Agenda 11/17/14- Photosynthesis • Cellular Respiration Quiz • Intro to Photosynthesis worksheet • New Information: Photosynthesis and Plant Cell Specialization • Photosynthesis Diagram worksheet • HW- finish packet THE BASICS OF PHOTOSYNTHESIS • Most plants are photosynthetic autotrophs, as are some bacteria and protists – Autotrophs: generate their own organic matter through photosynthesis – Sunlight energy is transformed into energy stored in the form of chemical bonds (a) Mosses, ferns, and flowering plants (b) Kelp (c) Euglena (d) Cyanobacteria Light Energy Harvested by Photosynthetic Autotrophs 6 CO2 + 6 H2O + light energy → C6H12O6 + 6 O2 Food Chain Waxy coating Blade Vein Stem • The location and structure of chloroplasts Chloroplast LEAF CROSS SECTION MESOPHYLL CELL LEAF Mesophyll CHLOROPLAST Intermembrane space Outer membrane Granum Grana Stroma Inner membrane Stroma Thylakoid Thylakoid compartment Chloroplasts Intermembrane space Thylakoid Outer membrane Granum Inner membrane Stroma A Chloroplast Light Energy H2O CO2 Chloroplast NADP+ ADP Grana LightDependent Reactions Calvin Cycle ATP NADPH O2 Sugars Chemical Energy 2 Pathways of Photosynthesis • Light-dependent reactions – Light energy absorbed – Occurs across thylakoid membranes – Produces ATP and NADPH (and waste O2) • Light-independent reactions or Calvin Cycle – Does not require light energy – Occurs in the stroma – Produces sugar using CO2 ATP ATP - Adenosine Triphosphate Adenine Ribose 3 Phosphate groups Formation of ATP ADP ATP Energy Adenosine diphosphate (ADP) + Phosphate Partially charged battery Adenosine triphosphate (ATP) Fully charged battery ATP stores enough energy for many cellular functions. A Chloroplast Light Energy H2O CO2 Chloroplast NADP+ ADP Grana LightDependent Reactions Calvin Cycle ATP NADPH O2 Sugars Chemical Energy H2O CO2 Sunlight Energy NADP+ ADP Stroma Thylakoids ATP NADPH O2 C6H12O6 Light Dependent Reactions • Pigments arranged into photosystems – Photosystem I (happens 2nd) – Photosystem II (happens 1st) • Inputs: – H2O – Light Energy – ADP, NADP+ • Products (go on to fuel Calvin Cycle): – ATP, NADPH – O2 Light Dependent Reactions • Electron transport chain – Pumps H+ ions across the thylakoid membrane- results in energy for ATP production • ATP Synthase (enzyme) – H+ ions pass through the thylakoid membrane via ATP synthase, making it spin (energy) – ATP synthase spins, binding ADP and a phosphate group into ATP The Two Photosystems of the Light Reactions: P-680 and P-700 Potential Energy Off to the Calvin Cycle H2O CO2 Sunlight Energy NADP+ ADP Stroma Thylakoids ATP NADPH O2 C6H12O6 Light-Independent Reactions, aka Calvin Cycle • occurs in stroma • uses ATP and NADPH from the light-dependent reactions and CO2 to make the organic molecule glucose (C6H12O6) The Calvin Cycle CO2 Enters the Cycle Energy Input •Inputs: • ATP • NADPH • CO2 • Products: • C6H12O6 • ADP • NADP+ 5-Carbon Molecules Regenerated 6-Carbon Sugar Produced Sugars and other compounds H2O CO2 Sunlight Energy NADP+ ADP Stroma Thylakoids ATP NADPH O2 C6H12O6 Agenda 11/18/14- Photosynthesis and Plant Pigments • Bellwork • New Information- Plant Pigments and Properties of Light • Lab- Plant Pigments and Chromatography • HW- finish lab, formal lab report due Thurs. Properties of Light • particle nature – Photon • wave nature – photons move in waves; wavelength • when a photon strikes a substance, it may – be reflected – be absorbed – be transmitted Why Are Plants Green? Electromagnetic Spectrum and Visible Light Gamma rays X-rays UV Infrared & Microwaves Visible light Wavelength (nm) Radio waves WHY ARE PLANTS GREEN? Different wavelengths of visible light are seen by the human eye as different colors. Gamma rays X-rays UV Infrared Visible light Wavelength (nm) Microwaves Radio waves Why are plants green? The color of light seen is the color REFLECTED, NOT ABSORBED • Chloroplasts absorb light energy and convert it to chemical energy • What colors of light does chlorophyll absorb? Reflect? Light Reflected light Absorbed light Transmitted light Chloroplast Chloroplast Pigments • Thylakoids in chloroplasts contain several pigments • • • • • Chlorophyll a (bright green) Chlorophyll b (yellow-green) Carotenoids (yellow/orange) Xanthophylls (yellow) Anthocyanins (red) Figure 7.7 Why do plants have pigments other than chlorophyll? • • • • • Chlorophyll a (bright green) Chlorophyll b (yellow-green) Carotenoids (yellow/orange) Xanthophylls (yellow) Anthocyanins (red) Figure 7.7 Different pigments absorb light differently Green to Greenishyellow Yellow/Orange/R ed Absorbed Photons • energy from photon is transferred to the substance that absorbs it – electron jumps to a higher energy level – This fuels the production of ATP, NADPH • pigments absorb light energy Chlorophyll Photosynthesis Activity Site Endosymbiotic Theory (Lynn Margulis, 1981) • Mitochondria and chloroplasts may have originated as free-living prokaryotes that lived symbiotically within cells, leading to eukaryotes. • Evidence: – Circular DNA – Two or more cell membranes, with the innermost one similar to prokaryotic cell membranes – Ribosomes Agenda 11/19- Photosynthesis and Cellular Respiration • Bellwork • Finish Lab- calculate Rf values, answer lab questions, discuss lab report • Review for test Spinach Leaf Pigments Distance travelled by solvent: 65 mm Concept Map Section 8-3 Photosynthesis includes Lightdependent reactions Calvin cycle use take place in Energy from sunlight Thylakoid membranes to produce ATP Go to Section: NADPH O2 takes place in Stroma uses ATP NADPH of to produce Chloroplasts High-energy sugars Compare/Contrast Photosynthesis Cellular Respiration Energy Storing or Releasing Energy storing Energy releasing Products Glucose, oxygen gas Carbon dioxide, water, energy (ATP) Reactants Carbon dioxide, water, light energy Glucose, oxygen gas Location Chloroplast Mitochondrion Typical Leaf Cross Section Model Chapter 23, page 596 Cuticle Veins Epidermis Palisade mesophyll Xylem Vein Phloem Spongy mesophyll Epidermis Stoma Guard cells