After lecture cool down activity… Reflect on your learning today Answer in complete sentences. 3 things/facts/ideas you learned today 2 things/facts/ideas that surprised you today 1 question, comment, concern, or query that you have about today’s lecture. READ Write your own 3 short answer questions – The more detailed or specific the better – Think of questions that might be on a test – Compare and Contrast – Synthesis (Create or Do something) Write the answers to your questions – Each answer should be a paragraph in length. – 5-7 Sentences – A good answer has 2-3 parts to it. S.U.: Review for Quiz STEP 1: (copy these words into your NB) – Monomer – Polymer – Dehydration Synthesis – Nucleotide – Amino Acid – Protein – Nucleic Acid – Carbohydrate – Monosaccharide STEP 2: – Rate the words on the left according to the scale below. Rating Scale: – 4 = I could easily teach it to someone else. – 3 = I think I know what it means, but I could use a review. – 2 = I have seen it or heard it before. – 1 = I have no idea what it means. STARTUP STEP 1: – Rate the words on the right according to the scale below. Rating Scale: 4 = I could easily teach it to someone else. 3 = I think I know what it means, but I could use a review. 2 = I have seen it or heard it before. 1 = I have no idea what it means. STEP 2: (copy all words in your notebook) – – – – – – – – – – Cell Nucleus Cell Membrane Cell Wall Ribosome Endoplasmic Reticulim Golgi Apparatus Mitochondria Chloroplast Lysosome Find your match. 3 minutes. Left Side: Right Side: Startup: Cell Theory FYI: Your test on cells, in approximately two weeks will be an in class essay. This will be worth 60 pts (3 quizzes) Below is your prompt: Answer the following question: – What is a cell? – Write down everything you know about cells. Be specific and site examples. For your stamp, you must be writing for at least 10 minutes today. See what you can do, and from this today we will build your knowledge into an essay! Cellular Biology What would you bring along if you were set afloat in the ocean, inside a clear Plexiglas sphere (10 m. in diameter)? You will be afloat for two years, how would you modify your sphere? II. Cell Theory A. Developed by Schleiden, Schwann and Virchow 1. All living things are composed of cells. 2. The cell is the functional unit of life. 3. New cells come from division of preexisting cells. III. Microscopes A. Light Microscopes 1. Advantages a. Can look at living things b. See in color c. Requires simple preparation d. Fairly inexpensive III. Microscopes 2. Disadvantages a. Specimens must be thin. b. Limited magnification (~2,000X) c. Resolution is poor on higher magnifications. B. Transmission & Scanning Electron Microscopes 1. Advantages a) Greater magnification (scanning 100,000 times and transmission 200,000). b) Scanning EM will give you a three dimensional view. Transmission EM Scanning EM 2. Disadvantages a) Can only look at dead things. b) Specimen preparation is time consuming and expensive. c) Objects appear black and white. d) Very expensive to purchase. What type of microscope took this picture? What kind of microscope took this picture? What type of microscope was used to take both of these pictures? V. Cell Size A. Why are cells so tiny? To maximize the surface area to volume ratio. B. What is the surface area and volume of a 4 cm cube? Surface Area (SA) = (h) x (w) x (# of sides) 4 x 4 x 6 = 96 cm2 Volume (V) = h x w x d 4 x4 x 4 = 64 cm3 h SA:V = 3:2 d w V. Cell Size C. What if we cut the cube into eight 2 cm cubes? Surface Area 2 x 2 x 6 x 8 = 192 cm2 Volume 2 x 2 x 2 x 8 = 64 cm3 SA:VOL = 3:1 cm V. Cell Size D. Why does SA/Vol ratio matter so much? a) Having twice the surface area and the same volume allows for a more efficient exchange of materials. Nutrients, wastes, oxygen, carbon dioxide and water. E. Another reason why cells are so small? a) Cell nucleus can only control so much cytoplasm (the materials inside the cell). VI. Classification of cells A. Classify cells according to their complexity 1. Prokaryotic cells (Bacteria) a) Most ancient and simple (unicellular). b) Do not have DNA organized in a nucleus. c) Do not have any membrane bound organelles, but they have ribosomes and cell membranes. VI. Classification of cells 2. Eukaryotic cells a) Generally larger and more complex than prokaryotes (can be single- or multi-celled organisms) b) Most cells are in this group c) Have DNA organized into chromosomes in a nucleus d) Have membrane bound organelles HOMEWORK: Quiz on Thursday Outline 7-3 Thursday Use the web and find pictures of organelles. SU: Compare and Contrast Eukaryotic to Prokaryotic Cells No talking, 10 minutes, Read pages 172-173 If you need a stamp, let Mr. Hagen know as he circulates and takes attendance. Create a T – Chart. List characteristics of prokaryotic and eukaryotic cells. Prokaryotic Cells Eukaryotic Cells CB Outline 7-3 Tomorrow Quiz Next Tuesday – Cell Theory and Cell Size – Eukaryotic Vs. Prokaryotic – Organelles HW: Look up pictures on the web of all the organelles and bring to class. ORGANELLE PROJECT 1 pt per description and 1 pt per picture 40 pts for the minimum, sky’s the limit! Description – 4-5 facts per description – If you copy the glossary, -.5 pts – +.5 for everything you bring from the web Picture – Detailed and in color – +1 if you bring a picture from the web. SU: CST Prep - Bacteria Which of the following structures is not found in bacteria? –Ribosome –Cytoplasm –Cell membrane –Nuclear Membrane CB Outline 7-3 Due TODAY Outline 7-4 Due on TUESDAY Reading quiz on 7-3 TUESDAY Organelle Project due in class on Monday House Tomorrow ORGANELLE PROJECT 1 pt per description and 1 pt per picture 40 pts for the minimum, sky’s the limit! Description – 4-5 facts per description – If you copy the glossary, -.5 pts – +.5 for everything you bring from the web Picture – Detailed and in color – +1 if you bring a picture from the web. SU: Eukaryotic Vs Prokaryotic Eukaryotic cells are differentiated from prokaryotic cells because eukaryotic cells –Are much smaller –Have permeable membranes –Have a higher rate of reproduction –Have a nuclei SU: Cell Membrane Functions of the cell membrane include –A. Protecting cells –B. Supporting cells –C. Controlling what enters and leaves cells –D. All of the above CB Organelle Project Due Today Outline 7-4 Due by Friday –Make sure outline 7-3 is due by Wednesday Quiz on Cell Organelles Wednesday Cell Membrane 1. 2. 3. Thin flexible barrier made of a lipid bilayer that surrounds cells Lipid bilayer – 2 layers of lipids with proteins embedded in it with CHO chains attached Regulates what comes in & out of cell Protection support Cell Wall Rigid layer outside of cell membrane Made of carbohydrates & protein Found only in plant cells 1. Provides support & protection to the cell Nucleus 1. 2. Large organelle surrounded by a nuclear envelope Contains DNA Controls all cell activities DNA made here Nucleolus Small dense region inside the nucleus 1. Makes ribosomes Chromatin Granular material that consists of DNA Found in the nucleus 1. Condenses to form chromosomes when the cell divides Chromosome 1. 2. Thread like structures that contain genetic information Passes genetic information from one generation to the next Units of heredity Cytoplasm Jellylike substance outside of nucleus but enclosed by the cell membrane Where all organelles are found 1. Gives cell its shape Mitochondria 1. 2. 3. Peanut shaped organelle enclosed by 2 membranes (inner & outer) Converts the chemical energy (sugar such as glucose) stored in food into compounds (ATP) that the cell can use for energy “powerhouse” “energy maker” Golgi Complex Stacks of membranes closely packed together 1. modify, sort, & package proteins & other materials from the endoplasmic reticulum (ER) for storage in the cell or for secretion out of the cell Ribosome Small particles of RNA & protein Found throughout the cytoplasm 2 types – free & attached 1. Makes proteins Smooth Endoplasmic Reticulum Smooth (ER) 1. 2. Internal membrane system without ribosomes Contains enzymes Makes lipids Transports materials inside cell Smooth ER Rough Endoplasmic Reticulum (ER) Internal membrane system with ribosome's attached Surrounds the nucleus 1. Synthesis of proteins from ribosomes Centrioles Located near the nucleus Made of microtubules 1. Helps with cell division Cytoskeleton 1. 2. 3. Network of protein filaments Made of microfilaments & microtubules Help support cell Maintains its shape Helps materials move within the cell Lysosome Small organelle filled with enzymes 1. Eats or breakdown lipids, carbohydrates, & proteins Removes junk from cell Breakdown old organelles 2. 3. Chloroplast Large stacks of membranes Found only in plant cells 1. Capture energy from sunlight & converts it into chemical energy - Photosynthesis Vacuole Storage saclike structures Found only in plant cells 1. Stores water, food, salts, Carbohydrates, proteins & wastes Contractile vacuole 1. 2. Specialized vacuole Pumps excess water out of cells Helps maintain homeostasis HW Organelle project is late Old outlines are late Quiz on Wednesday on organelles Outline 7-4 due Friday SU: Rough Endoplasmic Reticulum Part of the endoplasmic reticulum is called rough because its surface –A. has holes for secreting proteins. –B. contains stacklike membranes. –C. is covered with ribosomes. –D. attracts proteins. CB QUIZ TOMORROW Outline 7-4 due on Friday Organelle Project needs to be turned in ASAP. Everyday late you loose one letter grade. Today’s Assignment Create flash cards for the cell organelles. Take a piece of paper and fold it in half, lengthwise, and make two more folds so you have 8 folds per page. Cut each page into 8 flash cards. Flash Cards Cell Membrane Cell Wall Nucleus Nucleolus Chromosome Cytoplasm Mitochondria Cytoskeleton (microtubule and microfilament) Lysosomes Chloroplasts Golgi Apparatus Ribosome Smooth Endoplasmic Reticulum Rough Endoplasmic Reticulum Vacuole Centriole Organelle SU: Review for Quiz If you do not have flash cards from yesterday. Complete the table to the right. Cell membrane Cell wall Nucleus Nucleolus Chromatin Chromosome Cytoplasm Ribosome Mitochondria Golgi complex Smooth ER Rough ER Centriole Cytoskeleton Lysosome Chloroplast vacuole Description Function CB Outline 7-4 Due on Friday Notebook Check on Monday 10/27 Quiz Today – 20 min Free writing on the descriptions of cell organelles. – 2 min with notebook only, no project, no flash cards or anything. QUIZ Describe all the parts of a cell. Size, Shape, Function, Job description in the cell. You may draw pictures. 1pt for every fact. HW Outline 7-4 Organelle Project after today is a C or less. NB check 3 – 80pts Minimum, Monday Below are unused or slides not used in class yet but contain very important information which could be useful VII. Eukaryotic Cell Structure How is the cell like a factory? Two main parts: A. Nucleus Main control center of the cell 1. Coordinates cell’s activities 2. Contains cell’s DNA (chromatin) and nucleolus (ribosome assembly) 3. Surrounded by a porous nuclear envelope Allows materials (proteins, RNA, other molecules) that act as messengers to move in and out of cell Nucleus Nucleolus Small dense region inside the nucleus 1. Makes ribosomes VII. Eukaryotic Cell Structure B. Cytoplasm Area outside the nucleus that contains the cell organelles and where the nucleus’ instructions are carried out 1. Endoplasmic reticulum is a membranous labyrinth (two types) a) Rough ER is covered with ribosomes Ribosomes assemble proteins according to instructions from nucleus (“the boss”) VII. Eukaryotic Cell Structure b) Smooth ER has no ribosomes • Contain special enzymes that synthesize lipids, detoxify drugs and metabolize carbohydrates In which organs would we find a lot of Smooth ER? 2. Golgi Apparatus • Proteins made by the ER are sent here to be modified, sorted and packaged for storage in the cell or secretion outside the cell From here, proteins are “warehoused”, then “shipped” to their final destinations VII. Eukaryotic Cell Structure Lysosomes • Small membrane-enclosed sac of enzymes that digest proteins, lipids and carbs into smaller molecules that can be used by the cell • Break down old organelles and pathogens Cell’s “clean-up” crew 4. Vacuoles • Membrane-enclosed sac that performs diverse functions depending on the cell type (i.e. storage, support, water balance) Storage area for water, salt, proteins and carbs 3. VII. Eukaryotic Cell Structure 5. Mitochondria • Enclosed by two membranes • Site of cellular respiration • Convert chemical energy stored in food into compounds the cell can use (ATP) Cell’s “power house” or energy source VII. Eukaryotic Cell Structure 6. Chloroplasts • Enclosed by two membranes • Site of photosynthesis • Contain chlorophyll (green pigment) Only found in what type of cell? Plant cell’s “power house” or energy source VII. Eukaryotic Cell Structure 7. Cell “Skin” • • Animals have cell membrane Plants have cell wall AND cell membrane VII. Eukaryotic Cell Structure 8. Cytoskeleton • Network of protein filaments that helps maintain cell shape and aid in movement Protein filaments: • Microfilaments are threadlike structures made of actin • Microtubules are hollow structures made of tubulins ; includes centrioles in animal cells Cell’s support structure and transportation system VIII. Endosymbiotic Theory 1. Developed by Lynn Margulis. 2. Single-celled organisms engulfed another smaller single-celled organisms. 3. Digestion did not occur. The two organisms lived symbiotically. 4. Over millions of generations, these engulfed organisms became organelles. 5. Evidence: a) Mitochondria and Chloroplasts have their own DNA b) They also divide independent of the cell.