Midterm Review CHAPTER 1 Introduction to Biology THE STEPS OF THE SCIENTIFIC METHOD 1. 2. MAKE AN OBSERVATION FORM A HYPOTHESIS a) A PREDICTION(EDUCATED GUESS?) 3. TEST THE HYPOTHESIS WITH AN EXPERIMENT a) EXPERIMENTS SHOULD HAVE ONLY 2 VARIABLE b) INDEPENDENT VARIABLE –the thing that YOU change c) DEPENDENT VARIABLE- the thing that changes in response d) A CONTROL IS SOMETHING HELD CONSISTENT FOR ALL 4. COLLECT AND ANALYZE DATA a) WE USE MATHEMATICAL-QUANTITATIVE DATA I. GRAPHS, TABLES AND MEASURES LIKE AVERAGES, MEDIANS.. 5. FORM A CONCLUSION a) DOES THE DATA SUPPPORT YOUR HYPOTHESIS 6. SHARE YOUR DATA* a) EXPERIMENTAL DATA SHOULD BE REPLICABLE 3 LEVELS OF SCIENTIFIC CERTAINTY 1. A HYPOTHESIS IS THE LOWEST LEVEL 1. A PREDICTION TO EXPLAIN YOUR OBSERVATION (EDUCATED GUESS?) 2. A THEORY IS A CONSENSUS WITHIN THE SCIENTIFIC COMMUNITY 1. A GENERAL EXPLANATION FOR A BROAD RANGE OF DATA 2. USES DATA FROM A VARIETY OF SOURCES FOR SUPPORT 3. EXAMPLE –THE EXTINCTION OF DINOSAURS 3. A LAW HAS NO SIGNIFICANT DATA TO OPPOSE IT 1. DOES THE DATA SUPPPORT YOUR HYPOTHESIS 2. EXAMPLE – E = mc2 THE BRACHES OF BIOLOGY – BIOLOGY IS THE THE STUDY OF ORGANISMS FOR EACH BRANCH OF BIOLOGY WE STUDY HOW ORGANISMS.. 1. ECOLOGY – INTERRACT WITH THE ENVIRONMENT 2. CELL BIOLOGY- CELLS AND THEIR STRUCTURES 3. GENETICS – HOW TRAITS ARE INHERITED OR HEREDITY 4. BIOCHEMISTRY- CHEMISTRYOF LIFE OR METABOLIC PROCESSES 5. EVOLUTIONARY BIOLOGY –HOW LIFE EVOLVES 6. MICROBIOLOGY – MICROSCOPIC ORGANISMS 7. BOTANY - PLANTS 8. ZOOLOGY – ANIMALS 9. PHYSIOLOGY – HUMAN BODY THE 7 CHARACTERISTICS OF LIFE AN ORGANISM: IS A LIVING THING CAPABLE OF CARRYING ON ALL THE PROCESS OF LIFE ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. CELLULAR ORGANIZATION 2. HOMEOSTASIS 3. HEREDITY 4. RESPONSIVENESS 5. REPRODUCTION 6. GROWTH AND DEVELOPMENT 7. METABOLISM THE 7 CHARACTERISTICS OF LIFE: ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. CELLULAR ORGANIZATION a) ORGANISMS ARE EITHER UNICELLULAR OF MULTICELLULAR I. UNICELLULAR -1 CELLED II. MULTICELLULAR – MORE THAN 1 CELL b) THEY ARE PROKARYOTIC OR EUKARYOTIC PROKARYOTIC –LACK A NUCLEUS OR ORGANELLES II. EUKARYOTIC – HAVE NULCEUS AND ORGANELLES I. THE 7 CHARACTERISTICS OF LIFE: ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1.HOMEOSTASIS 1. THE ABILITY TO MAINTAIN A STABLE INTERNAL ENVIRONMENT 2. EXAMPLE –THERMOREGULATION, BLOOD PRESSURE, BLOOD GLUCOSE THE 7 CHARACTERISTICS OF LIFE– ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. HEREDITY 1. ORGANISMS PASS THEIR TRAITS TO THEIR OFFSPRING 1. THEY USE THE MOLECULE DNA ORGANIZED IN CHROMOSOMES 2. THESE INHERITED TRAITS CHANGE OVER TIME – EVOLVE 1. SPECIES TRAITS ARE ENCODED IN DNA WHICH CHANGES THE 7 CHARACTERISTICS OF LIFE– ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. RESPONSIVENESS 1. ORGANISMS RESPOND TO THE ENVIRONMENT 1. INDIVIDUALS RESPOND WITH BEHAVIORS 2. SPECIES RESPOND BY EVOLVING THE 7 CHARACTERISTICS OF LIFE– ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. REPRODUCTION a. b. SEXUAL REPRODUCTION 1) THE EXCHANGE AND COMBINATION OF DNA 2) SEX CELLS (GAMETES)COMBINE TO FORM NEW INDIVIDUALS 3) HIGH LEVEL OF VARIATIONS ASEXUAL REPRODUCTION 1) THE REPLICATION OF PARENTS TO FORM DAUGHTER CELL 2) OFFSPRING IDENTICAL TO PARENTS 3) EXAMPLE –BINARY FISSION THE 7 CHARACTERISTICS OF LIFE– ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. GROWTH AND DEVELOPMENT a) ORGANISM GROW BY MITOSIS b) ORGANISMS DEVELOP THROUGH CELLULAR DIFFERENTIATION THE 7 CHARACTERISTICS OF LIFE– ALL ORGANISMS SHARE THE FOLLOWING TRAITS… 1. METABOLISM 1. THE SUM OF ALL CHEMICAL REACTIONS 2. IN LIVING SYSTEMS THE CARBON CYCLE IS THE FUNDAMENTAL PROCESS a) PHOTOSYNTHESIS I. CO2 + H2O --- C6H12O6 + O2 b) CELL RESPIRATION I. C6H12O6 + O2 --- CO2 + H2O Midterm Review CHAPTER 18 CLASSIFICATION THE CLASSIFICATION OF ORGANISMS 1. TAXONOMY -THE SCIENCE OF CLASSIFYING ORGANISMS a) CARL LINNAEUS-THE FATHER OF MODERN TAXONOMY b) BINOMIAL NOMENCLATURE –SCIENTIFIC NAME I. A 2 NAME SYSTEM II. Genus species or Genus species III. SCIENTIFIC NAMES ARE GIVEN IN LATIN c) CLADISTICS IS THE ORGANIZATION OF ORGANISMS ON THE BASIS OF SHARED TRAITS I. WE CAN USE A CLADOGRAM TO LINK RELATED ORGANISMS THE CLASSIFICATION OF ORGANISMS 1.TAXONOMY -THE 3 DOMAIN SYSTEM a) ORGANISMS ARE IDENTIFIED BY CHARACTERISTICS I. ARCHAEBACTERIA- ANCIENT BACTERIAEXTREMOPHILES II. BACTERIA- STREPTOCOCCUS, STAPHYLOCOCCOUS III. EUKARYA- PROTISTA, FUNGI, PLANTAE, ANIMALIA THE CLASSIFICATION OF ORGANISMS 1.7 LEVELS OF THE MODERN TAXONOMY a) DOMAIN b) KINGDOM c) PHYLUM d) CLASS e) ORDER f) FAMILY g) GENUS h) SPECIES THE CLASSIFICATION OF ORGANISMS 1.THE HUMAN CLASSIFICATION a) DOMAIN – EUKARYA b) KINGDOM - ANIMALIA c) PHYLUM - CHORDATA d) CLASS - MAMMALIA e) ORDER - PRIMATE f) FAMILY - HOMINIDAE g) GENUS - HOMO h) SPECIES - SAPIENS Midterm Review CHAPTER 3 CHEMISTRY OF LIFE THE CHEMISTRY OF LIFE 1. MATTER-IS COMPOSED OF ATOMS a) ATOMS ARE COMPOSED OF SUBATOMIC PARTICLES I. PROTON a) b) c) FOUND IN THE NUCLEUS POSITVE CHARGE +1 MASS IS 1 ATOMIC MASS UNIT (AMU) II. NEUTRON a) b) c) FOUND IN THE NUCLEUS NEUTRAL CHARGE MASS IS 1.01 ATOMIC MASS UNIT (AMU) III. ELECTRON a) b) c) FOUND IN THE ORBITAL ENERGY SHELLS NEGATIVE CHARGE -1 MASS IS 0.01 ATOMIC MASS UNIT (AMU) THE CHEMISTRY OF LIFE 1. AN ELEMENT IS A TYPE OF ATOM a) AN ELEMENT IS SUBSTANCE MADE UP OF THE SAME TYPE OF ATOMS I. ELEMENTS OF THE SAME SUBSTANCE HAVE THE SAME NUMBER OF PROTONS GIVEN BY THE ATOMIC NUMBER a) b) c) CARBON 6 OXYGEN 8 HYDROGEN 1 II. SOME ATOMES OF THE SAME SUBSTANCE MAY HAVE DIFFERENT NUMBERS OF NEUTRON a) b) c) CARBON12 -6 PROTONS 6 NEUTRON CARBON14 -6 PROTONS 8 NEUTRON THEY ARE CALLED ISOTOPES OF CARBON THE CHEMISTRY OF LIFE 1. THE PERIODIC TABLE ORGANIZES ALL ATOMS ITS CONTAINS THE FOLLOWING FOR EACH ELEMENT a) CHEMICAL SYMBOL I. AN ABBREVIATION OF AN ATOMS NAME a) b) C-CARBON, H-HYDROGEN SOME ARE UNUSUAL Na- Sodium, K-Potassium b) ATOMIC NUMBER I. AN ATOMS PROTON NUMBER a) b) CARBON HAS 6 PROTONS –ATOMIC NUMBER 6 HYDROGEN HAS 1 PROTON – ATOMIC NUMBER 1 c) ATOMIC MASS I. THE COMBINATION OF THE MASS OF PROTONS AND NEUTRONS a) b) CARBON – 6 PROTONS + 6 NEUTRON = ATOMIC MASS 12 SOME ARE UNUSUAL Na- Sodium, K-Potassium THE CHEMISTRY OF LIFE 1. CHEMICAL BONDS – ATOMS COMBINE THERE ARE 3 IMPORTANT BONDS a) COVALENT BOND I. BASED ON THE SHARING OF ELECTRONS a) b) c) FORM MOLECULES DRIVEN BY THE OCTET RULE- WHICH STATES “MOST ATOMS REQUIRE 8 ELECTRONS IN THE OUTERMOST ORBITAL SHELL-(VALENCE SHELL) EXAMPLES INCLUDE CO2 , C6H12O6, O2 b) IONIC BONDS I. BASED ON THE DONATION OR ACCEPTANCE OF ELECTRONS a) b) c) d) FORM IONIC COMPOUNDS ATTRACTIONS BETWEEN CHARGED ATOMS CALLED IONS DRIVEN BY THE ELECTRONEGATIVITY OF AN ELEMENT EXAMPLES INCLUDE NaCl, NaOH, HCl c) HYDROGEN BONDS I. BASED ON ATTRACTIONS BETWEEN H+ ATOMS AND OXYGEN a) IMPORTANT IN WATER, DNA , & PROTEIN STRUCTURE THE CHEMISTRY OF LIFE WATER - LIFE IS DEPENDENT UPON THE CHARACTERISTICS OF WATER a) WATER IS A POLAR MOLECULE I. IT IS A MOLECULE WITH IONIC CHARACTER a) b) c) OXYGEN IN WATER CARRIES A PARTIAL (-) CHARGE HYDROGENS IN WATER CARRIES A PARTIAL (+) CHARGE ADJACENT MOLECULES ARE ATTRACTED TO ONE ANOTHER b) LIQUID WATER IS LESS DENSE THAN SOLID WATER I. ICE FLOATS a) b) c) d) FLOATING ICE PREVENTS LAKES AND OCEANS FROM FREEZING COMPLETELY AND ALLOWS ORGANISMS TO LIVE IN COLD CONDITIONS ATTRACTIONS BETWEEN CHARGED ATOMS CALLED IONS DRIVEN BY THE ELECTRONEGATIVITY OF AN ELEMENT EXAMPLES INCLUDE NaCl, NaOH, HCl c) WATER IS STICKY I. IT IS BOTH ADHESIVE AND COHESIVE a) b) COHESION WATER STICKS TO ITS SELF ADHESIONWATER STICKS TO OTHER POLAR MOLECULES d) WATER IS THE UNIVERSAL SOLVENT I. IT DISSOLVES POLAR MOLECULES THE CHEMISTRY OF LIFE WATER –THE UNIVERSAL SOLVENT a) A SOLUTION CONTAINS BOTH A SOLVENT AND SOLUTE I. A SOLUTE IS THE SOLID DISSOLVED IN SOLUTION II. THE SOLVENT IS THE LIQUID COMPONENT OF A SOLUTION a) b) c) POLAR SOLVENTS DISSOLVE POLAR COMPOUNDS a) EXAMPLE WATER AND SUGAR NON-POLAR SOLVENTS DISSOLVE NON-POLAR COMPOUNDS a) EXAMPLE GASOLINE AND OIL SOLUTIONS WHERE WATER IS THE SOLVENT ARE CALLED AQUAEOUS THE CHEMISTRY OF LIFE WATER –THE UNIVERSAL SOLVENT a) ACIDS AND BASES ARE AQUAEOUS SOLUTIONS b) THE pH scale measures H+ ion concentration I. ACIDS –LOW pH a) b) H+ DONORS EXAMPLES INCLUDE LEMON JUICE, HCl II. BASES - HIGH pH a) b) H+ ACCEPTORS EXAMPLES INCLUDE, NaOH, OVEN CLEANER, LYE, AMMONIA THE CHEMISTRY OF LIFE BUFFERS – IONIC COMPOUNDS IN LIVING SYSTEMS a) NEUTRALIZE ACIDS AND BASES b) AN IMPORTANT EXAMPLE OF HOMEOSTASIS c) THEY CAN ACT AS H+ DONORS OR ACCEPTORS d) YOU HAVE BUFFERS IN YOUR BLOOD THAT ALLOW YOU TO MAINTAIN CONSTANT pH e) ANTACIDS ARE EXAMPLES THE CHEMISTRY OF LIFE BIOCHEMISTRY- THERE ARE 4 MAJOR CLASSES OF BIOLOGICALLY ACTIVE MOLECULES a) CARBOHYDRATES (SUGARS OR SACCHARIDES) I. II. OFTEN POLYMERS OF GLUCOSE THE MAJOR AND 1ST SOURCE OF ENERGY IN LIVING THINGS b) LIPIDS (FATS) I. II. ARE THE MOST DENSE ENERGY STORAGE MOLECULES FORM ALL CELL MEMBRANES c) PROTEINS I. II. POLYMERS OF AMINO ACIDS JOINED BY PEPTIDE BONDS ALL ENZYMES ARE PROTEIN d) NUCLEIC ACIDS I. II. FOUND IN THE NUCLEUS AS DNA OR RNA POLYMERS OF NUCLEOTIDES THE CHEMISTRY OF LIFE CARBOHYDRATES I. COMMONLY CALLED SUGARS OR SACCHARIDES (-OSEs) II. CAN BE FOUND AS MONO, DI, OR POLYSACCHARIDES a) b) c) MONOSACCHARIDE- GLUCOSE DISACCHARIDE- SUCROSE, LACTOSE POLYSACCHARIDE- STARCH, CELLULOSE, GLYCOGEN III. THE 1ST SOURCE OF ENERGY IN LIVING THINGS IV. FORM STRUCTURAL ELEMENTS IN ORGANISM LIKE THE CELL WALL a) b) WOOD IS CELLULOSE A BUGS SHELL IS MADE OF CHITIN THE CHEMISTRY OF LIFE LIPIDS I. POLYMERS OF FATTY ACIDS II. ARE USED FOR LONG TERM ENERGY STORAGE a) EXAMPLES CHOLESTEROL III. FORM ALL CELL MEMBRANES a) b) PHOSPHOLIPID PREVENTS THE MOVEMENT OF H2O IN OR OUT OF THE CELL IV. ACT AS HORMONES a) TESTOSTERONE & ESTROGEN THE CHEMISTRY OF LIFE PROTEINS a) POLYMERS OF AMINO ACIDS JOINED BY PEPTIDE BONDS b) ALL ENZYMES ARE PROTEIN c) PROTEINS HAVE IMPORTANT ROLES AS I. ENZYMES (-ASEs) I. II. EXAMPLE AMYLASE, HYDROLASE, ATPase HORMONES- like INSULIN, SEROTONIN III. STRUCTUAL ELEMENTSKERATIN, COLLAGEN THE CHEMISTRY OF LIFE NUCLEIC ACIDS I. CARRY HEREDITARY INFORMATION IN THE FORM OF GENES II. FOUND IN THE NUCLEUS a) FORMS AN ALPHA DOUBLE HELIX III. POLYMERS OF NUCLEOTIDES a) b) c) CONTAIN A SUGAR –RIBOSE OR DEOXYRIBOSE NITROGENOUS BASE PHOSPHATE GROUP THE CHEMISTRY OF LIFE MATTER AND ENERGY TRANSFORMATIONS 1. CHEMICAL REACTIONS ARE SYMBOLIZED IN EQUATIONS REACTANTS------PRODUCTS A + B -------------- C + D 2. CHEMICAL REACTIONS STORE AND RELEASE ENERGY a) b) ENDOTHERMIC REACTIONS–NEED ENERGY TO PROCEED EXOTHERMIC REACTIONS – GIVE OFF ENERGY AS THEY PROCEED 3. ACTIVATION ENERGY IS REQUIRED FOR A CHEMICAL REACTION TO PROCEED 4. ENZYMES ARE BIOLOGICAL CATALYSTS THAT LOWER ACTIVATION ENERGY a) b) THIS SPEED CHEMICAL REACTIONS THIS ALLOWS THE CHEMICAL REACTIONS NECESSARY FOR LIFE THE CHEMISTRY OF LIFE ENZYMES ARE BIOLOGICAL CATALYSTS ACTIVATION ENERGY IS REQUIRED FOR A CHEMICAL REACTION TO PROCEED ENZYMES ARE BIOLOGICAL CATALYSTS THAT LOWER ACTIVATION ENERGY a) THIS SPEEDS CHEMICAL REACTIONS b) THIS ALLOWS THE CHEMICAL REACTIONS NECESSARY FOR LIFE THE CHEMISTRY OF LIFE ENZYMES ARE BIOLOGICAL CATALYSTS 1. 2. 3. 4. ENZYMES ARE PROTEINS WITH A SPECIFIC 3DIMENSIONAL STRUCTURE ENZYMES LOWER ACTIV ATION ENRGY BY BINDING SUBSTRATES AT THEIR ACTIVE SITES ENZYMES CATALYZE REACTIONS WITHOUT BEING CHANGED OR USED UP ENZYME ACTIVITY CAN BE INFLUENCED BY THE ENVRONMENT THE CHEMISTRY OF LIFE ENZYMES ARE BIOLOGICAL CATALYSTS 1. ENZYME ACTIVITY CAN BE INFLUENCED BY THE ENVIRONMENT a) CHANGES IN pH b) CHANGES IN TEMPERATURE c) CHANGES IN ENZYME OR SUBSTRATE CONCENTRATION Midterm Review CHAPTER 7 CELLULAR ORGANIZATION CHAPTER 7 CELLULAR STRUCTURE THE DISCOVERY OF CELLS 1. (1665) ROBERT HOOK: “CELLS” 2. (1695) ANTON VON LEEUWENHOEK: “ANIMACULES” 3. SCHLIEDEN: PLANTS ARE MADE OF CELLS 4. SCHWANN: ANIMALS ARE MADE OF CELLS 5. VIRCHOW: ALL CELLS COME FROM PREXISTING CELLS CHAPTER 7 CELLULAR STRUCTURE THE CELL THEORY 1. 2. 3. ALL LIVING THINGS ARE MADE OF ONE OR MORE CELLS” CELLS ARE THE BASIC UNIT OF STRUCTURE AND FUNCTION ALL CELLS COME FROM PREXISTING CELLS CELL BIOLOGISTS USE MICROSCOPES 1. THIS IS A COMPOUND LIGHT MICROSCOPE 1. COMPOUND DUE TO MULTIPLE LENSES 2. LIGHT MUST PASS THROUGH THE OBJECT BEING OBSERVED 3. THE EYEPIECE OR OCULAR LENS IS ON TOP 4. THE OBJECTIVE LENSES ARE DOWN NEAR THE OBJECT CELL BIOLOGISTS USE MICROSCOPES 1. MAGNIFICATION a) 2. IS THE ABILITY OF A MICROSCOPE TO MAGNIFY OR ENLARGE AN OBJECT RESOLUTION a) IS A MEASURE OF HOW CLEARLY DETAILS CAN BE SEEN OTHER TYPES OF MICROSCOPES 1. ELECTRON MICROSCOPE a) 2. GIVES EXTREMELY HIGH MAGNIFICATION AND RESOLUTION SCANNING TUNNELING MICROSCOPE a) GIVES EXTREMELY HIGH MAGNIFICATION AND 3D IMAGES CELL SIZE AND SHAPE WHAT DETERMINES A CELLS SIZE 1. A HIGH SURFACE TO VOLUME RATIO 1. THE GREATER THE SURFACE AREA, MORE STUFF GETS IN Spinal Neuron-Over 3 feet long AND OUT OF CELL 1. GREATER THE VOLUME, LESS STUFF GETS IN OR OUT; CELL 2. STARVES OR IS POISONED CELL SIZE VARIES WITH FUNCTION Sperm Cell- 25 Micrometers CELL STRUCTURE 3 Major Parts of Cell 1. PLASMA MEMBRANE: a) CONTROLS PASSAGE OF MATERIALS IN OR OUT OF CELL 2. NUCLEAR REGION: a) CONTROLS CELLS ACTIVITIES; b) CONTAINS DNA & RNA 3. CYTOPLASM : a) ORGANELLES AND CYTOSOL INTERNAL ORGANIZATION & TYPES OF CELLS PROKARYOTIC CELLS: CELLS WITHOUT A NUCLEUS OR OTHER ORGANELLES EUKARYOTIC CELLS: CELLS WITH A NUCLEUS AND OUTER CELL MEMBRANE WHICH ALLOWS MOLECULES TO PASS IN AND OUT EUKARYOTIC CELLS CONTAIN “LITTLE ORGANS” CALLED ORGANELLES EACH ORGANELLE PERFORMS SPECIFIC FUNCTIONS CELLULAR ORGANIZATION Cells are organized into tissues Nerve Tissue is composed of astrocytes and neurons Tissues are organized into organs The heart is made of muscle and connective tissue Organs are organized into organ Systems The respiratory system includes the lungs, trachea, nasal passages, diaphragm muscle and blood vessels Organs Systems are organized into an Organism We are composed of our body systems including; Cardiovascular, rproductive, digestive, nervous…etc. PLANT & ANIMAL CELL ORGANELLES 1. CYTOPLASM a) ALL THE AREA BETWEEN THE CELL MEMBRANE AND THE NUCLEUS AND CONTAINS ALL OF THE ORGANELLES WITHIN THE CELL 2. RIBOSOMES a) MAKE PROTEINS FROM AA; FOUND ON ER OR IN CYTOSOL b) MADE IN THE NUCLEOLUS OF RRNA 3. ROUGH ER a) MAKES PROTEINS AND TRANSPORTS THEM TO OTHER PARTS OF THE CELL VIA VESICLES (LITTLE PACKAGES) PLANT & ANIMAL CELL ORGANELLES 4. SMOOTH ER a) TRANSPORTS PROTEINS b) SYNTHESIZES LIPIDS 5. GOLGI APPARATUS a) FOUND CLOSE TO ER. b) TAKES PROTEIN FROM ER AND MODIFIES THEM TO MAKE THEM WORK; c) SENDS THEM ON THEIR WAY (MAILROOM OF THE CELL) PLANT & ANIMAL CELL ORGANELLES 6. MITOCHONDRION a) POWERHOUSE OF THE CELL. TAKES IN FOOD; CONVERTS IT TO ATP, WHICH IS BROKEN DOWN FOR ENERGY. b) SOME CELLS HAVE MORE MITOCHONDRIA THAN OTHERS CRISTAE: FOLDS IN THE INNER MEMBRANE OF MITOCHONDRIA TO INCREASE SURFACE AREA 7. NUCLEUS a) CONTROLS AND COORDINATES CELL’S ACTIVITIES. CONTAINS CHROMATIN (DNA). DNA CONVERTED TO RNA AND STORED IN NUCLEOLUS. RNA SENT OUT TO b) c) CELL AS A MESSENGER SURROUNDED BY NUCLEAR ENVELOPE NUCLEAR PORES ALLOW RNA TO LEAVE NUCLEUS PLANT & ANIMAL CELL ORGANELLES 8. CYTOSKELETON: SCAFFOLDING THAT GIVES CELL SHAPE a) MICROFILAMENTS: USED FOR MUSCLE CONTRACTION b) MICROTUBULES: THICKER, MOVE ORGANELLES c) CILIA AND FLAGELLA I. HELP SOME CELLS MOVE AROUND ≈ II. LINING OF RESPIRATORY TRACT 9. LYSOSOMES: a) CONTAIN DIGESTIVE ENZYMES b) BREAKS DOWN GLUCOSE c) CAN RUPTURE AND KILL CELL Midterm Review CHAPTER 8 CELLULAR TRANSPORT Structure of Plasma Membrane SELECTIVELY PERMEABLE: ALLOWS SOME MOLECULES IN; KEEPS OTHERS OUT PHOSPHOLIPID BILAYER: POLAR HEADS ON OUTSIDE AND INSIDE OF CELL; NONPOLAR TAILS ON INSIDE OF MEMBRANE POLAR: HYDROPHILIC: WATER LOVING NONPOLAR:HYDROPHOBIC: WATER HATING Molecules on the Plasma Membrane 1. 2. 3. 4. 5. GLYCOPROTEINS: ACT AS RECEPTORS; MOLECULES ATTACH TO CELL; TELL IT WHAT TO DO CHOLESTEROL GIVES MEMBRANE SHAPE; RIGIDITY RECEPTOR PROTEINS BIND HORMONES & OTHER SUBSTANCES COMING IN FROM OUTSIDE THE CELL RECOGNITION PROTEINS PROTEINS ON MEMBRANE THAT ARE USED FOR RECOGNITION BY EXTRA-CELLULAR SUBSTANCES ADHESION PROTEINS HELP CELLS OF A CERTAIN TYPE STICK TOGETHER TO FORM TISSUES Protein Receptors coming out of plasma membrane What gets in and out of the cell membrane? How do some substances pass through the membrane while others stay out? Materials moves across the membrane in two ways 1. Passive Transport 2. Active Transport PASSIVE TRANSPORT DIFFUSION: MOVEMENT OF MOLECULES FROM HIGH TO LOW CONCENTRATION OXYGEN, CO2, FAT SOLUBLE MOLECULES, AND WATER PASS THROUGH THE MEMBRANE BY SIMPLE DIFFUSION PASSIVE TRANSPORT CARRIER FACILITATED DIFFUSION: DIFFUSION OF LARGE MOLECULES VIA TRANSPORT PROTEINS AQUAPORES: ALLOW WATER TO DIFFUSE IONIC PORES: ALLOW IONS LIKE Na+ or Cl- to diffuse CHANNEL PROTEINS: ALLOW SPECIFIC LARGE MOLECULES TO DIFFUSE LIKE GLUCOSE ACTIVE TRANSPORT ACTIVE TRANSPORT: USING ENERGY TO TRANSPORT LARGE MOLECULES INTO THE CELL AGAINST THEIR CONCENTRATION GRADIENTS 1. CELL MEMBRANE PUMPS CARRIER PROTEINS PUMP IONS AGAINST THEIR CONCENTRATION GRADIENT 2. ENDOCYTOSIS: ENGULFING OF LARGE PARTICLES OR LIQUIDS BY PLASMA MEMBRANE PINOCYTOSIS: ENDOCYTOSIS OF LIQUID PHAGOCYTOSIS: ENDOCYTOSIS OF SOLID CELLS TAKE IN CHOLESTEROL BY ENDOCYTOSIS FROM BLOODSTREAM 3. EXOCYTOSIS:VESICLE CONTENTS EXPELLED BY CELL PROTEINS ARE TRANSPORTED BY EXOCYTOSIS Exocytosis Animation Endocytosis Animation OSMOSIS OSMOSIS: DIFFUSION OF WATER ISOTONIC SOLUTION: CELLS ARE AT EQUILIBRIUM; NO NET MOVEMENT OF WATER HYPERTONIC SOLUTION: CELL IS IN A SOLUTION THAT HAS LOTS OF SALTS OR OTHER IONS IN IT; WATER RUSHES OUT OF THE CELL AND THE CELL SHRINKS HYPOTONIC SOLUTION: CELL IS IN A SOLUTION THAT HAS LITTLE OR NO SALTS OR OTHER IONS IN IT; WATER RUSHES INTO THE CELL AND THE CELL SWELLS Tonicity in Red Blood Cells MIDTERM REVIEW CHAPTER 9 CELLULAR RESPIRATION & PHOTOSYNTHESIS PHOTOSYNTHESIS & CELLULAR RESPIRATION THE BASIC EQUATIONS PHOTOSYNTHESIS + SUNLIGHT CO2 + H20 ======> C6H12O6 + O2 OCCURS IN THE CHLOROPLAST OF AUTOTROPHS CELLULAR RESPIRATION C6H12O6 + O2 ======> CO2 + H20 + ATP OCCURS IN THE MITOCHONDRIA OF ALL CELLS* PHOTOSYNTHESIS + SUNLIGHT CO2 + H20 ======> C6H12O6 + O2 OCCURS IN THE CHLOROPLAST OF AUTOTROPHS HAS BOTH LIGHT AND DARK REACTIONS The LIGHT Reactions + SUNLIGHT CO2 + H20 ======> C6H12O6 + O2 OCCURS IN THE THYLAKOIDS OF CHLOROPLAST LIGHT REACTIONS- Use light to split H20, make O2, and a Hydrogen Ion gradient which makes ATP and NADPH Also known as the LIGHT DEPENDENT REACTIONS The DARK Reactions + ATP + NADPH C H O + O CO2 + H20 ======> 6 12 6 2 OCCURS IN THE STROMA OF CHLOROPLAST The Dark Reactions/Calvin Cycle: Use CO2 , (+ ATP + NADPH) to make C6H12O6 !!! Also known as the LIGHT INDEPENDENT REACTIONS AND OR CALVIN CYCLE!! CELLULAR RESPIRATION OVERVIEW C6H12O6 + O2 ======> CO2 + H20 + ATP OCCURS IN THE MITOCHONDRIA OF ALL CELLS CELLULAR RESPIRATION Has a few Reactions Glycolysis The Krebs/TCA/Citric Acid Cycle The Electron Transport Chain OR Fermentation (if there’s no O2 available) And 2 Major Pathways Aerobic Reactions – use O2 Anaerobic Reactions – Doesn't use O2 CELLULAR RESPIRATION: Glycolysis GLYCOLYSIS C6H12O6 + O2 2ATP + H2O + 2Pyruvate OCCURS IN THE CYTOPLASM!!! NOT MITOCHONDRIA ANAEROBIC REACTION -Doesn’t Use O2 In Short: Step 1 Glycolysis (glucose-lysis) C6H12O62Pyruvate + H2O +2ATP CELLULAR RESPIRATION: THE KREBS CYCLE (*aka. TCA/Citric Acid Cycle) 2 Pyruvate + O2 2ATP + CO2 +2NADH + 2FADH2 OCCURS IN THE MATRIX of MITOCHONDRIA AEROBIC REACTION –USES O2 In Short: Step 2 The Krebs/TCA Cycle 2 Pyruvate CO2 + 2ATP + 2NADH + 2FADH2 CELLULAR RESPIRATION: The ELECTRON TRANSPORT CHAIN The Electron Transport Chain 2NADH+ 2FADH2 H20 + 32-34 ATP OCCURS ON THE CRISTAE OF THE MITOCHONDRIA Uses a Hydrogen Ion gradient to make ATP! In Short: Step 3 The Electron Transport Chain 2NADH + 2FADH2 H20 + 32-34ATP CELLULAR RESPIRATION: If there’s no O2 Organisms use Glycolysis then Fermentation!! ANAEROBIC RESPIRATION- AKA- FERMENTATION 2 TYPES In Animals 2 Pyruvate + 2ATP + Lactic Acid In Yeast 2 Pyruvate + 2ATP + Ethanol In Short: Under Anaerobic Conditons Step 1: Glycolysis Step 2: Fermentation MIDTERM REVIEW CHAPTER 10-11 CELL DIVISION WHY DO CELLS DIVIDE? THEY HAVE GROWN TOO LARGE SO…. THE SURFACE AREA/VOLUME RATIO IS TOO SMALL TO ALLOW AN ORGANISM TO GROW AND INCREASE ITS SIZE CELLS HAVE DIED AND NEED TO BE REPLACED DNA IN THE CELL INSIDE THE NUCLEUS DNA CONDENSES TO FORM CHROMOSOMES CHROMOSOMES ARE MADE FROM PROTEINS CALLED HISTONES AND DNA TOGETHER KNOWN AS CHROMATIN CHROMATIN ALLOWS DNA TO COIL INTO NUCLEOSOMES EACH CHROMOSOME CONSISTS OF IDENTICAL HALVES CALLED SISTER CHROMATIDS SISTER CHROMATIDS ARE JOINED TOGETHER AT THE CENTROMERE NUMBER & TYPES OF CHROMOSOMES SEX CHROMOSOMES DETERMINE THE GENDER OF AN ORGANISM XX = FEMALE XY = MALE DIPLOID CELLS: CELLS WITH 2 COPIES OF EACH CHROMOSOME THE NORMAL BODY CELLS ARE CALLED SOMATIC CELLS, AND THEY ARE ALL DIPLOID DIPLOID & HAPLOID CELLS DIPLOID CELLLS: 2n SOMATIC CELLS HAPLOID CELLS: 1n GAMETES CELL DIVISION IN PROKARYOTES PROKARYOTIC ORGANISMS: a) UNICELLULAR BACTERIA WITH NO NUCLEUS OR MEMBRANE BOUND ORGANELLES b) THEIR DNA IS FOUND AS ONE CIRCULAR CHROMOSOME c) THEIR CELLS REPRODUCE THROUGH BINARY FISSION DNA IS REPLICATED, CELL DOUBLES IN SIZE AND SPLITS STAGES OF MITOSIS 1. PROPHASE a) b) c) 2. METAPHASE a) 3. b) CENTROMERES ARE PULLED APART BY SPINDLE FIBERS AND SISTER CHROMATIDS SPLIT CHROMATIDS MOVE TOWARD OPPOSITE POLES TELOPHASE a) b) c) 5. SPINDLES ALIGN CHROMOSOMES IN THE MIDDLE OF THE CELL AKA METAPHASE PLATE ANAPHASE a) 4. CHROMATIN CONDENSES & NUCLEUS DISAPPEARS SPINDLE FIBERS (MICROTUBULES) FORM & MOVE CHROMOSOMES CENTROSOMES BEGIN TO MIGRATE CHROMOSOMES ARRIVE AT OPPOSITE ENDS OF CELL NUCLEUS REAPPEARS SPINDLE DISAPPEARS CYTOKINESIS a) CELL SPLITS TO FORM 2 NEW DAUGHTER CELLS STAGES OF MITOSIS CYTOKINESIS - PLANT VS. ANIMAL CELLS IN ANIMAL CELLS: A CLEAVAGE FURROW PINCHES ONE CELL INTO 2 CELLS MEIOSIS GETTING FROM DIPLOID TO HAPLOID CELLS: MEIOSIS WHERE DOES IT OCCUR IN HUMANS? MALES: TESTES FEMALES: OVARIES MEIOSIS OVERVIEW MEIOSIS I 1 DIPLOID * CELL SPLITS INTO 2 HAPLOID CELLS MEIOSIS II 2HAPLOID DAUGHTER CELLS UNDERGO MITOSIS FFORMS 4 HAPLOID SEX CELLS SSAME AS MITOSIS MEIOSIS I PROPHASE I HOMOLOGOUS CHROMOSOMES PAIR TOGETHER A PROCESS CALLED SYNAPSIS EACH HOMOLOGOUS PAIR OF CHROMOSOMES IS CALLED A TETRAD PORTIONS OF CHROMATIDS BREAK OFF AND ATTACH TO ADJACENT HOMOLOGOUS CHROMATIDS THIS PROCESS IS KNOWN AS CROSSING OVER CROSSING OVER CREATES NEW GENE COMBINATIONS – THE CHROMOSOMES OF YOUR SEX CELLS ARE COMBINATIONS OF BOTH YOUR MOM AND DADS!!! METAPHASE I HOMOLOGOUS CHROMOSOMES LINE UP IN THE MIDDLE OF THE CELL ANAPHASE I HOMOLOGOUS CHROMOSOMES MOVE TO OPPOSITE POLES OF THE CELL RANDOM SEPARATION OF HOMOLOGOUS CHROMOSOMES IS CALLED INDEPENDENT ASSORTMENT TELOPHASE I CHROMOSOMES REACH OPPOSITE POLES OF THE CELL CYTOKINESIS BEGINS MEIOSIS II 2 HAPLOID CELLS GO THROUGH THE PROCESS OF MITOSIS & CELL DIVISION THE RESULT OF MEIOSIS II IS 4 HAPLOID CELLS GAMETE FORMATION IN MALES MEIOSIS CREATES 4 SPERM CELLS (CALLED SPERMATIDS) IN FEMALES THE CYTOPLASM IS UNEVENLY DIVIDED SO THAT ONLY 1 BIG CELL THE OVUM IS FORMED, ALONG WITH 3 OTHER CELLS KNOWN AS POLAR BODIES MEIOSIS VOCABULARY DIPLOID CELLS: CELLS WITH 2 OF EACH TYPE OF CHROMOSOME (1 FROM MOM AND 1 FROM DAD) N= NUMBER OF PAIRS OF CHROMOSOMES DIPLOID CELLS: 2N ZYGOTE: FERTILIZED EGG GAMETES: HAPLOID SEX CELLS FERTILIZATION: UNION OF EGG AND SPERM SEXUAL REPRODUCTION: PARENTS GENERATE SPECIALIZED SEX CELLS