Introduction • Microbes, or microorganisms, are minute living things that are too small to be seen individually without a microscope • The group includes bacteria, fungi (moulds and yeasts), protozoa and microscopic algae • Viruses are also microorganisms (non cellular – alive or not?) Majority are beneficial • The vast majority of microorganisms are beneficial eg – Marine and freshwater microorganisms – Soil microbes – Photosynthesis – Gut bacteria – Commercial applications – medicines, plastics, food industry Minority are pathogenic • Pathogenic = disease producing • Many diseases caused by microorganisms • Some only affect some groups (elderly, sick, immune compromised) • Most are treatable • Some diseases and epidemics and pandemics have changed the world Naming and classifying • • • • Binomial classification Genus name and specific epithet Both names underlined or italicised By custom, after a scientific name has been mentioned once it can be abbreviated with the initial of the genus followed by the specific epithet • eg Staphylococcus aureus = S. aureus Types of microorganisms • • • • • • Bacteria archaea fungi protozoa algae viruses Bacteria • Singular = bacterium • Relatively simple single celled (unicellular) organisms • Genetic material not enclosed in a special nuclear membrane = prokaryotes • Shapes include rods (bacillus), coccus (spheres) or spiral or even stars or squares Bacteria • May form pairs, chains, clusters or other shapes • Enclosed in peptidoglycan (protein + carbohydrate) cell walls • Generally reproduce by dividing into two equal cells = binary fission • Most use organic chemicals as food • Some can manufacture their own food by photosynthesis • Some ‘eat’ inorganic substances • Many can ‘swim’ using flagella Archaea • Archaea consist of prokaryotic cells but cells walls (if they have them) are not peptidoglycan • Archaea are often found in extreme environments • Archaea are divided into three main groups – Methanogens – Extreme halophiles – Extreme thermophiles Fungi • Fungi are eukaryotes = have a distinct nucleus containing the cell’s genetic material surrounded by a special envelope called the nuclear membrane • Fungi may be unicellular or multicellular • Do not photosynthesise • Cell walls comprised of chitin • Yeasts are unicellular forms of fungi Fungi • Molds are funig that form visible masses called mycelia composed of long filaments (hyphae) • Funig can reproduce sexually or asexually • They get nutrients by absorbing solutions of organic material from their environment • Organisms called slime molds have characteristics of both fungi and amoebas Protozoa • Unicellular eukaryotic microbes • Protozoa move by pseudo pods, flagella or cilia • Protozoa have a variety of shapes and live either as free entities or parasites • Protozoa can reproduce sexually or asexually Algae • Algae are photosynthetic eukaryotes with a wide variety of shapes and both sexual and asexual reproductive forms • Many algae have cell walls made of cellulose • Found in fresh and salt water, in soli and in association with plants • As they photosynthesise they need light, water and carbon dioxide • They produce oxygen and carbohydrates Viruses • • • • Viruses are different to the other microorganisms Very small (electron microscope needed) Acellular (not cellular) Virus particle has a core made up of one type of nucleic acid (RNA or DNA) • The core is covered with a protein coat • Sometimes the coat is covered with an extra lipid layer (called envelope) Why is this strange? • All living cells have RNA and DNA and can carry out chemical reactions and can reproduce as self sufficient units • Viruses can only reproduce by using the cellular machinery of other Whyorganisms is this strange? • Thus viruses are considered to be living when they multiply within the host cells they infect • In this sense viruses are parasites • Viruses are not considered to be living because outside cells they are inert Classification • Before the existence of microorganisms was known, all organisms where grouped into either the animal kingdom or the plant kingdom • When microorganisms were discovered a new system was required • In 1978 Carl Woese devised a system of classification based on the cellular organisation of organisms Three domains 1. Bacteria (cell walls peptidoglycan) 2. Archaea (cell walls, if present, lack peptidoglycan) 3. Eukarya- includes: – Protists (slime molds, protozoa and algae) – Fungi (unicellular yeasts, multicellular molds, mushrooms) – Plants (includes mosses, ferns, conifers, and flowering plants) – Animals (includes sponges, worms, insects and vertebrates)