Genikon
The New 3D Generation of in Situ Fluorescence Hybridisation
The Motorization of the 90i Nikon Microscope
Nikon Software interface for the control of the microscope
Dapi
+
Fitc
+
Tritc
=
Epi_fluorescence turret motorization
Fish Analysis on lymphocyte, chromosome 4
(rearranged photo b (Dr.Antonio Novelli, Roma)
The new digital head of the
microscope 90i/80i gives the
opportunity to be controlled from
software in the moving of the single
filter in a fast a very easy way. This
opportunity eliminates shifting and
bleaching problems. The software
offers list of capturing saved and
the complete control of the shutter
of the fluorescence in order to
illuminate as less as possible the
sample.
The evolution of the microscopes during last years ad their Automation
(z axe control, control of filter set ) gave a big contribution to the
precision of the acquisition of images in FISH technique. The fusion of
Nikon microscopes with Genikon “Digital System” gives today the ideal
solution to acquire and to process FISH images in a very quick and
simple way. In particular, the automatic control of the times acquisition
of the cameras, gives possible a completely automatic solution.
Building of the fluorochromes list by the set up
of the number of planes of focus
Microscope’s filters control by Genikon interface
With just a click of the mouse the user starts the
acquisition process of all the fluorochromes of the list
and gives to the system the complete control of the
exposition times in order to obtain at the end a perfect
and focussed image.
Autofocus control Tool
Senza
“Without Autofocus tool”
“With Autofocus tool”
The new tool of automatic focus control, identifies the best
contrast for every plane for each fluorochrome independently
from the one fixed at the ocular
Set up of Acquisition sequence on every plane of focus
Fusion Image of every
focus planes
Single plane image
Because in a single plane of fluorescence could
happen that one signal doesn’t show the same focus
of the other, the new tool of 3D reconstruction of
Genikon became very important. This function will
create one single image which will show all the single
signals (for the same fluorochrome) at the same
focus plane.
Thanks to the new
techniques of 3 D
reconstruction and of
focus control, it’s
possible to execute a
building of Karyotyping
also on Fish samples
Karyotyping on Fish images
Funzione “Dapi reverse”
The DAPI reverse
function it’s showing the
probe and helps the user
to recognise
chromosomes
Genikon with the 3D for
automatic method of counting
spot, offers a new procedure
fast and precise to obtain the
value of the ratio of spots on
single nuclei or many nuclei
identified from the user on
HER2NEU samples.
HER2/Neu Analysis
NIKON INSTRUMENTS S.p.A.
Firenze (Calenzano) Via Meucci N.59
Tel.055 3009601 - Fax 055 300995
www.nikon.it e-mail:info@nikon.it