SI Bios 1700
10.30.14
Describe or illustrate what would occur if a cell were to lose all function in the
following enzymes.
1. RNA primase
2. DNA ligase
3. Helicase
1. Which DNA polymerase acts exclusively on the lagging strand?
2. Which of the following are DNA molecules? If a given molecule is not DNA,
note what it is.
a. DNA replication primer
b. Okazaki fragment
c. Origin of replication
d. Telomere
e. Telomere template in telomerase
3. What is an ‘origin of replication’? How does this differ between eukaryotic
and prokaryotic organisms?
4. Sketch replication of a section of eukaryotic DNA including at least two
origins of replication.
5. Why does the human genome contain telomeres? Where are these located?
Compare and contrast telomerase activity at the 5’ and 3’ ends of the
template.
6. Supposing that telomerase carries the RNA sequence 5’ CCCUAACCC 3’,
explain how it can lengthen the genome by six base pairs each time it forms a
complex with DNA. (Draw it!)
7. What will occur if telomerase activity is deactivated?
8. List the components required for PCR. What will occur if you leave each of
these out?
9. List the three steps of PCR and explain what happens in each.