Mrs. Goux’s Living Environment 1 Chapter 1 Notes The Science of Biology Section 1.1 What is Science - The goal of science is to investigate and understand the natural word, to _____________________________ ___________________, and to use those explanations to make useful predictions. Section 1.2 Scientific Method - Scientists must be keen observers. - Scientific Inquiry. What is it? - ________________________________________ Observation -using one or more of the _______________________ to collect information (data). Inference - ________________________________ based experience or prior knowledge Data - information gathered from observations using our senses, usually in the form of a ____________________ Hypothesis -the process of developing a __________________________ to a problem or question. Ex. Based on observations of maggots coming from meat - living things come from nonliving matter – called __________________________________. Scientific Method – steps used to __________________________________ questions Controlled Experiment - Series of test or trials used to test a hypothesis Mrs. Goux’s Living Environment 1 - A controlled experiment always has two groups: 1) Experimental group – this is the group that __________________________________ 2) Control Group – this is the group that ________________________________ We need both groups so that we can compare them. If we only had an experimental group we would not really know if our treatment had an effect. What do scientists do? Steps of the Scientific Method 1. Ask Questions2. Make Observations3. Make Inferences4. Experiment5. Collect Data – 6. Draw Conclusions7. Repeat8. Peer Review- Experimentation - controlled experiment is used to test a hypothesis. - A good controlled experiment must have 1) Control Group – used as a ________________________________ . 2) Experimental Group – differs from the control by one variable (the group that ___________________________ or factor being tested. 3) _____________________________ is tested . All other variable are controlled. Mrs. Goux’s Living Environment 1 4) A sufficient number of test subjects. The __________________________ the results. 5) Publish the results. Variables There are ________ types of variable in a controlled experiment Dependent Independent Controlled Independent Variable – the ___________________________ – you manipulate or change this variable ( amt of water, fertilizer, temperature or time at which you collect or measure the data) Dependent Variable – what ___________________________ in the experiment. Ex. 1 Francesco Redi experimented to disprove spontaneous generation. Mrs. Goux’s Living Environment 1 Ex. 2 Louis Pasteur experimented to disprove spontaneous generation. Collecting/Analyzing Data – collecting data to see if it ______________________________ hypothesis. Drawing Conclusions A) if data _____________ hypothesis ______________________ B) if data __________________ hypothesis ____________________ Ex. The work of Redi and Pasteur allowed for the theory of spontaneous generation ______ _____________________. A new theory called biogenesis took its place. Biogenesis - states that living things __________________________________________ Scientific Theories vs. Laws or principles Theory – is a _______________________ by a body of evidence but ____________________ to be 100% true. Mrs. Goux’s Living Environment 1 Laws/principles – scientific facts that are __________________________. Organizing data often involves the use of ________________________. Graphs allow scientists to analyze _________________ data to draw conclusions. The ___________________________ is on the x-axis. The ____________________________ is on the y-axis. Examine the graph below: What is the Independent Variable in this experiment? ________________ What is the Dependent Variable in this experiment? ________________ What can be concluded from this graph? _________________________________________________ _______________________________________________ Graphing How to do a line graph 1. First pretend you are the experimenter. 2. Remember that the scale on a axis is like a ruler and that all spaces must be equal to one another on the same axis. 3. Look at the Independent Variable (I choose) and look to see the largest number. Here, it is the days I am counting the population of each species. The largest number is 5. I want to make the scale as large as possible, so it is easier to mark my data points. I do that by counting how many lines there are on the xaxis. On this graph there are 22. I round down to 20. If I divide 20 lines by the 5 days ( 20/5=4) I get 4. That means that every 4 boxes on the x-axis is equal to 1 day. So I start with o and the 4th line is 1, the 8th line is 2, 12th 4. Next you will put the Dependant or Responding Variable ( it depends on what I chose to do on the Independent variable. The population count changes depending on which day I chose to count them.). Here it is the population number of species A and B. Again my largest population number is 60 and I have 22 spaces available. Counting each space as 5 allows me to spread the scale for Populations as large as possible and still making counting simple. I chose to mark every other box . Just make sure you mark every line initially with pencil and then go back and erase every other one if you wish. Just make sure that all boxes are of equal value, like a ruler. 5. If there are more than two sets of data points on the same co-ordinates as there are here on days 1, 2 and 3 you put the dot there and mark with the circle surrounded by the triangle . Mrs. Goux’s Living Environment 1 80 60 50 40 30 20 T I on Population 70 10 0 0 1 2 3 Day 4 5 Mrs. Goux’s Living Environment 1 Section 1.3 Studying Living Things Characteristics of Living Things (must have all of these traits in order to be considered to be alive) 1) Organization – composed of ____________ Unicellular/multicellular (Levels of structure) organism 2) Reproduction – important for survival of species -which is a group of organisms that ___________________________________ - can be sexual or asexual ( 2 parents or 1 parent) 3) Universal Genetic Code – all organisms ______________ (deoxyribonucleic acid) that govern inheritance passed from parent to offspring. 4) Growth and Development growth is the __________________ or the addition of new structures Development is any change from conception to death 5) Use Materials and Energy Production Organisms need __________________________________ Chemical reactions of life are ________________________ Use food to create energy molecules called ATP Two types of organisms on Earth Mrs. Goux’s Living Environment 1 1. Autotrophs – ____________________________________ 2. Heterotrophs – __________________________________ for food 6) Response to Stimuli – responding to your environment - Appropriate responses are ___________________________ 7) Maintaining an Internal Balance - _____________________ - ex) body temperature in warm blooded organisms. 8) Adaptation - is any ______________________________________________________________________ - usually inherited from previous generations - the ______________________________________in a population over time is ______________ Organism - a living thing Organic - substance from _______________________ organism Inorganic - _________________ - Most carbon compounds with the exception of carbon dioxide are organic. -Water is the most common inorganic compound in living things. It composes 80-95% of living organisms. - Scientists have been unable to agree on a single definition that characterizes life. Mrs. Goux’s Living Environment 1 Life Processes: (all organisms including humans must do all of these to remain alive) 1. Nutrition- the ____________________________________________________by an organism for its use (a.) ingestion- ___________________________________________ (b.) digestion- ________________________________________ to simpler foods by an organism 2. Circulation - __________________________________within an organism 3. Movement- change in position by a living thing (avoids predation, allows feeding or movement towards suitable conditions 4. Respiration- process used by organisms _________________________________________________ (ex. oxygen + glucose yields carbon dioxide + water + ENERGY) ** The energy released in respiration is needed to maintain life functions. 5. Synthesis- ________________________ chemical substances to _________________ ones (ex. starches from simple sugars) “creating” 6. Excretion- the _______________________ of cellular waste products ( ex. sweating, exhaling, urinating, NOT pooping!) 7. Regulation (coordination) - the control of the various activities of an organism (mostly involves the nervous system and endocrine glands in complex organisms) 8. growth- increase in living matter by ______________________________________________________ 9. reproduction- ability of living things to make more of their own kind ** Not needed for organism survival--but necessary for the survival of the species. 10. immunity -- the ability of an organism to resist ___________________ causing organisms and foreign invaders Metabolism - the ______________________________________________ needed to sustain life (the total of all of the chemical reactions that occur in all the cells of an organism) Mrs. Goux’s Living Environment 1 Homeostasis - the __________________________________________________________________ (steady state) - A disruption in any organ system will result in a corresponding disruption of homeostasis. -Organisms possess a control mechanisms that detect changes in the internal (or external) environment and make adjustments to correct the situation. [ Examples of Maintaining Homeostasis in Living Things] 1.) environmental temp increases ----> more sweating ---> more evaporation --> cools down body 2.) Blood sugar regulation __________ -- a ______________________________ produced by the pancreas and carried through the blood -- ____________________________ 2 major ways: a.) Takes ________________________ to be used as fuel in cell respiration from our blood b.) ________________________________________ (animal starch) and ___________ it in our ___________ and ___________________________ glucogon -- another ___________________ which can ______________________ ________ our blood sugar level if it is too low The failure to maintain blood glucose homeostasis results in diabetes. 3.) Plant leaf regulation of water balance inside them. ________ -- microscopic holes in a leaf which allow for ____________________ _____________out of the plant ________ -- ________________ the stomata (pores) Changes in the stomata controlled by guard cells that _________________ _____ Mrs. Goux’s Living Environment 1 ____________________ by many plants. Section 1.4 Tools and Procedures SCIENTIFIC MEASUREMENTS SI units (International System of Units) of the Metric System Measurement Length (distance) Mass (amount of matter) Volume (amount of space matter occupies) Temperature (avg. KE of matter) SI prefixes Megakilohectodeca- Meaning 1x106 (= 106) Unit meter (m) kilogram (kg) liter (L) Celsius (°C) Ex.’s using meter Mm km Hm Dm BASE m decicenti- dm cm millimicro- mm ***m*** BASE UNIT Kilo Hecto Deca meter Ex.’s 35 cm = _________ m distance Deci Liter volume Centi Milli 642 km = ______ m 1000 m = ______ mm Mrs. Goux’s Living Environment 1 Tools for Observation & Measurement A) Light Microscope – uses ______________ to produce an enlarged view 1. Simple Microscope – ___________ lens; Ex. Magnifying glass 2. ***Compound Microscope – __uses _____ lenses: Ocular (eyepiece) – magnifies 10X Objective – usually 3-4 choices (4X, 10X, 40/43X, 100X) Total Magnification = ____________ x _____________ 3. Phase-Contrast Microscope – _____________ 4. Stereomicroscope – ____image of specimen (not reversed) studying ______ & structural features --- Used for B) Electron Microscope Transmission EM – 250,000X Uses electron beams (not light) & electromagnetic lenses (not glass); Scanning EM (SEM) LAB TOOLS & TECHNIQUES 1. Centrifugation – separates materials of different _________ 2. Gel Electrophoresis – separation based on _____________ ***_________________ molecules travel faster & farther 3. Chromatography – separating a color into their component colors based on size 4. Computers – Ex. MRI, CAT scans Generate images of body tissues 5. Staining & Indicators Stains – make cell structures more visible --- Ex. Iodine, methylene blue Indicators – substances that change _________ when they come in contact with certain chemicals --- Ex. _______ _ paper – acidic, basic or neutral Mrs. Goux’s Living Environment 1 CLASSIFICATION of LIVING THINGS Taxonomy = _______________ of organisms Makes studying the unity & diversity of living things EASIER Classification Categories From broad groupings Narrow & specific groupings (GENERAL) (SPECIFIC) Kingdom Phylum Class Order Family Genus species Every species belongs to ONE of each groupings!!! Dichotomous Key – “di” = _____; Ex. bicycle, car, jet & motorcycle 1a 1b Requires petroleum fuel Requires only muscle power go to Step 2 ____________ 2a 2b Has wings and flies Has no wings and does not fly ____________ go to Step 3 3a 3b Has two wheels Has more than two wheels __ __________ _____________ Mrs. Goux’s Living Environment 1 THE 6 KINGDOMS PROKAYOTES Prokaryotes – lack ________ membranes & most _________ (= internal membrane-bound structures); without nucleus Eukaryotes – contain _______ membranes & organelles 1.) ARCHAEBACTERIA- extreme ancient bacteria that live in environments such as high temperature above boiling, acidic environment (low pH) or highly saline(salt) environment 2.) EUBACTERIA = regular bacteria & blue-green algae --- Cell walls, ________________ (one cell) Bacteria – _________________ (cannot make own food) Blue-green algae – __________ (can make own food) RYOTES 3) PROTISTA – mostly unicellular a) Protozoa – ___________________-like movement & nutrition Ex. Amoeba & paramecium b) Algae – can form colonies, do photosynthesis, have cell walls, ___________nutrition Ex. Spirogyra, kelp, diatoms, seaweed 4.) FUNGI – mostly ______________ (many cells) --- __________________ on other living things Heterotrophs _______ of other organisms Mrs. Goux’s Living Environment 1 Cell wall (made of ____________________) Ex. Yeasts, molds, mushrooms 5.) PLANTAE (Plants) – mostly multicellular Autotrophs = photosynthetic organisms (can make own food using energy from ______________________) Cell wall (made of _________________) Ex. Roses, oak & maple trees, cactus, dandelions 6.) ANIMALIA (Animals) – multicellular Heterotrophs ___________________ reproduction is very common a) Invertebrates – sponges, worms, insects Have NO ___________________ b) Vertebrates – fish, amphibians, reptiles, mammals, birds Key Organisms (in order of increasing complexity): Amoeba – has _________________ (false feet) for food absorption & movement Paramecium – has __________ for movement & an ______________ (side pocket) through which food is ingested Hydra --- __ cell layers thick --- Has a mouth surrounded by ___________ leading to an internal cavity Earthworm --- _______________________ circulation and tube-like digestion Grasshopper --- ____________________ circulation and tube-like digestion Humans --- Mammals nourish young with ___________________, have hair/fur & have well-developed brains Mrs. Goux’s Living Environment 1