Anatomy & Physiology of the Pig ANSC 4401 Swine Production Why understand pig A&P? It will open new areas of application of pig biology to benefit humans It helps you have a conversation with other pig people, veterinarians and scientists It will help you manage pigs in meaningful ways on farms Recognize disease states more easily Obtain blood or other tissue samples Anatomy & Physiology Outline Anatomical terms Major bones Uses for pigs other than for eating Some terminology Blood collection Necropsy Anatomical terms Major bones of the pig Uses of pigs other than for eating Organ donors As a source of biological materials, ex. Insulin or heparin As a model for biomedical research As an organ donor to humans For entertainment As pets As truffle-finders (they are smell experts) Xenotransplantation & Cloning New pig cloning research promising yet risky, studies indicate August 17, 2000 Web posted at: 12:57 PM EDT (1657 GMT) WASHINGTON (CNN) -- Two groups of researchers publishing work in rival scientific journals report similar successes in cloning pigs -- a difficult process that may lead to ways to grow organs in the animals destined for human transplantation. "The goal here is to reduce the shortage of organs for transplantation and prevent any more needless deaths that arise because people simply don't receive the organ they need," said Dr. Anthony Perry of New York's Rockefeller University. Some Terms Serum (fluid minus all cells and clotting factors) Plasma (fluid including soluble clotting factors) Red blood cells (obtained only from plasma) White blood cells (obtained only from plasma) Composition of Newborn Pigs Table 1. Composition of newborns of three species and neonatal pigs. Data are percentage of total body weight. Species Water Protein Fat Human, newborn 69.1 11.9 16.1 Rat, newborn 86.0 10.8 1.1 Pig, newborn 84.1 11.3 1.1 Pig, 7 days old 68.5 13.6 9.5 Pig, 28 days old 61.8 14.4 17.8 From Widdowson, 1950 and Manners and McCrea, 1963. Blood Collection From Pigs Ear veins Jugular vein Facial vein Tail vein Orbital Sinus Points to sample from or inject into the venous system of pigs Piglets 1. Ventral Neck Vena Cava External Jugular Lingual-facial Facial Young pigs difficult difficult Adults 2. Orbital sinus 3. Ear vein difficult difficult 4. Tail vein difficult difficult not advised 5. Heart (cardiac puncture) na* na* na* * not advised except as a last resort or in the case of euthanasia Preferred method of restraint on back on back standing, snared Common Bleeding Ports Orbital venous sinus Facial Vein Auricular (ear) vein External Jugular Vein Bleeding Trough for Young Pigs Bleeding tools: Vacutainer Bleeding sows Blood Collection Tubes Table 2. Common blood collection tubes (tubes with * are commonly used) Color of tube top Fluid type Anticoagulant Example uses Red* Serum none Antibodies, minerals, other proteins Blue Either Na heparin or none Special blood chemistries Brown Plasma Na heparin Lead determinations & others Black/light blue Plasma Na citrate Coagulation studies Gray Plasma glycolytic inhibitors Glucose determinations Green* Plasma lithium heparin Na, Ca sensitive assays Yellow Plasma sodium citrate DNA extraction Purple/Lavender* Plasma EDTA Clotting factors Maximum safe blood draw Total blood volume, mL Maximum draw, mL Newborn, 3 lb 110 10 Nursery pig, 35 lb 1,280 120 Sow, 440 lb 16,000 1,600 Age & weight Selected normal blood values Measure Blood volume WBC count Neutrophils Lymphocytes Gamma globulin Value 8% 10-12 thousand/μL 45 % 50 % 25-30 mg/mL Necropsy – Why? To improve the herd health To contribute to the herd health program To identify causes of illness or death so that Effective treatments can be applied Preventative measures can be implemented Necropsy – What to look for Skin condition Swollen joints Wounds or abscesses Intestinal condition (fluid-filled, bloody, gas, etc) Lung condition (hemorrhage, non-functional tissues, edema, etc.) Liver condition (look for milk spots; indication of a parasitic infection) Condition of other organs (kidneys, spleen, heart) Snout condition (condition of the turbinates) Necropsy – “normal” Necropsy -- Tools Scalpel Probes (spatula or equivalent) Saw (hack saw or equivalent) Water Mechanism to dispose of parts (plastic bags) The End