Biology/Chemistry Interface 2
“Pump House Gang”
Carmen Domingo
Christian Hansel
Jennifer Breckler
Kimberly Tanner
Melina Hale
Tom McKnight
Wayne Versaw
Coaches - Stacy Hagemeier and Mark Hens
Teachable Unit: Membrane Transport
Overall goal:
Students will understand how cells exchange
substances with their environment.
Setting:
Introductory Biology class for majors
~150 students
Overall goal: Students will understand how cells
exchange substances with their environment.
Specific student learning outcomes for the unit:
1) Be able to explain the basis of selective membrane permeability
and distinguish between passive and active mechanisms of
membrane transport.
2) Be able to distinguish structural characteristics and functional
roles of the various membrane transport proteins (i.e. carriers,
channels, pumps)
3) Describe the physiological importance of membrane transport (i.e.
energy considerations)
4) Predict the outcomes of disruption of various membrane transport
systems
Relationship of our Tidbit to Learning Outcomes:
1) Be able to explain the basis of selective membrane
permeability and distinguish between passive and active
mechanisms of membrane transport.
2) Be able to distinguish structural characteristics and functional
roles of the various membrane transport proteins (i.e. carriers,
channels, pumps)
3) Describe the physiological importance of membrane transport
4) Predict the outcomes of disruption of various membrane
transport systems
Rationale for a teachable tidbit
When it comes to secondary active transport, many
students have difficulty understanding the source of
energy needed to move molecules against their
concentration gradient
Bio 101
Today….
Congratulations!
Exam review
Exam 2, Question #4
What happens to glucose when the Na/K pump is blocked?
A. No effect on glucose transport
B. Glucose transport into the cell stops immediately
C. Glucose flows out of the cell
D. Glucose transport into the cell stops but not immediately
Exam 2, Question #4
What happens to glucose when the Na/K pump is blocked?
A. No effect on glucose transport
B. Glucose transport into the cell stops immediately
C. Glucose flows out of the cell
D. Glucose transport into the cell stops but not immediately
40
35
30
25
Percent
20
Q #4
15
10
5
0
A
B
C
D
Review: active transport of glucose into the cell
http://www.stolaf.edu/people/giannini/flashanimat/transport/secondary%20active%20transport.swf
What happens to glucose when the Na/K pump is blocked?
Role Play
A tragedy in three acts
BACK (outside cell)
G
PROLOGUE
G
Aisle
(Na+/K+
Pump)
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+ Na+ Na+
Na+
Na+
Na+ Na+
Na+
Na+
Na+
Tables
(membrane) Aisle
Na+
Na+
Na+
Na+
G
G
G
G
G
G
G
G
G
G
(Glucose
Trans)
Na+
Na+
G
G
G
G
G
Na+
FRONT (inside cell)
G
(HUGE amounts of
Glucose)
BACK (outside cell)
Na+
Na+
Na+
Na+
Na+
Na+
Aisle
(Na+/K+
Pump)
ACT 1
Build gradient
ATP
QuickTime™ and a
decompressor
are needed to see this picture.
Na+
Na+
G
G
Tables
(membrane) Aisle
ADP
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
G
G
G
G
G
G
G
G
G
Na+
Na+
Na+
G
G
(Glucose
Trans)
Na+
Na+ Na+ Na+
G
G
G
G
G
FRONT (inside cell)
(HUGE amounts of
Glucose)
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
BACK (outside cell)
Na+
Na+ Na+
Na+
Na+
Na+
Na+
Aisle
(Na+/K+
Pump)
END ACT 1
Gradient
established
G
Na+
G
G
G
Tables
(membrane) Aisle
G
G
G
G
G
G
G
G
G
G
G
G
G
(Glucose
Trans)
Na+
ATP
QuickTime™ and a
decompressor
are needed to see this picture.
ADP
Na+
G
(HUGE amounts of
Glucose)
FRONT (inside cell)
BACK (outside cell)
Na+
Na+ Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
Aisle
(Na+/K+
Pump)
ACT 2
glucose
transport
Na+
Na+
Na+
Na+ Na+
ATP
G
Na+
Na+
G
G
G
G
G
G
G
G
(Glucose
Trans)
ADP
Na+
G
Tables
(membrane) Aisle
G
QuickTime™ and a
decompressor
are needed to see this picture.
G
G
Na+
G
Na+
G
Na+
G
Na+
G
G
Na+
FRONT (inside cell)
(HUGE amounts of
Glucose)
Na+
BACK (outside cell)
Na+
Na+ Na+
Na+
Na+
Na+
Na+
Na+
Na+
Na+
ACT 3 tragic
inhibition
Aisle
(Na+/K+
Pump)
Na+
Na+
Na+ Na+
Na+
G
G
G
G
G
G
G
G
(Glucose
Trans)
G
Na+
Na+
G
Tables
(membrane) Aisle
G
Na+
G
G
Na+
G
Na+
G
Na+
G
Na+
G
G
Na+
FRONT (inside cell)
(HUGE amounts of
Glucose)
Na+
BACK (outside cell)
Na+
Na+
Na+
Na+
Na+
Na+
Na+
G
Na+
Na+
G
Na+
Na+
G
Tables
(membrane) Aisle
End of
ACT 3
G
Aisle
+/K+
(Na
QuickTime™ and a
d eco mpres sor
are nee
ded to s ee this picture.
Pump)
Na+
Na+
G
Na+
G
Na+ Na+
G
G
G
G
(Glucose
Trans)
G
G
Na+
Na+
Na+
G
Na+
G
Na+
G
G
G
G
Na+
FRONT (inside cell)
(HUGE amounts of
Glucose)
Na+
http://www.stolaf.edu/people/giannini/flashanimat/transport/secondary%20active%20transport.swf
Questions to discuss in groups:
1. What is the direct energy source in a cell used
to transport glucose across the membrane against
its concentration gradient?
2. What happens to glucose when the Na/K pump
is blocked?
Question #4 from exam 2
What happens to glucose when the Na/K pump is blocked?
A. No effect on glucose transport
B. Glucose transport into the cell stops immediately
C. Glucose flows out of the cell
D. Glucose transport into the cell stops but not immediately
40
35
30
25
20
Q #4
15
10
5
0
A
B
C
D
Overall goal: Students will understand how cells
exchange substances with their environment.
Specific student learning outcomes for the unit:
1) Be able to explain the basis of selective membrane permeability
and distinguish between passive and active mechanisms of
membrane transport.
2) Be able to distinguish structural characteristics and functional
roles of the various membrane transport proteins (i.e. carriers,
channels, pumps)
3) Describe the physiological importance of membrane transport (i.e.
energy considerations)
4) Predict the outcomes of disruption of various membrane transport
systems