the proteins produced by those ribosomes are moved into the ER to be modified. This type of ER is known as rough ER because of its appearance. • Other portions of the ER do not have ribosomes, and is known as smooth ER. This smooth ER often contains specialized enzymes to perform specific tasks. Rough & Smooth ER Golgi Apparatus • The Golgi apparatus functions to modify, sort, and package proteins and other materials from the ER, for storage in the cell or export from the cell. Lysosomes • These are small organelles filled with enzymes, they are designed to digest or breakdown lipids, carbohydrates, and proteins into small molecules that can be reused by the cell. • Lysosomes perform the vital function of cleaning up “junk” in the cell, including breaking down old organelles and other items that would clutter up the cell. Vacuoles • These are saclike structures that store materials such as water, salts, proteins, and carbohydrates. • Plant cells often contain a central vacuole filled with liquid. This central vacuole helps to maintain the structure of the plant through turgor pressure. Central Vacuole Mitochondria • These are organelles that convert stored chemical energy from food into compounds that can be use immediately by the cell for energy. • Mitochondria are known as the “powerhouse” of the cell. Chloroplasts • Chloroplasts – these are organelles that capture the energy from sunlight and convert it into chemical energy through photosynthesis. Label Your Diagram Label Your Diagram Colour-Code your Diagrams • Pick a colour for each type of organelle or structure, use it to underline the term in your notes and on the diagram – Then colour in that structure in that colour • If the structure is found in both types of cells, then use the same colour for both! • This diagram will go into your logbook • I’ll play a couple of summary videos while you colour Crash Course - Cells • Eukaryotic cells (animal cells) https://www.youtube.com/watch?v=cj8dDTH GJBY (11:34) • Plant cells • https://www.youtube.com/watch?v=9UvlqAV CoqY (10:30) Seed Plant Structures • Roots – Roots absorb water and dissolved nutrients and transport them to the rest of the plant. Roots also anchor the plant in the soil and hold plants upright against wind and rain. • Stems – Stems include a support system, a transport system, and a defense system. • Leaves – Leaves are the main site of photosynthesis – the broad surfaces allow for effective light absorption, but also make water loss an issue, so leaves also need to contain systems to reduce water loss. Plant Tissue Systems • Plants contain 3 main tissue systems – Dermal tissue, vascular tissue, and ground tissue. Dermal Tissue • Dermal tissue is like the skin of a plant, it consists of a single layer of cells called epidermal cells. These cells are often covered in a waxy coating (the cuticle) to protect against damage and prevent water loss. Dermal Tissue • Dermal cells sometimes have tiny projections called trichomes, which protect the leaf (this is also why leaves sometimes look fuzzy). • On the bottom of leaves, the dermal tissue includes guard cells to regulate water loss and gas exchange through the stomata. • In roots the dermal cells include root hairs which increase the surface area for water absorption. Vascular Tissue • Vascular tissue makes up a transport system to move water and nutrients throughout the plant. • The main subsystems are the xylem (waterconducting tissue) and phloem (foodconducting system). • Both these systems are made up of systems of hollow, specialized cells that can carry fluids through the plant body. Xylem • Xylem is made up mostly of cells called tracheids; long narrow cells with cells walls that are impermeable to water. There are openings in the cell walls to connect neighbouring cells together. • These cells mature and die, and their cytoplasm disintegrates, leaving a series of hollow cells that are interconnected. Vessel Elements • Angiosperms have an additional type of xylem cell, called a vessel element. • They also mature and die, leaving hollow cells behind, but they are much wider than tracheids, and the cell walls at their ends are lost when the cell dies, creating a continuous tube, through which water can flow easily. Phloem • Phloem is made up mostly of cells called sieve tube elements. These cells are placed end to end, like vessel elements, to form sieve tubes. • The end walls have many small holes in them. As sieve tube elements mature, they lose their nuclei and most other organelles. Any remaining organelles stick to the side walls. • The cell is then mostly cytoplasm, and sugars and other substances can be easily transported from one cell to another through the holes in the end walls. Phloem – Companion Cells • Phloem also contains companion cells, which surround sieve tube elements. These cells keep their nuclei and other organelles, and they support the phloem cells and help to move substances into and out of the phloem. Ground Tissue • All the cells between the dermal tissue and the vascular tissues are known as ground tissue. In most plants, the main cells in the ground tissue are parenchyma cells. Parenchyma Cells • These cells have thin cell walls, large central vacuoles and a thin layer of cytoplasm. In leaves these cells are packed with chloroplasts, and are the site where most photosynthesis takes place. Ground Tissue • There are 2 other types of cells found in ground tissue, both with thicker cell walls. • Collenchyma cells have strong, flexible cell walls that help to support larger plants. • Sclerenchyma cells have extremely thick and rigid cell walls to make the ground tissue tough and strong. Plant Growth & Meristematic Tissue • Plants produce new cells at the tips of their roots and stems for as long as they live. These new cells are produced at meristems – clusters of tissue that produce new cells through mitosis. A meristem that is found at the tip of each growing root or stem is called an apical meristem. Meristems • The new cells produced by the meristems are initially undifferentiated (not yet specialized). As they mature, they take on specialized structures and functions to become part of particular tissuetypes. This process is called differentiation. Floral Meristems • In angiosperms, certain shoot apical meristems can have particular genes turned on, and they change into floral meristems. A floral meristem produces both the modified leaves that become the petals, and the reproductive tissues of the flower. Celery Demonstration • I’ll set it up, fill in your predictions • We’ll fill in the “before” masses • We’ll check it again next week to see what has happened. Roots • When a new seed germinates, it produces its first root to absorb water and nutrients from the soil, then other roots branch out from the first to increase the surface area. The total surface area of the roots can be much larger than that of the stems and leaves. Types of Roots • There are two main types of roots • Taproots – found mostly in dicots – The primary root grows long and thick, any secondary roots remain small – Some tap roots of trees can grow long enough to reach water in aquifers – Examples: carrots, dandelions, beets, radishes Types of Roots • Fibrous roots – found mostly in monocots – No single root grows larger than the others, there is extensive branching – These roots help to prevent topsoil from washing away in heavy rain – Examples: grasses, coconut palm Types of Roots Root Structure • Roots contain cells form all three tissue layers: dermal, vascular, and ground tissue. Dermal System • The epidermal system of the root has two functions, protection & absorption – Covered in cellular projections called root hairs • Penetrate the spaces between soil particles and increase the surface area for water absorption • There’s also an endodermal layer between the ground tissue and the vascular tissue – Completely surrounds the vascular cylinder Ground Tissue • A spongy layer of ground tissue called the cortex is sandwiched between the epidermis and endodermis Vascular Tissue • The vascular cylinder of xylem and phloem allows water, nutrients, and sugars to be transported into and out of the roots. Root Growth • The root grows as the root apical meristem produces new cells near the root tip. Most of the increase in root length is produced at this meristem, as new undifferentiated cells elongate and then differentiate into their specialized cell types. Root Growth • These new cells are fragile and would be damaged when pushing through the soil, so they must be protected by a tough root cap. The root cap also produces a slippery secretion to help the root move through the soil more easily. Cells on the root cap are continuously scraped away and must be replaced regularly. Amazing Plant Roots • https://www.youtube.com/watch?v=_jVyhSe5 pvk (3 min) Root Functions • Water does not just “soak” into the root from the soil - the plant has to spend energy to absorb water. Soil • Soil is a complex mix of sand, silt, clay, air, and bits of decayed matter from plants and animals. Depending on the location and depth, soil will have a different mix of these ingredients. Sandy soils are low in nutrients, while silty or clay soils with more organic matter are higher in nutrients. Essential Nutrients • In addition to water and carbon dioxide, plants require a variety of essential inorganic nutrients such as phosphorus, nitrogen, potassium, magnesium and calcium. See below for the role of each nutrient. These nutrients are found in varying amounts in the soil, and are drawn up through the roots. Trace Elements • Lastly, plants also require trace elements in very small quantities for proper plant growth (although large amounts of these trace elements can be poisonous to the plant). Trace elements include sulfur, iron, zinc, molybdenum, boron, copper, manganese, and chlorine. Essential Plant Nutrients Nutrient Role in Plants Result of Deficiency Nitrogen Proper leaf growth and colour; synthesis of Stunted plant growth, amino acids, proteins, nucleic acids (for pale yellow leaves DNA and RNA), and chlorophyll Phosphorus Synthesis of DNA; development of roots, stems, flowers, and seeds Potassium Synthesis of proteins and carbohydrates; Weak stems and stunted development of roots, stems, and flowers; roots; edges of leaves turn resistance to cold and disease brown Magnesium Synthesis of chlorophyll Calcium Cell growth and division; cell wall structure; cellular transport; enzyme action Poor flowering, stunted growth Thin stems; mottled, pale leaves Stunted growth; curled leaves Homework • Start your Glossary – Find all of the BOLD TERMS in your textbook for Chapters 22-24 • Those are your glossary terms! • You need to define these in your own words for your logbook (Due Jan 9th) Unit Test Thursday Dec 18th On all of Chap 22 &23 (Chap 24 will be after the break) Active Transport of Minerals • The cell membranes of epidermis cells in the roots contain active transport proteins. These proteins use ATP (the quick-energy source produced by the mitochondria) to pump mineral ions from the soil into the plant. Water Movement • Moving these ions into the plant produces a difference in concentration inside and outside of the root. • Osmosis is the movement of water to equalize the concentrations on each side of a membrane. Therefore water “follows” the mineral ions into the root via osmosis. • Notice that the root does not actually “pump” in water – it pumps in ions, and water simply follows via osmosis to try to equalize the concentrations. Osmosis Video • https://www.youtube.com/watch?v=eQsAzXr 0UCU (4:30) Into the Vascular Cylinder • Once the minerals and water have moved into the cortex, then they pass the inner boundary of the cortex and into the endodermis. • The endodermis completely surrounds the vascular tissue of the root, creating a cylinder. • The endodermis is ringed by a water-proof strip called the Casparian strip – this prevents the backflow of water from the vascular cylinder back into the root cortex. Osmosis • Water moves into the vascular cylinder by osmosis. Water and minerals cannot pass back through the Casparian strip, so they become trapped in the vascular cylinder once they pass through the endodermis. This produces a one-way movement of these materials into the vascular cylinder of plant roots. Root Pressure • This one-way movement of water and minerals into the vascular cylinder is necessary because it produces the pressure needed to move water up and into the rest of the plant. • As minerals are pumped into the vascular cylinder, more and more water follows, creating a strong pressure. Root Pressure • Since the Casparian strip prevents the water from flowing back, this pressure can only go upward into the xylem, and through the xylem to the rest of the plant. • This is known as root pressure, and it is the starting point for the movement of water through the vascular system of the plant. We will learn about the other processes when we cover stems and leaves. Stems • Stems have three key functions – to produce leaves, branches, and flowers; to hold up the leaves to access sunlight; and transporting substances between the roots and the leaves. Stems • Stems are made up of all 3 types of tissue. They are surrounded by a layer of epidermal cells, which have thick cell walls and a waxy protective coating. • The xylem and phloem form continuous vascular tubes to link the leaves with the roots and all parts of the plant. • Stems can also function as storage systems in some plants, and some also participate in photosynthesis. • Leaves are attached to the stem at locations called nodes, and the regions between these nodes are called internode regions. Buds form at these nodes – buds are made of undeveloped tissue that can produce new stems and leaves. Dicot & Monocot Stems • A stem cut in cross-section shows the three tissue layers clearly, but they look quite different in monocots and dicots. Monocot Stems • Distinct epidermis on the outside • Ground tissue is fairly uniform, made up of mostly parenchyma cells • There is a series of vascular bundles, each made up of xylem & phloem – Scattered throughout the ground tissue – Phloem faces the epidermis (outside of the stem) – Xylem faces the centre of the stem Dicot Stems • Distinct epidermis on the outside • Vascular bundles (made up of xylem and phloem) are arranged in an orderly ring • Ground tissue is in two sections – Pith – parenchyma cells inside the ring of vascular bundles – Cortex – parenchyma cells outside the ring – between the vascular tissue and epidermis Primary Growth • The growth of a plant at the ends of its stems and roots by the apical meristems is called primary growth. • This produces an increase in length, but not any increase in width. • Each year a new length of stem is added through this primary growth, which can be seen easily on a tree branch. Secondary Growth • Plant stems cannot simply increase in length forever – they must also increase in thickness. • This increase in mass also means they need more vascular tissue to transport water and nutrients. This type of growth is called secondary growth. • How can plants grow wider, when only meristem tissue can produce new cells? Monocots & Dicots • Some monocots (including palm trees) can grow thick stems from a meristem that grows wider as the plant grows. However most monocots don’t grow to be very tall or large. • Dicots can grow to be extremely tall, and they must grow wider to support the extra weight. Lateral Meristems • In conifers and dicots, secondary growth occurs in lateral meristem tissues called the vascular cambium and the cork cambium. – Vascular cambium produces new vascular tissues – Cork cambium produces the outer coating of stems – A separate type of cambium allow roots to grow thicker and create new branching roots Cambium Vascular Cambium • In a young dicot stem produced by primary growth, the vascular bundles are found in a ring. • A vascular cambium appears as a thin layer between the xylem and phloem of each vascular bundle. • This meristematic tissue can divide to produce new cells to add to the vascular bundles. As a result, the stem becomes thicker and thicker. Homework • Read Section 23-3 (pg 589-594) – Questions 1-4 pg 594 • Start your Glossary – Find all of the BOLD TERMS in your textbook for Chapters 22-24 • Those are your glossary terms! • You need to define these in your own words for your logbook (Due Jan 9th) Unit Test Thursday Dec 18th On all of Chap 22 &23 (Chap 24 will be after the break) Formation of Wood • Most of what we call wood is actually layers of xylem. These cells build up year after year as the stem grows thicker. The older xylem near the centre stops conducting water, and becomes heartwood. Heartwood becomes darker over time because of accumulated impurities. Heartwood & Sapwood • Heartwood is surrounded by sapwood. This sapwood is still active in transporting water and sap, so it is usually lighter in colour. Seasonal Growth • In the temperate zone, tree growth is seasonal. • During the spring when growth begins, the vascular cambium grows quickly, producing large, lightly-coloured xylem cells with fairly thin cell walls. – This produces a light coloured wood called “early wood”. • Later in the growing season the rate of growth slows, and cells become smaller and have thicker cell walls. – This produces a thin layer of darker wood known as “late wood”. Early Wood and Late Wood Tree Rings • This alternation of light and dark wood in each year’s growth produces what we usually call tree rings. • By counting these tree rings you can estimate the age of a tree. • These rings can also tell us about the weather conditions during that year – wide rings indicate years with favourable growing conditions, whereas thin rings indicate years with poor growing conditions. Tree Rings Formation of Bark • The bark on most trees is made up of all of the tissues outside the vascular cambium – including phloem, cork cambium, and cork. Process of Forming Bark • As a tree lays down new xylem, it expands in width, which forces the phloem outward • If the phloem does not grow as well, this could lead to splitting of the epidermis • The cork cambium (another layer of meristematic tissue) surrounds the cortex and produces a thick layer of protective cork Cork • Cork is make up of cells with thick cell walls • These cork cells usually contain fats, oils, or waxes – These substances are waterproof and help prevent the loss of water from the stem • Outer cork cells are usually dead, and as the stem grows in size this cork often cracks and flakes off in patches or strips Layers of Wood & Bark Modified stems • Many plants have modified stems that have been adapted to store food and to remain dormant while waiting for favourable conditions. • Some examples include tubers, bulbs, rhizomes and corms. Tubers • A tuber is a section of underground stem that stores food for the plant. Potatoes and yams are common examples. Bulb • A bulb is made of a central stem surrounded by thick, short leaves. The leaves wrap around to protect the stem and also store food. Bulbs can remain dormant for a long time, and then grow into a new plant when conditions are right. • Common examples include tulips, onions and garlic. Bulb Rhizomes • Rhizomes are horizontal underground stems, from which new plants can grow after long periods of dormancy. • Ginger and turmeric are rhizomes, and plants such as bamboo, asparagus and lily of the valley use rhizomes to spread asexually. Corms • Corms look like bulbs, but instead of being a combination of stem and leaves, a corm is a thickened stem that stores food. • Taro, crocuses and gladiolus plants produce corms. Svalbard Seed Vault • https://www.youtube.com/watch?v=Mm8hsU XJ8Jk&list=PL4LEUrNDNoyRQqVGWYpMS_lbg2HLQjcm&index=9 (3 min) Celery Demonstration • General Observations – How have the 4 stalks changed since last week? – Can you tell which one has pulled up the most water? • Masses – The sub will use the scale to weigh the 4 stalks • Careful not to wipe off the vaseline! – Record these “after” values in your chart • Finish up the results/analysis/discussion sections for homework – this will go into your logbook. Review for Unit Test on Thursday • Start Chapter Reviews from Textbook: – Chapter 22: 574-575 – Chapter 23: 604-605 • Mrs. Mishra will give you the topics list and more information tomorrow. • You can work on these while we watch this summary episode of the Magic School Bus – https://www.youtube.com/watch?v=W8nfkS40u Wc (25 min)