ERT 317
Biochemical Engineering
Dr. Ku Syahidah(C)
Dr. Saleha Shamsudin
Course synopsis:
This course focuses on the importance of
bioprocess engineering elements such as
enzyme
technology,
fermentation
and
bioconversion
technology.
Mathematical
representations of enzymatic reaction as well as
microbial systems are featured among lecture
topics especially on kinetics study.
Learning Outcomes:
At the end of the course, students are expected to
be:
1.Ability to develop enzyme reactions based on its
kinetics study and applied catalysis.
2.Ability to evaluate microbial system based on its
metabolic pathways and kinetics study in batch
and continuous cultures.
3.Ability to propose bioconversion technologies of
microbial, plant and animal cell culture for the
production of value-added product.
List of experiments:
Lab 1: To analyze the effect of enzyme and substrate
concentration on enzyme kinetics study
Lab 2: To analyze the effect of immobilization method
on the retention of enzymatic activity
Lab 3: To analyze the effect of environmental conditions
on growth kinetics of S. cerevisae in batch process
Lab 4: To develop growth curve and effect of
immobilization on plant cell culture
Lab 5: To organize an open-ended experiment based on
bioconversion technology approaches
Learning approach:
•Lecture
: 42 hours
•Laboratory : 15 hours
•Tutorial
: 13 hours
Evaluation contribution:
•Peperiksaan / Examination
: 70 %
•Mid Term Examination 1
= 10 %
•Mid Term Examination 2
= 10 %
•Final Examination
= 50 %
•Penilaian berterusan / Continual assessment : 30 %
•Laboratories
= 15 %
Assignments / Quizzes
= 15 %
List of text books and references :
•Shuler, M. L. and Kargi, F. (2002). Bioprocess
Engineering: Basic Concept. 2nd Ed. Upper Saddle
River, NJ: Prentice Hall PTR
•Dutta, R. (2008). Fundamentals of Biochemical
Engineering. New Delhi: Ane Books India
•Katoh, S. and Yoshida, F. (2009). Biochemical
Engineering: A Textbook for Engineers Chemists and
Biologists. Weinheim: Wiley-VCH Verlag GmbH & Co
•Nielsen, J., Villadsen, J. and Gunner L. (2011).
Bioreaction Engineering Principles. 3rd Ed. New York:
Springer Science+Bussiness Media
•Najafpour, G.A. (2007). Biochemical Engineering and
Biotechnology. Amsterdam: Elsevier B.V.
Tugasan
Kandungan Kursus / Course Contents Bacaan/Re Pensyarah/Lectur
Minggu/Week
ading
(Panduan/Guidelines)
er
Assignment
CHAPTER 1: ENZYME KINETICS AND
APPLICATIONS
Chapter 1.1: Kinetics of Enzyme
Catalyzed Reactions
Week 1 - 2
(9/09 – 20/09)
Introduction
Classification
of
enzymes according to the reaction
catalyzed and how enzymes work.
Chapter 1
Enzyme kinetics - Mechanistic model
for
simple
enzyme
kinetics,
determination of rate parameters for
Michaelis-Menten kinetics; models for
more complex enzyme kinetics;
effects of pH and temperature and
insoluble substrates
Dr. Saleha
CHAPTER 1: ENZYME KINETICS AND APPLICATIONS
Chapter 1.2 : Applied Enzyme Catalysis
Week 3
(23/09 –
27/09)
Immobilized enzyme systems - Methods of
immobilization,
diffusional
limitations
in
immobilized enzyme systems, electrostatic and
Chapter 1
steric effects in immobilized enzyme systems
Enzyme production and application – Large
scale production of enzymes, medical and
industrial utilization of enzymes
CHAPTER 2: MAJOR METABOLICS PATHWAY
Introduction to metabolism and bioenergetics.
Glucose metabolism: Glycolysis and TCA cycle,
Metabolism
of
nitrogenous
Week 4 - 5 respiration.
compounds and hydrocarbons. Overview of Chapter 2
(30/9 –
biosynthesis for small and macromolecules,
11/10)
anaerobic and autotrophic metabolism.
Week 6
(14/10 18/10)
Dr. Saleha
Cuti Pertengahan Semester / Mid-semester Break
Dr. Saleha
CHAPTER 3 : KINETIC OF GROWTH IN
BATCH AND CONTINOUS CULTURES
Week 7 - 8
(21/10 – 1/11)
Chapter 3.1 : Kinetic of Growth in Batch
Culture
Quantifying cell concentrations, growth Chapter 3
patterns and kinetics in batch culture,
effects of environmental conditions on
growth kinetics, heat generation by
microbial growth.
Dr. Saleha
CHAPTER 3 : KINETIC OF GROWTH IN
BATCH AND CONTINOUS CULTURES
Week 9-10
(4/11 – 15/11)
Chapter 3.2 : Kinetic of Growth
Continuous Culture
in
Chapter 3
Some specific devices for continuous
culture, derivation for ideal chemostat,
application of chemostat and its
deviation of ideality.
CHAPTER 4: STOICHIOMETRY OF
MICROBIAL GROWTH AND PRODUCT
FORMATION
Overall growth stoichiometry and design
Week 11 (18/11 –
medium
formulation,
stoichiometric Chapter 4
22/11)
calculation of elemental balances and
degree
of
reduction,
theoretical
predictions of yield coefficients.
Dr. Ku Syahidah
Dr. Ku Syahidah
CHAPTER 5 : BIOPROCESS
CONSIDERATIONS IN USING ANIMAL
AND PLANT CELL CULTURES
Chapter 5.1 : Considerations in Using
Animal Cell Culture
Structure and biochemistry of animal
cells, methods used for the cultivation
of animal cells, products of animal cell
Week 12-13
cultures
Chapter 5 Dr. Ku Syahidah
(24/11 – 6/12)
(3 hours)
Chapter 5.2 : Considerations in Using
Plant Cell Culture
The importance and advantages of
plant cell culture, plant cells in culture
compare to microbes, methods used
for the cultivation of plant cells,
economics of plant cell tissue culture
CHAPTER 6: BIOCONVERSION
TECHNOLOGIES FOR INDUSTRIAL
BIOPROCESSES
Type of bioconversion approaches e.g
solid-state and liquid-state, advantages
Week 14 - 15 and disadvantages of bioconversion,
Chapter 6 Dr. Ku Syahidah
(9/12 – 20/12) important factors for bioconversion.
Application of microbial, plant and
animal cell culture in bioconversion
technologies utilizing waste as substrate
for production of value-added product.
Week 16
(23/12– 27/12)
Minggu Ulangkaji / Revision Weeks
Week 17 - 20
(30/12 – 24/01)
Peperiksaan Akhir Semester/ Final Examination