The University of Jordan Faculty of Agriculture Dept. of Plant Protection Prof. H. Khlaif Email: H-khlaif@ju.edu.jo Office: 227 Office hrs: All week days (10-11) Seed Pathology 606931 Time: : Mon. Wed. 14:00-15:30 Course Description : The objectives of this course is to give the students an idea about seed - borne diseases, their causal agents, including fungi, bacteria, viruses, and nematodes, their mode of transmission, economic importance, the effect on seed viability, seed anatomy in relation to pathogens transmission, the effect of environmental factors on pathogens transmission, seed healthy tests ,production of disease free seedlings, the recent techniques for the detection of these diseases in the seeds, and control methods. Week 1 2 3 4 5 6 Introduction: Historical development. Development of seed health testing, Significance Seed borne pathogens Fungi, Bacteria, Mycoplasma like organisms. Fastidious vascular bacteria, Spiroplasma, Viruses, viroids, Nematodes Location of seedborne inoculum : I Histopathology of some seedborne pathogens, Seed infection (embryo, endosperm and perisperm. seed coat and pericarp. glume infection), Seed infestation or contamination Mechanism of seed infection ( seed infection, seed infestation or contamination) Factors affecting seed infection: Host genotype, environment, crop management, type of inoculum, seed quality, plant growth stage, mother plant infection, insect infestation and pathogen interaction. Longevity of seedborne pathogens: Longevity, factors influencing longevity 7 Seed transmission and inoculation: Seed transmission ( systemic and nonsystemic), seed inoculation 8 Factors affecting seed transimission: Crop species. Environment, inoculum, survival of inoculum. cultural practices. seed abnormalities, seed germination. seed , leachates, and presence of other microflora. 9 Epidemiology and inoculum thresholds of seedborne pathogens: epidemiology ( classification of seedborne diseases based on epidemiology), inoculum thresholds, development of inoculum thresholds. 10 Nonparasitic seed disorders: Genetic effects. mechanical injuries, environmental effects. mineral deficiencies, and insect damage 11 Deterioration of seeds by storage fungi: Field and storage fungi, invasion by storage fungi. losses. conditions favoring storage fungi development, detection of damage. and control. 12 Mycotoxins and mycotoxicoses: * Mycotoxins, fungi known to produce mycotoxins, factors affecting mycotoxins production, effects of mycotoxins. and detection. 13 Control of seedboine pathogens: Introduction, selection of seed production area, crop, management, seed treatment. certification. plant quarantine, disease resistance Course outcomes: At the end of this course the students will be able to : 1. Know the different diseases transmitted by seeds. 2. The role of seeds as a source of inoculum. 3. The economic importance of seedborne diseases. 4. Recent techniques of seed testing for detection of seedborne disease 5. Production of healthy seeds. Class participation: Students should attend class on time, participate in discussion and other activities. Exams and Grades Exams Grades Medterrn.exam 30% Presentahon and term papers 30% Final Exam 40% Day Date References l -Vijendra K. Agarwal. Sinclair, J.B 1987 Principles of Seed Pathology. vol. 1. and 2. CRC Press, Inc Boca Raton, Florida. 2- S. B. Mathur, Johs Jrargensen, Technical Centre for Agriculture and Rural Cooperation (Ede, Netherlands), 1993, seed pathology