General Microbiology Laboratory
Introduction to Micro Lab
‫‪Welcome to Micro Lab‬‬
‫قال رسول هللا صلى هللا عليه وسلم‬
‫(ال عدوى وال طرية وال هامة وال ص َفر‪ ،‬وفر من اجملذوم كما تفر من‬
‫األسد)‬
Welcome to Micro Lab
The goal of the laboratory is to expose
students to the wide variety of life in the
microbial world.
Although the study of microbiology includes
bacteria, viruses, algae and protozoa, this lab
will concentrate primarily on the bacteria
Microbiological techniques are important in
preparing the students for the much harder task of
identifying the pathogenic microorganisms in a
clinical and environmental specimen.
Each experiment with a brief theoretical introduction
revealing the theoretical basis on which the
experiment is based on, so that there will be a
strong conjunction between the practical and
theoretical sessions.
Also included in this manual, the safety precautions
that each one in the field of microbiology should
know and practice
Bacteria belong to the kingdom Monera. This kingdom contains
more biological diversity than all other kingdoms combined.
Most people tend to associate bacteria with disease, but less
than ten percent of all bacteria cause disease.
Many bacteria cannot even live at the temperatures found in
and on the human body.
In this lab, most of the bacteria with which we will be working
are non-pathogenic (do not cause disease). However, some
of the bacteria are opportunistic; that is, they can cause
disease in an ill or injured person.
Therefore, treat all bacteria as if they are pathogenic (cause
disease).
Laboratory Safety Rules
1. Wear a lab coat in lab. We will be working
with a variety of materials that can cause
permanent stains on some fabrics. Also, a lab
coat can help protect from accidental
contamination by microorganisms
No eating or drinking during lab. Many
pathogens spread by ingested food and drink.
In addition, food can carry microorganisms
that might contaminate laboratory cultures
Laboratory Safety Rules
Keep long or fluffy hair tied up and out of the
way. Hair can contaminate and be
contaminated by microbial cultures.
Always wear shoes in lab
Thoroughly wash your hands with soap and
water before and after lab. Thorough and
frequent hand washing easily and effectively
controls the spread of many pathogens.
Laboratory Safety Rules
Clean the lab bench with disinfectant
before and after lab. This helps to
prevent contamination of cultures,
books, clothing, etc
Keep the lab bench free of
unnecessary materials. Don't use the
lab bench as a storage area for coats,
books, etc.
Do not take cultures from the lab area
Laboratory Safety Rules
 Dispose all contaminated materials in autoclave bags.
When in doubt, ask the instructor.
 Immediately report all accidents and spills to the
instructor. Cover spills with disinfectant-soaked paper
towels for at least 15 minutes before disposing them
 Read all assigned materials before the lab session.
Experiments will go smoother and have greater chances
of success when you know what you will be doing ahead
of time
Laboratory Safety Rules
Treat all microbial cultures as if they are
pathogens. Better safe than sorry
When in doubt, ask the instructor.
The only stupid questions are those that
are intended as such.
NOTES
1. Personal belongings are not to be
stored in the laboratory.
2. You will be assigned to a group
consisting of four students and you will
work together in a semester long project
Lab requirements
Lab coat
Matches or lighter
Soap
Waterproof permanent marker
Small or medium size towel
Sealable plastic bag
Rubber band
General Microbiology Manual
Acquaint yourself with microbiology lab
Microscope
Staining solutions
Dettol
Microscopic slides
Bunsen burner
Acquaint yourself with microbiology lab
Forceps
Wooden tongue depressor and swabs
Sterile cups
Syringes
Normal saline
Acquaint yourself with microbiology lab
Incubator
Autoclave
Acquaint yourself with microbiology lab
Laminar flow Hood
Acquaint yourself with microbiology lab
Culture Plates or Petri
Dishes
Plastic Loops and Needles
Flaming Nichrome loop
Acquaint yourself with microbiology lab
Anaerobic Jar
Candle jar
Acquaint yourself with microbiology lab
Anaerobic station
Acquaint yourself with microbiology lab
Enterotube
API 20 E
Acquaint yourself with microbiology lab
Liquid media in tubes
Solid media in tubes
Acquaint yourself with microbiology lab
Different forms of slants
Acquaint yourself with microbiology lab
Blood culture bottles
Acquaint yourself with microbiology lab
Antibiotic disks
Now
Try to Identify all the tools and
Equipment around you
END OF LECTURE