Cellular Transport SBI4U Biochemistry Concept Presentation By: Kasia Hirniak Background Knowledge http://www.youtube.com/watch?v=4c1r8ydVuds Curriculum Expectations Overall Expectation B2. investigate the chemical structures, functions, and chemical properties of biological molecules involved in some common cellular processes and biochemical reactions Curriculum Expectations Specific Expectations B2.2 plan and conduct an investigation to demonstrate the movement of substances across a membrane (e.g., the effects of salt water and distilled water on a potato). B3.6 describe the structure of cell membranes according to the fluid mosaic model, and explain the dynamics of passive transport, facilitated diffusion, and the movement of large particles across the cell membrane by the processes of endocytosis and exocytosis. Advance Preparation Students should have a good understanding of the following concepts: Cell structure and function Molecules are always in motion Cell permeability Cells store and use energy for building materials, transport and controlling life processes Cells need chemical compounds to perform functions and build structures The law of diffusion Student Difficulties Student Difficulty •Students may have difficulty learning to think about the processes at the molecular level. •Students may believe that in diffusion when the molecules are evenly spread out they stop moving. •Students may confuse osmosis and diffusion by thinking they are the same. •Students may confuse diffusion with dissolving. •Students may find the concept of tonicity (isotonic, hypotonic, hypertonic) difficult to understand. They may have hard time remembering which direction water moves in each of the three types of solutions. Rectification Showing students animations and videos can help them visualize how the processes work. Engaging students in a lab or showing them a demonstration can help them differentiate between different processes. Lesson Sequence Lesson 1: The Structure and Composition of the Cell Membrane Lesson 2: Passive and Active Transport Lesson 3: Cellular Transport Stations Lesson 1: Cell Membrane Introductory Group Activity: Building a model of the cell membrane using metal paper fasteners Topics Covered: • • • • Properties of lipids Structure of the phospholipid molecule Structure of the cell membrane Components of the cell membrane Assessment: Fluid Mosiac Model Video: http://www.youtube.com/watch?v=ULR79TiUj80 Journal Entry Lesson 2: Active and Passive Transport Topics Covered: • • Passive Transport: Diffusion, Osmosis, Facilitated Diffusion Active Transport: Endocytosis, Exocytosis Teaching Tools: • Introductory Salty Potato Demonstration • Osmosis Gizmo Activities • Egg Osmosis Lab • Active Transport Video Assessment: http://www.youtube.com/watch?v=0c8acUE9Itw Gizmos Worksheets/ Lab Report Lesson 3: Cellular Transport Stations Task: Students will be divided into groups and will rotate around the classroom visiting stations and completing Cellular Transport activities Assessment: Teacher Checklist Self/Peer Evaluation Quiz Station 1: Building a Model of the Cell Membrane Materials: Cotton Swabs Pipe Cleaners Drinking Straw Station 2: Labeling Task: Students label the following components of the cell membrane Phospholipids Glycoproteins Cholesterol Hydrophobic fatty acids Protein channels Station 3: Cell Transport Classification Task: Students indicate the type of transport illustrated on pictures provided. Station 4: How do specific substances cross the cell membrane? Task: Students predict the mode of transport of the following substances Oxygen Neutotransmitters Pepsin Glucose Virus Sodium Ions Applications The effect of Marathon runners drinking fluids that contain sugar and salt. The reason for gardeners to soak fresh vegetables from the garden in saltwater before rinsing them and soaking them in freshwater. Grocery stores misting vegetables to preserve their freshness. Osmosis being vital to many body processes, including: blood filtration by the kidneys and the workings of the nerves. The importance of diffusion in cellular respiration, wherein cells take in oxygen and push out waste carbon dioxide. Malfunction of the chlorine channel causes cystic fibrosis. The secretion of insulin from pancreatic cells into the blood by exocytosis to lower blood sugar level preventing diabetes. Build-up of cholesterol in cell membranes leading to atherosclerosis. Annotated Internet Addresses/References http://science-class.net provides resources for elementary and middle school science teachers, lessons/slideshows, labs, simulations/videos, student worksheets/handouts, quizzes, study notes, review games etc http://home.earthlink.net/~shalpine/anim/Life/memb.htm an interactive demonstration of the structure and function of the cell membrane. http://www.johnkyrk.com/diffusion.html a simple animation showing a high concentration of molecules in the center of the page. As you watch, they will diffuse through random molecular motion. http://www.wisc-online.com/objects/index_tj.asp?objid=AP11003 a tutorial that will help understand the process of osmosis. http://www.wisc-online.com/objects/index_tj.asp?objid=AP11203 a learning activity showing endocytosis, exocytosis, and active transport. http://www.youtube.com/watch?NR=1&v=Kd3LorZcJew video showing the sodium/potassium pump as an example of active transport