COURSE OUTLINE
ERT 317Kejuruteraan Biokimia / Biochemical
Engineering
Semester 1
2015/2016
Nilai unit/ Number of unit:
4
Jenis kursus/ Course type: Teras / Core
Prasyarat/ Prerequisite:
ERT 106 Biochemistry
Sinopsis kursus / Course synopsis:
This course focuses on the importance of bioprocess
engineering elements such as enzyme technology,
fermentation and bioconversion technology. Mathematical
representations of enzymatic reaction as well as microbial
systems are featured among lecture topics especially on
kinetics study.
Hasil Pembelajaran / Learning Outcomes:
At the end of the course, students are expected to be:
Ability to apply knowledge and solve engineering problem
related in developing enzyme reactions based on its kinetics study
and applied catalysis.
Ability to identify, formulate, analyze and design solutions
complex engineering problems using mathematics principles,
natural sciences and engineering sciences for batch and continuous
microbial systems, metabolic pathways and kinetics study.
Ability to propose bioconversion technologies of microbial,
plant and animal cell culture for the production of value-added
product and defend it through verbal presentation in a group.
Senarai eksperimen / List of experiments:
• Lab 1: To analyze the effect of enzyme and substrate
concentration on enzyme kinetics study
• Lab 2: To analyze the effect of immobilization method on the
retention of enzymatic activity
• Lab 3: To analyze the effect of environmental conditions on
growth kinetics of S. cerevisae in batch process
• Lab 4: Effect of cell immobilization on dye removal by
bioabsorbent
• Lab 5: To organize an open-ended experiment based on
bioconversion technology approaches
Pendekatan pembelajaran / Learning
approach:
• (i) Lecture = 42 hours
• (ii) Laboratory = 10 hours
• (iii) Tutorial = 12 hours
• (iv) PBL = 9 hours
Sumbangan penilaian / Evaluation contribution:
Peperiksaan/ Examination: 70 %
• Mid Term Examinations
• Final Examination
= 20 %
= 50 %
Penilaian Berterusan/ Continual Assessment: 30 %
• Quizzes/Assignment
• Lab report
• Integrated Project
=10 %
=10 %
=10 %
Senarai buku teks dan rujukan / List of text books and references :
• Shuler, M. L. and Kargi, F. (2014). Bioprocess Engineering: Basic Concept.
2nd Ed. Upper Saddle River, NJ: Prentice Hall PTR
• Rao, D.G. (2010). Introduction to Biochemical engineering. 2nd Ed. Tata
McGraw Hill Education Private Limited
• Dutta, R. (2008). Fundamentals of Biochemical Engineering. New Delhi:
Ane Books India
• Katoh, S. and Yoshida, F. (2009). Biochemical Engineering: A Textbook for
Engineers Chemists and Biologists. Weinheim: Wiley-VCH Verlag GmbH &
Co
• Nielsen, J., Villadsen, J. and Gunner L. (2011). Bioreaction Engineering
Principles. 3rd Ed. New York: Springer Science+Bussiness Media
RANCANGAN MENGAJAR/LESSON PLAN
Minggu/Week
Kandungan Kursus / Course Contents
(Panduan/Guidelines)
Tugasan
Bacaan/Reading
Assignment
Pensyarah/Lec
turer
CHAPTER 1: ENZYME KINETICS AND APPLICATIONS
Chapter 1.1: Kinetics of Enzyme Catalyzed Reactions
Week 1 - 2
(7/09 – 18/09)
Introduction - Classification of enzymes according to the reaction
catalyzed and how enzymes work.
Enzyme kinetics - Mechanistic model for simple enzyme kinetics,
determination of rate parameters for Michaelis-Menten kinetics;
models for more complex enzyme kinetics; effects of pH and
temperature and insoluble substrates
Chapter 1
Mrs. Hafiza
Shukor
CHAPTER 1: ENZYME KINETICS AND APPLICATIONS
Mrs. Hafiza
Shukor
Chapter 1.2 : Applied Enzyme Catalysis
Week 3
(21/09 – 25/09)
Immobilized enzyme systems - Methods of immobilization,
diffusional limitations in immobilized enzyme systems, electrostatic
and steric effects in immobilized enzyme systems
Enzyme production and application – Large scale production of
enzymes, medical and industrial utilization of enzymes
Chapter 1
CHAPTER 2: MAJOR METABOLICS PATHWAY
Week 4 - 5
(28/9 – 9/10)
Introduction to metabolism and bioenergetics. Glucose
metabolism: Glycolysis and TCA cycle, respiration. Metabolism of
nitrogenous compounds and hydrocarbons. Overview of
biosynthesis for small and macromolecules, anaerobic and
autotrophic metabolism.
Mrs. Hafiza
Shukor
Chapter 2
CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS
CULTURES
Week 6 - 7
(12/10 – 23/10)
Chapter 3.1 : Kinetic of Growth in Batch Culture
Quantifying cell concentrations, growth patterns and kinetics in
batch culture, effects of environmental conditions on growth
kinetics, heat generation by microbial growth.
Chapter 3
Dr. Saleha
CHAPTER 3 : KINETIC OF GROWTH IN BATCH AND CONTINOUS CULTURES
Week 8-9
(26/10 – 6/11)
Week 10
(9/11 – 13/11)
Week 11 (16/11 –
20/11)
Chapter 3.2 : Kinetic of Growth in Continuous Culture
Some specific devices for continuous culture, derivation for ideal
chemostat, application of chemostat and its deviation of ideality.
Chapter 3
Dr. Saleha
Cuti Pertengahan Semester / Mid-semester Break
CHAPTER 4: STOICHIOMETRY OF MICROBIAL GROWTH AND PRODUCT
FORMATION
Overall growth stoichiometry and design medium formulation,
stoichiometric calculation of elemental balances and degree of
reduction, theoretical predictions of yield coefficients.
Chapter 4
Dr. Saleha
Chapter 5
Dr. Saleha
Chapter 6
Dr. Saleha
CHAPTER 5 : BIOPROCESS CONSIDERATIONS IN USING ANIMAL AND
PLANT CELL CULTURES
Week 12-13
(23/11 – 4/12)
Chapter 5.1 : Considerations in Using Animal Cell Culture
Structure and biochemistry of animal cells, methods used for the
cultivation of animal cells, products of animal cell cultures
(3 hours)
Chapter 5.2 : Considerations in Using Plant Cell Culture
The importance and advantages of plant cell culture, plant cells in
culture compare to microbes, methods used for the cultivation of
plant cells, economics of plant cell tissue culture
CHAPTER 6: BIOCONVERSION TECHNOLOGIES FOR INDUSTRIAL
BIOPROCESSES
Week 14 - 15
(7/12 – 18/12)
Week 16
(21/12 – 25/12)
Week 17 - 20
(28/12 – 15/01)
Type of bioconversion approaches e.g solid-state and liquid-state,
advantages and disadvantages of bioconversion, important factors
for bioconversion. Application of microbial, plant and animal cell
culture in bioconversion technologies utilizing waste as substrate for
production of value-added product.
Minggu Ulangkaji / Revision Weeks
Peperiksaan Akhir Semester/ Final Examination
RK 28 - Year 3
8.00-9.00
ISNIN
9.00-10.00 10.00-11.00 11.00-12.00 12.00-1.00
ERT319
Industrial
Waste
UUW322
Treatment
Thinking Skills
Taman
Muhibah Bilik Kuliah
PPKBP
1.00-2.00
2.00-3.00
3.00-4.00
Dragon - DKD 3
Dragon - Unit Operation Lab / Scale Lab
ERT316
Reaction Engineering
Dragon - DKD 4
ERT318
Unit Operations
RABU
Dragon - DKD 5
KHAMIS
ERT318
Unit
Operations
JUMAAT
ERT317
Biochemical
Engineering
Dragon DKD 2
ERT318 / ERT317
Unit Operations / Biochemical
Engineering
LAB
Dragon DKD 5
5.00-6.00
ERT318 / ERT317
Unit Operations / Biochemical
Engineering
LAB
ERT319
Industrial Waste
Treatment
SELASA
4.00-5.00
Dragon - DKD 4
EQT271
Engineering Statistics
Dragon - DKD 3
UUW235
Ethnic Relation
Dragon - DKD 5
Dragon - Unit Operation Lab / Scale Lab
ERT317
Biochemical Engineering
EQT271
Engineering Statistics
Dragon - DKD 1
Dragon - DKD 1
ERT316
Reaction
Engineering
Dragon DKD 5
6.00-7.00