Moving Through the Plasma Membrane Let’s Review • What is homeostasis? • What is the job of the plasma membrane? • How do you think the cell membrane helps a cell maintain homeostasis? Structure and Function • Phospholipid bilayer with carbohydrates and proteins imbedded into itself • Brings in essential materials and excretes waste products What does a phospholipid look like? Polar head/ hydrophilic Nonpolar head/ hydrophilic What does the Plasma Membrane look like? Outside Cell (Extracellular Matrix) Protein Glycoprotein Cholesterol Inside Cell (Cytoplasm) Phospholipids The Plasma Membrane Polar Non-Polar Outside Cell (Extracellular Matrix) Protein Glycoprotein Cholesterol Inside Cell (Cytoplasm) Phospholipids Plasma Membrane: The Fluid Mosaic Model Plasma Membrane: The Fluid Mosaic Model • Fluid because –the phospholipid and protein molecules are able to move around. Not stuck in one place. • Mosaic because – the membrane contains a variety of proteins embedded with the phospholipids. How do particles move in and out of the cell? Permeability of a membrane • Permeable: anything can pass through • Semi-permeable: some things can pass through • Impermeable: nothing can pass through • The plasma membrane is described as semi-permeable because some substances can move through it: –Small and –Non-polar Passive Transport energy is • No additional _______________ required because every particle has its own energy • This energy produces random movement in ________________ particles. (a.k.a Brownian motion) Diffusion • Diffusion is the movement of high substances from a ________ concentration to a low concentration. • Diffusion is caused by Brownian Motion ______________________ • Concentration is the amount _________ of area something in a given ______. Examples of diffusion at work • Why does food smell fragrant when cooked? • Have you ever stood next to someone wearing strong perfume? How does it work? • Particles move down the concentration gradient, until equal the concentration is _________ throughout an area. • Then the system is said to have reached dynamic equilibrium. A. High Concentration of Blue Dots High Concentration of Orange Dots Low Concentration of Orange Dots Low Concentration of Blue Dots B. System has reached dynamic equilibrium Back to the plasma membrane… Outside Cell (Extracellular Matrix) Inside Cell (Cytoplasm) Osmosis • Diffusion of _________ water across a membrane • Water molecules can pass through pores or openings created by proteins in the plasma membrane . __________ Back to the plasma membrane… Outside Cell (Extracellular Matrix) Protein Glycoprotein Cholesterol Inside Cell (Cytoplasm) Phospholipids The Cell’s Environment: Isotonic • In an isotonic environment the solute concentrations equal and there is no are ___________ NET movement of water. The Cell’s Environment: Hypotonic • In a hypotonic environment, there are less solutes outside __________ than inside and water moves into the cell. The Cell’s Environment: Hypertonic • In a hypertonic environment there are more solutes outside _________ than inside and water moves out of the cell. Environment Water outside cell moves… Hypotonic Hypertonic Isotonic Effect on Effect on ANIMAL cell PLANT cell Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic Swells, then bursts (cytolysis) Hypertonic Isotonic In Animal Cell Cytolysis Cells burst Cell still intact Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic Isotonic In Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic Out Isotonic Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic out Shrinks (crenation) Isotonic Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic out Shrinks Shrink (crenation) (plasmolysis) Isotonic Environment outside cell Water moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then bursts (cytolysis) Hypertonic Out Shrinks (crenation) Isotonic Swells, increased turgor pressure Shrinks, (plasmolysis) Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic out Shrinks Shrink (crenation) (plasmolysis) Isotonic Plant cell: Plasmolysis Plasmolyzed Cells Normal cells Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic out Shrinks Shrink (crenation) (plasmolysis) Isotonic In and out at the same rate Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic out Shrinks Shrink (crenation) (plasmolysis) Isotonic In and Stays the out at same the same rate Environment Water outside cell moves… Effect on Effect on ANIMAL cell PLANT cell Hypotonic In Swells, then Swells, bursts increases (cytolysis) turgor pressure Hypertonic out Shrinks Shrink (crenation) (plasmolysis) Isotonic In and Stays the out at same the same rate Stays the same Osmosis in Action • What happens when you sprinkle salt on a slug? • If you are stranded at sea and run out of water, should you drink sea water? ** Water moves from a hypo to a hypertonic environment. True or False 1. In diffusion, particles move from an area of high to low concentration. 2. Diffusion does not require any additional energy to occur. 3. Small, charged or polar particles can move through the cell membrane by simple diffusion. What if a cell needs large or charged/ polar molecules. Can they move across the membrane? Yes! Selectively Permeable Membrane • The cell membrane “picks” what molecules can enter and exit the cell because proteins in the membrane allow specific macromolecules or ions in or out of the cell. Facilitated diffusion • Proteins help particles move across the membrane • Transport proteins span the phospholipid bilayer, but allow only ___________specific molecules through. >> Selectivity Types of transport proteins Ion __________ channels are non–polar on the • ____ outside and polar on the inside. They provide a pore for ions and polar particles to move through. Carrier_______________ Proteins • _________ bind to specific particles, carry them through the membrane, and release them on the other side. http://www.stolaf.edu/people/giannini/flashanimat/transport/caryprot.swf Remember! • In diffusion-particles (solutes) move from an area of HIGH concentration to LOW In osmosiswater moves from a HYPOTONIC environment to a HYPERTONIC environment • Diffusion requires NO energy input • http://scienceguyinatie.blogspot.com/2008/11/cell-membrane-animation-of-word-wall.html Simple vs. Facilitated • In simple diffusion, particles move between phospholipid molecules of the membrane. • In facilitated diffusion, particles move through transport proteins in the membrane. • Both do NOT use energy. Particles must move down the concentration gradient. But what if you needed to move particles against their concentration gradient? Active Transport • Transport proteins use ________to energy move particles against their concentration gradient, from a low concentration to a high concentration. Sodium-Potassium Pump: • Carrier- protein that pumps 3 sodium ions (Na+)________ outside the cell, while pumping 2 potassium ions (K+)________. inside http://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_the_sodium_potassium_pump_works.html http://www.brookscole.com/chemistry_d/templates/student_resources/shared_resources/animations/ion_pump/ionpump.html 1. 3 Na+ ions from inside the cell bind to the pump protein. Energy from ATP is added to the protein. 2. The Na+ ions are moved to the outside of the cell. 3. The 3 Na+ ions are released and 2 K+ ions bind to the protein. 4. The protein changes back and releases the 2 K+ ions into the cell. Vesicle Mediated Transport • The fluid plasma membrane can “pinch” off forming vesicles that can move very large _______particles or lots of small ______particles. energy • This process needs _______. Endocytosis • Outside materials are brought inside the cell. __________ 1) Food particle 2) Pouch forming around food 3) Vesicle with food Endocytosis Phagocytosis • “Cell eating” • Cell engulfs large food particles and the vesicle is a food vacuole. • http://www.stolaf.edu/people/giannini/flashanimat/cellstructures/phagocitosis.swf Pinocytosis • “cell drinking” • Cell engulfs small droplets of surrounding fluid. Exocytosis • Material from inside the cell is outsideby vesicles. released _________ 1) Vesicle fuses with membrane 2) Pouch opens 3) Particle released