Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) ENERGY Remember the cell theory: “energy flows through living things during cellular metabolism” Autotrophs: plants, some protists and some _____________________ are able to use the energy from the sun to produce food. Heterotrophs: animals, some prokaryotes, some _____________________ cannot use the sun’s energy directly, and need to consume ________________________ as a food source. Fungi are also __________________________ that _________________________ their food in order to obtain the nutrients. Yeast are _____________________________ fungi. ATP ATP stands for ___________________________________________________________________________, because it contains 3 phosphates. ATP is a nucleic acid that plants and animals use for __________________. It carries energy in its chemical bonds. Energy is released when the bonds are ______________________. ADP (adenosine di-phosphate) has only ______________ phosphates and has ____________ energy than ATP. CELLS USE ENERGY (Review) Active transport: moves ions across cell membrane through protein channels and pumps Sodium-Potassium Pump – moves ions, 3 Na+ out and 2 K+ ions into the cell Synthesis of Nucleic Acids: DNA, RNA, ATP Reproduction: Mitosis and meiosis Movement: work and transport across the cells using ATP o ATP is good for _______________term use, it is poor at long-term energy storage. Glucose and lipids are better for __________ term energy storage. Glucose stores 90X more energy than ATP. Cells keep a small supply of ATP; they generate more from ADP when needed, by breaking down carbohydrates like glucose. SCIENTISTS WHO DISCOVERED PHOTOSYNTHESIS Van Helmont (1643): Discovered that most of the __________________ of a plant comes from water. Joseph Priestly (1771): Discovered plants produce a substance that kept a candle burning. Plants release __. Jan Ingenhousz (1779): Discovered that plants only produce oxygen in the presence of __________________. Melvin Calvin (1948): Determined the details of the Calvin Cycle. Photosynthesis using light (photons) to make glucose (C6H12O6) - (ie. Transforms light E into chemical E) Photosynthesis takes place in ________________________ in plant cells and the ___________ ________________ in prokaryotes. It uses a pigment that _________________ high energy red and blue light called ___________________. This pigment appears green since it reflects green light. There are several pigments in plant cells. Chloroplasts have and _________________ and ______________ membrane. The stroma is the space and the granum are stacks of thylakoids that contain the chlorophyll. In photosynthesis, the LIGHT DEPENDANT REACTION takes place in the ________________ and the LIGHT INDEPENDENT REACTION takes place in the ____________________. Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) The overall reaction of photosynthesis occurs in 2 reactions in the chloroplast: LIGHT DEPENDENT REACTION and the LIGHT INDEPENDENT REACTION (CALVIN CYCLE) Overall equation of photosynthesis: Carbon dioxide + water produces -> glucose + oxygen = 6CO2 + 6H2O -> C6H12O6 + 6O2 LIGHT REACTION (photophosphorylation): takes place in the thylakoids and is composed of PHOTOSYSTEM I and PHOTOSYSTEM II. Uses light and water to make the energy storing molecules _________________ and __________________ (which are used in the Calvin cycle), and releases oxygen as waste. 1. A photon of light is absorbed by chlorphyll in PHOTOSYSTEM II in the thylakoid membrane. 2. PHOTOSYSTEM II: the energy from the photons of light are transferred to electrons, which are carried to the electron transport chain (ETC) 3. Electrons in PHOTOSYSTEM II split a molecule of H2O into H+ (makes ATP and NADPH) and O2 (waste). 4. Electrons from PHOTSYSTEM II are moved to ETC to provide energy for the synthesis of ATP. ATP Synthase, a specialized ________________ channel in the thylakoid membrane at the end of the ETC converts ADP to ATP as H+ ions pump through to the outside of the membrane. ATP synthase turns/spins like a turbine as ions pass through. Chemiosmosis: occurs as H+ ions move across the membrane to make ATP. (like osmosis but with chemical ions instead of water). 5. A photon of light is absorbed by chlorophyll in PHOTSYSTEM I and combines with electrons from ETC. Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) 6. Electrons and H+ (from PSII) convert NADP+ + H+ __________ (nicotinamide adenine dinucleotide phosphate) an energy electron carrier. 7. ATP and NADPH are the products of the LIGHT REACTION and are carried to the stroma to the Calvin Cycle (LIGHT INDEPENDENT REACTION. Oxygen is also produced as a waste byproduct that is used in cellular respiration and released by the plant as a gas. CALVIN CYCLE (LIGHT INDEPENDENT REACTION) – does not need sunlight. Uses ATP + NADPH to convert CO2 into GLUCOSE, takes place in the stroma. Cycles 6 times to make glucose. 6 molecules of CO2 enter the cycle from the air. They fixed by the enzyme ___________________, (which is the most abundant protein in the world) to combine with RuBP (5C) and make 3Phosphoglycerate (3C). ATP and NADPH are used to convert the 3C molecules into higher-energy forms (endergonic) It takes 2 3-carbon molecules to form a 6-carbon sugar such as glucose. The rest of the carbons are resued to make RuBP so the cycle continues. Overall, the Calvin Cycle uses ______ molecules of CO2 to make _____molecules of glucose and cycles ____ times. Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) Reaction Formulas: Light Dependent Reactions in the _____________________ and use _______________ to capture photons of light. NADP + H excited e- _________________ + _____________ ADP + P ATP synthase _______________ Light Independent Reaction (Calvin Cycle) ATP + NADPH + CO2 ______________________ Oxidation-Reduction Reactions always take place together: Oxidation: lose e- and H+, gain O2, release energy. Reduction: lose O2, gain e- and H+, takes in energy. What factors can effect photosynthesis? Light intensity: more will ______________________photosynthesis Carbon Dioxide amount: more will _________________photosynthesis Temperature: too high or too low will ___________________ photsynthesis Plants have adapted to close stomata during the day (CAM or desert plants); deciduous plants lose their leaves in the fall and do not perform photosynthesis in the winter; etc. Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) Cellular Respiration converts chemical energy into usable energy for the cell. All organisms use cellular respiration in one form or another to use energy. Chemical Energy: We need to consume food so we can break it down into _______________energy, like glucose The amount of food we can get from food is calculated by the amount of ________________ it contains. Calorie = amount of energy needed to raise the temperature of 1 g of H2O by 1 degree Celsius. You do not use all the glucose in food at once: your cells _______________ release the energy it contains over time. This is measured by your ________________________. Mitochondria: The site of cellular respiration in eukaryotes. Structure is made of ______membranes (inner and outer); the folds called the ______________ and the space called the _________________. Cellular Respiration: To release energy by breaking down food molecules in the presence of ___________________. Formula: C6H12O6 = 6H2O + energy (heat and ATP) + 6CO2 It is an exergonic reaction There are 3 steps to cellular respiration: Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) Step 1: Glycolysis Occurs in the ________________________ outside of the mitochondria. The process of breaking 1 molecule of glucose into 2 molecules of __________________________. Glycolysis is fast and this step of respiration does ______________ require oxygen (______________________). Because glycolysis is so fast, the cell uses up its available NAD+ quickly, and then ATP production will stop unless more is made (NAD+ is regenerated in the Electron Transport Chain in the mitochondria) Produces ____ ATP molecules and ______ NADH molecules which are transported to the ETC. Step 2: Krebs Cycle or Citric Acid Cycle. The ____________________made during glycolysis is converted into _________________ which enters the Citric Acid Cycle and used to make CO2, NADH, ATP and FADH2 inside the matrix of the mitochondria. The Krebs cycle turns 2 times for each molecule of glucose and makes ___molecules of CO2, ___molecules of NADH, __molecules of ATP and ___ of FADH2. Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) Step 3: Electron Transport Chain and Chemiosmosis The ETC uses high energy electrons from Glycolysis (NADH) and the Krebs Cycle (NADH and FADH2) to convert ADP to __________. The ETC is located in the inner membrane of the mitochondria and requires ________________. (for prokaryotes the ETC is in __________________) The ETC oxidizes NADH to _______________ and FADH2 to ____________. As a result high energy e- are pumped through the inner membrane and H+ are released into the intermembrane space. The high energy e- combine H+ + O2 to produce ________________ (the final electron acceptor). The H+ ions are pumped back through the membrane by ATP ___________________ to make ________ ATP. Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9) Fermentation: When oxygen is __________ present, glycolysis is followed by fermentation, an _______________ process. The purpose is to replenish NAD+ for glycolysis (since it can not be made in the ETC). There are 2 types: Lactic Acid Fermentation: occurs in muscle cells when not enough oxygen is supplied Pyruvic acid + NADH lactic acid + NAD+ Alcoholic Fermentation: occurs in yeast and some bacteria (make bread, alcohol, etc) Pyruvic acid +NADH ethyl alcohol + CO2 + NAD + *** Cellular Respiration results in the production of 36 ATP molecules Process ATP release Where in the cell? Need O2 Type of Respiration Glycolysis Krebs or Citric Acid Cycle Electron Transport Chain Fermentation Total 36 Overview of Photosynthesis and Cellular Respiration PHOTOSYNTHESIS CELLULAR RESPIRATION FUNCTION Energy storage Energy release LOCATION Chloroplasts mitochondria REACTANTS CO2 and H20 C6H12O6 and O2 PRODUCTS C6H12O6 and O2 CO2 and H20 EQUATION 6CO2 + 6H2O C6H12O6 + 6O2 C6H12O6 + 6O2 --> 6CO2 + 6H2O Unit 5 Photosynthesis and Cellular Respiration (Chapters 8 and 9)