FORENSIC CHEMISTRY
AS SCIENTIFIC AND EDUCATIONAL SPECIALISATION
AT THE JAGIELLONIAN UNIVERSITY
Paweł Kościelniak
JAGIELLONIAN UNIVERSITY, Faculty of Chemistry
INSTITUTE OF FORENSIC RESEARCH
Cracow, Poland
Jagiellonian University
(1364)
Faculty of Chemistry
1950-52
Institute of Forensic
Research
1929
DEPARTMENT OF
ANALYTICAL CHEMISTRY
(25)
GROUP OF
TOXICOLOGICAL
AND
PHARMACEUTICAL
ANALYSIS
(5)
LABORATORY
FOR
FORENSIC
CHEMISTRY
GROUP OF
CHEMOMETRICS
(3)
GROUP OF
ANALYTICAL
FLOW
TECHNIQUES
(11)
LABORATORY FOR FORENSIC CHEMISTRY
founded in 2000
- system for capillary electrophoresis
(PrinCe 550 CSD, Prince)
- HPLC chromatograph (LaChrom, Merck)
- GC chromatograph (3900, Varian)
- atomic fluorescence spectrometer with
hydride generation (AFS 230, Beijing Haiguang
Instrument Co.)
- system for microwave digestion (MARS 5X,
CEM),
- audiovisual equipment (video-projectors,
digital photo-cameras, video-camera,
video-recorders)
Sem.
No. of hours
Subject
lectures
VI
seminars
total
Electronics and automatics
30
30
Optional
60
60
90
90
Total
Electronics and automatics
VII
classes
60
60
Advanced analytical chemistry
45
Chemometrics and biometrics
30
Analysis in forensic chemistry
15
15
Radiometric analysis
30
30
Toxicology
30
30
Flow analysis
15
15
Optional
60
60
Total
225
45
15
60
15
45
300
Sem.
No. of hours
Subject
lectures
classes
total
Radiometric analysis
45
Forensic toxicology
60
15
75
60
15
90
Criminalistics
15
Low aspects of the forensic expert work
15
VIII
45
15
Student’s report
30
Optional
60
Total
100
Laboratory for master thesis
30
60
100
100
450
Seminar for master thesis
IX
i
X
seminars
315
450
60
60
Clinical toxicology
15
15
GLP
15
15
Optional
60
60
Total
90
450
60
600
LABORATORY CLASSES
Subject
No.
Place
1
Toxicological analysis of drugs
IFR
2
Ethanol- selected toxicological and analytical aspects
IFR
3
Application of GC/MS method to analysis of drugs in biological materials
IFR
4
Determination of inorganic toxicants in biological material
IFR
5
Chemical examinations of car paints
IFR
6
Chemical examinations of fibers
IFR
7
Chemical examinations of gun-shot residuals
IFR
8
Chemical examination of inks by TLC
IFR
9
Chemical analysis of inks by CE
JU
10
Determination of Se and As in biological materials by AFS-HG
JU
11
Comparative examination of psychotropic drugs by HPLC and CE
JU
12
Profiling of drugs – physicochemical and chemometric examination
JU
RESERCH SUBJECTS
Subject
Determination of selected elements at normal levels in biological
materials
Analytical method
FCh JU
IFR
ICP/OES, AAS
AAS
Development of novel techniques for preparation of biological
samples
HPLC, CE
Development of analytical procedures for determination of
psychotropic drugs
HPLC, CE
Development of analytical procedures for determination of selenium
and arsenic in biological materials
AFS
Application of Py-GCMS to analysis of criminalistic traces
Identification and discrimination of paint traces
Examination of questioned documents on the basis of identification
of chemical composition of inks
Py-GCMS
chemometric
methods
CE, LIBS
IR
PUBLICATIONS
M. Zadrożna, M. Gawlik, B. Nowak, A. Marcinek, H. Mrowiec , S. Walas, R. Wietecha-Posłuszny, P. Zagrodzki, „Antioxidants Activities and
Concentration of Selenium, Zinc and Copper in Preterm and IUGR Human Placentas”, J. Trace Elem. Med. Biol., 23, 144-148 (2009).
M. Szafarska, M. Woźniakiewicz, M. Pilch, J. Zięba-Palus, P. Kościelniak, “Computer Analysis of ATR-FTIR Spectra of Paint Samples for
Forensic Purposes”, J. Molec. Struct., 924–926, 504–513 (2009).
B. Trzcińska, J. Zięba-Palus, P. Kościelniak, "Application of Microspectrometry in Visible Range to Differentiation of Car Solid Paints for
Forensic Purposes", J. Molec. Struct., 924–926, 393–399 (2009).
R. Wietecha-Posłuszny, J. Dobrowolska, P. Kościelniak, P. Zagrodzki, „Determination of Selenium as a Biomarker of Thyroid Cancer by
HG-AFS Method”, Acta Chim. Slov., 56, 441-446 (2009).
J. M. Milczarek, M. Dziadosz, J. Zięba-Palus, “Way to Distinguish Car Paint Traces Based on Epoxy Primer Layers Analysis by Pyrolysis Gas Chromatography - Mass Spectrometry (Py-GC/MS)”, Chem. Anal., 54, 173-185 (2009.
K. Madej, „Microwave-assisted and Cloud-point Extraction in Dtermination of Drugs and Other Bioactive Compounds”, Trends in
Analytical Chemistry, 28, 436-446 (2009).
K. Madej, A. Biedroń, A. Garbacik, „Study of Separation and Extraction Conditions for Five Neuroleptic Drugs by an LLE-HPLC-DAD
Method in Human Plasma”, Journal of Liquid Chromatography & Related Technologies, 32, 3025-3037 (2009).
E. Florek, E. Ignatowicz, W. Piekoszewski, “Effect of Pregnancy and Tobacco Smoke on the Antioxidant Activity of Rutin in an Animal
Model”, Pharmacological Reports, 61, 935-940 (2009).
M. Woźniakiewicz, J. Kuczara, P. Kościelniak, „Determination of Fluoxetine in Blood Samples by High-performance Liquid
Chromatography Using a Derivatization Reagent”, Chem. Anal. (Warsaw), 54, 667-677 (2009).
R. Wietecha-Posłuszny, T. Lech, P. Kościelniak, “Application of Three Spectrometric Methods to Total Selenium Determination in Postmortem Material in a Case of Acute Selenium Compound Poisoning”, J. Forensic Sci., in print.
M. Szafarska, R. Wietecha-Posłuszny, M. Woźniakiewicz, C. Hughes, P. Kościelniak, „Influence of Storage Conditions on Ageing of Colour
Dye-Based Inkjet Printing Inks”, Problems of Forensic Sciences, in print
Ph.D. STUDY
Student
Subject
Defense
Renata Wietecha-Posłuszny
Development of the analytical procedure for the selenium determination in
human tissues by the AFS-HG technique
2004
Rafał Borusiewicz
Examination of arson traces
2004
Joanna Mania
Examination of inks by capillary electrophoresis
2005
Michał Woźniakiewicz
Analysis of psychotropic drugs by HPLC and CE
2008
Justyna Dobrowolska
Elemental analysis of biological materials
2008
Jakub Mielczarek
Classification and discrimination of car paint traces
2008
Justyna Dobrowolska
Elemental analysis of selected human and animal tissues with toxicological
and clinical respects
2009
Małgorzata Szafarska
Development of the method for discrimination of printer inks by capillary
electrophoresis for criminalistic purposes
2010
Jagoda Majcherczyk
Influence of tobacco smoke on pharmacokinetics of citalopram
2011
Aneta Garbacik
Novel methods for preparation of biological samples to toxicological analysis
2011
Danuta Dudek-Adamska
Trace elements in biological materials
2011
Agnieszka Biedroń
Analytical and toxicological examination of selected drugs in biological
materials
2012
Paweł Knihnicki
Determination of psychotropic drugs in saliva by capillary electroforesis
2012
Tomasz Lachowicz
Py-GCMS analysis as a tool in forensic examination of criminalistic traces
2012
Agnieszka Solarz
Analysis of printer inks by capillary electrophoresis for criminalistic purposes
2013
PROJECTS
TEMPUS program, project „Transformation of University Chemistry Studies at the Jagiellonian University”
(TRUCS); 1997-2000
TEMPUS program, project „National Training Program for Forensic Science Experts” (NATFOSE); 1998-2001
LEONARDO DA VINCI program, project „Vocational Training System in Forensic Sciences - Exchange of
Educational Experiences” (VITSFOS-E); 2001-2003
NATO program, project “Sensors for Chemical Weapons Detection Based on Molecularly Imprinted Polymers”;
2004-2005
TEMPUS program, project „Education System in Forensic Sciences for the Republic of Macedonia”,
(EDUFORMAK); 2007-2010
Government Grant, project „Application of the Capillary Electrophoresis as a Tool in Criminalistic Examination of
Ink Printed Documents”; 2007-2010
Operational Programme 'Innovative Economy', project „Mikro and Nano-systems in Chemistry and Clinical
Diagnostics” (MNS-DIAG); 2009-2013
Government Grant, project „The Use of Modern Analytical Instrumentation for Purpose of the Fight Against
Crime”; 2010-2013
Laser-induced breakdown spectrometer
(LIBS)
PULSED LASER
(1064 nm)
PLASMA EMMISION
PLASMA
PLUME
SAMPLE
Laser-induced breakdown spectroscopy (LIBS) is a type of atomic emission spectroscopy, which uses a
highly energetic laser pulse as the excitation source. The laser is focused to form a plasma, which
atomizes and excites samples.
Price: ~ 45 000 EUR